eIF-2(αP)
A kinase able to phosphorylate exogenous protein synthesis initiation factor eIF-2 alpha is present in lysates of mengovirus-infected L cells.
... 3 0022-538X/86/121012-06$02.00/0 Copyright C 1986, American Society for Microbiology A Kinase Able To Phosphorylate Exogenous Protein Synthesis Initiation Factor eIF-2x Is Present in Lys[r] ...
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Protein Kinase PKR Mediates the Apoptosis Induction and Growth Restriction Phenotypes of C Protein-Deficient Measles Virus
... (19), eIF-2 ␣ is phosphorylated (20, 35), and translation is inhibited (35) in response to MV, but a definitive role for PKR in MV infection has yet to be ...
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AlaArg Motif in the Carboxyl Terminus of the γ134.5 Protein of Herpes Simplex Virus Type 1 Is Required for the Formation of a High-Molecular-Weight Complex That Dephosphorylates eIF-2α
... tein and PP1 was verified by Western blot with mouse mono- clonal anti-His tag antibody, since these proteins were His tagged at the amino terminus (see Fig. 5; also data not shown). To assay for eIF-2␣ ...
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Adenovirus virus-associated RNA and translation control.
... Translational control by adenovirus: lack of virus-associated RNA, during adenovirus infection results in phosphorylation of initiation factor eIF-2 and inhibition of protein synthesis..[r] ...
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Influenza virus regulates protein synthesis during infection by repressing autophosphorylation and activity of the cellular 68,000-Mr protein kinase.
... P68 was immunopurified from infected and, as a control, uninfected cell extracts, and kinase activity was measured with purified eIF-2 as an exogenous Downloaded from http://jvi.asm.org/[r] ...
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The VP35 Protein of Ebola Virus Inhibits the Antiviral Effect Mediated by Double-Stranded RNA-Dependent Protein Kinase PKR
... with eIF-2 ␣ ...on eIF-2 ␣ ...phospho-Ser51 eIF-2 ␣ (Biosource, Inc.) or eIF-2 ␣ (Cell Signaling Technology, ...of eIF-2 ␣ and phosphorylated ...
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Overproduction of Double-Stranded RNA in Vesicular Stomatitis Virus-Infected Cells Activates a Constitutive Cell-Type-Specific Antiviral Response
... in eIF-2 ␣ phosphorylation compared to the wt in L-929 cells at a time when most viral protein synthesis has already taken ...ther 2-aminopurine (2-AP), a PKR inhibitor, nor actinomycin D (act ...
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Internal translation initiation on poliovirus RNA: further characterization of La function in poliovirus translation in vitro.
... To further characterize La activity in translation and to identify other potential limiting factors, we assayed the ability of La protein as well as purified initiation factors, eIF-2, g[r] ...
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Inhibition of Protein Kinase R Activation and Upregulation of GADD34 Expression Play a Synergistic Role in Facilitating Coronavirus Replication by Maintaining De Novo Protein Synthesis in Virus-Infected Cells
... total eIF-2 ␣ was appreciably reduced in IBV-infected cells at late stages of the infection ...total eIF-2 ␣ would render a preferential inhibitory effect on the overall synthesis of host ...
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Herpes Simplex Virus 1 Infection Activates the Endoplasmic Reticulum Resident Kinase PERK and Mediates eIF-2α Dephosphorylation by the γ134.5 Protein
... of ER stress signaling (18, 27, 29, 30, 35). Available evidence indicates that PERK normally exists as an inactive monomer held by ER chaperone proteins GRP78 and GRP94. Under stress conditions, PERK monomers oligomerize ...
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Protein Kinase PKR Plays a Stimulus- and Virus-Dependent Role in Apoptotic Death and Virus Multiplication in Human Cells
... of eIF-2 ␣ at serine 51, which leads to an inhibition of translation ...While eIF-2 ␣ is the best-charac- terized substrate of PKR, additional eIF-2 kinases also are known (HRI, ...
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Antiapoptotic and Oncogenic Potentials of Hepatitis C Virus Are Linked to Interferon Resistance by Viral Repression of the PKR Protein Kinase
... Hepatitis C virus (HCV) is prevalent worldwide and has become a major cause of liver dysfunction and hepatocellular carcinoma. The high prevalence of HCV reflects the persistent nature of infection and the large ...
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The E3L and K3L vaccinia virus gene products stimulate translation through inhibition of the double-stranded RNA-dependent protein kinase by different mechanisms.
... This system exploits the ~g unique property of some plasmid-derived mRNAs to elicit localized DAI kinase activation during COS-1 cell transient transfection, resulting in increased eIF-2[r] ...
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Cell-Type-Specific Growth Restriction of Vesicular Stomatitis Virus polR Mutants Is Linked to Defective Viral Polymerase Function
... and eIF-2 ␣ phosphorylation ...factor eIF-2B required for maintaining eIF-2 ␣ ...that eIF-2 ␣ phosphorylation is enhanced about threefold in polR- infected L-929 cells ...
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Turbulent Flow Measurements near the Discharge Port of a Screw Compressor
... and 2 before and after the opening of the port, ...zone 2 was greatly influenced by the discharge process. In zone 2, both components of mean velocities were subjected to a sudden increase in ...
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Mathematical Model of Cell Growth for Biofuel Production under Synthetic Feedback
... Concentration of pumps pt versus time t for various values of repressor saturation threshold γR, pump activation constant kp, pump degradation rate βp and basal pump production rate αp a[r] ...
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Optimal discrimination between transient and permanent faults
... Suppose now that, for the same processor as in the pre- vious example, the designer decides to run, instead of error detection concurrent with task execution, a reasonably thorough off-line self-testing program, for one ...
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Comparative Study of Millimeter-Wave and THz Performance of DDR IMPATTs Based on Group IV-IV and Group III-V Materials
... Avalanche response times(ƬA) of DDR IMPATTs based on different semiconductors are calculated by Eq.(7).Spatial variations of ionization rates of electrons(αn) and holes(αp) for each dev[r] ...
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A practical implementation of Robust Evolving Cloud based Controller with normalized data space for heat exchanger plant
... where αP , αI , αD , αR are the adaptation gains of the controller parameters, Gsign = ±1 is the known process gain sign, λik is the normalized local density of the cloud, εk is the trac[r] ...
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Efficient simulation of delay in tandem networks using splitting
... the EIF Type 1 and Type 2 methods represent more e cient indicator functions than the PSIF in 11], particularly as the number of tandem queues M is ...The EIF Type 1 method starts to lose e ciency at ...
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