electrophoretic mobility shift
Validation of miRNA mRNA interactions by electrophoretic mobility shift assays
... EMSA or electrophoretic mobility shift assays are widely used to confirm the binding of a nucleic acid to a protein or another nucleic acid [18-20]. However, there have been no reports regarding the ...
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Characterization of nuclear protein binding to a site in the long terminal repeat of a murine leukemia virus: comparison with the NFAT complex.
... competitive electrophoretic mobility shift assays, we demonstrated that the T-cell-specific protein that binds MLPal is identical or similar to a protein from nonactivable T cells that interacts with ...
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Selection of RNA aptamers that bind HIV 1 LTR DNA; strand invaders
... After a total of 10 rounds of selection, the binding of the aptamers to the target dsDNA fragments was assessed using electrophoretic mobility shift assays (Figure 2). The starting pool of RNA from ...
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The Basis for Evolution of DNA-Binding Specificity of the Aft1 Transcription Factor in Yeasts
... performed electrophoretic mobility shift assays with extracts from the aft1Δaft2Δ cells expressing LexA-LtAft and the probe containing the TGCACCC Aft1-binding site of the FET3 ...a mobility ...
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Anti Inflammatory properties of Salograviolide A purified from Lebanese plant Centaurea ainetensis
... EMSA: Electrophoretic Mobility Shift Assay; EDTA: Ethylenediamine Tetraacetic Acid; FBS: Fetal Bovine Serum: HRP: Horse Radish Peroxidase; IBD: Inflammatory Bowel Disease; IKK: Inhibiting IκB Kinase; ...
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In vivo suppression of NF kappa B and preservation of I kappa B alpha by interleukin 10 and interleukin 13
... tion. Electrophoretic mobility shift analysis of nuclear ex- tracts from alveolar macrophages and whole lung tissues demonstrated that both IL-10 and IL-13 suppressed nuclear localization of NF- k B ...
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Sequences at the 3′ Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase
... 1999). Electrophoretic mobility shift and competition assays have now been used to demonstrate that the Escherichia coli-expressed RNA-dependent RNA polymerase domain ( ⌬ 893) derived from BaMV open ...
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Inhibition of calcitriol receptor binding to vitamin D response elements by uremic toxins
... the electrophoretic mobility shift assay to compare the ability of VDRs from normal and renal failure rats to bind to the osteocalcin gene ...
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LncRNA-Safe contributes to cardiac fibrosis through Safe-Sfrp2-HuR complex in mouse myocardial infarction
... RNA electrophoretic mobility shift assay and RNA immunoprecipitation assay indicated that RNA binding protein HuR could bind to Safe-Sfrp2 RNA duplex, whereas the knockdown of HuR dramatically ...
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LncRNA SLCO4A1-AS1 facilitates growth and metastasis of colorectal cancer through β-catenin-dependent Wnt pathway
... Furthermore, we performed RNA electrophoretic mobility shift assay (RNA-EMSA) with biotin-labeled.. 2 SLCO4A1-AS1 knockdown inhibits cell proliferation, migration and invasion in CRC. a [r] ...
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Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Induction by Hypoxia and Hypoxia-Inducible Factors
... FIG 5 HIFs bind to the LANA promoter. (A) HIFs bind to the HRE4R in vitro as detected by electrophoretic mobility shift assay. DIG-labeled synthetic oli- gonucleotide containing wild-type (WT) HRE4R ...
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Hypoxia inducible factor-1 mediates the expression of the immune checkpoint HLA-G in glioma cells through hypoxia response element located in exon 2
... cells. Electrophoretic Mobility Shift Assays and luciferase reporter gene assays revealed that HLA-G upregulation depends on Hypoxia Inducible Factor-1 (HIF-1) and a hypoxia responsive element (HRE) ...
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HBx induced MiR 1269b in NF κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells
... Abbreviations CDC40: cell division cycle 40 homolog; EGFP: enhanced green fluorescent protein; EMSA: electrophoretic mobility shift assay; EMT: epithelial mesenchy‑ mal transitions; HBV:[r] ...
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Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity
... Electrophoretic mobility shift assays EMSA, using the digoxigenin-labeled oligoribonucleotide 3′ UTR-II.1 motif in the presence of the recombinant proteins, were used to analyze the poss[r] ...
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Hypothermia enhances induction of protective protein metallothionein under ischemia
... (A) Electrophoretic mobility shift assay (EMSA) analysis of metal regulatory transcription factor 1 (MTF-1) or metal response elements d (MREd) binding activity was performed using nucle[r] ...
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Müller, Marisa (2009): Characterization of She2p-dependent mRNP assembly in Saccharomyces cerevisiae. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... To test if She2p interacts with these short motifs alone, electrophoretic mobility shift assays (EMSA) were performed. Wild-type She2p was incubated with the respective radiolabeled RN[r] ...
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Molecular mechanism of transcriptional activation of angiotensinogen gene by proximal promoter
... activity. Electrophoretic mobility shift assay and DNase I footprinting demonstrated that the liver- and HepG2-specific nuclear factor (angiotensinogen gene- activating factor [AGF2]) and ubiquitous ...
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Study on Binding Affinity of a Glutathione S Transferase (GST) Fusion Protein to DNA Probe
... by Electrophoretic Mobility Shift ...the electrophoretic transfer; membrane was cross linked and detected by Pierce’s Chemiluminescent Nucleic Acid Detection ...band shift observed in ...
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Integrated Hepatitis B Virus DNA Preserves the Binding Sequence of Transcription Factor Yin and Yang 1 at the Virus-Cell Junction
... In this study, we detected a cellular protein which bound to the IS of HBV DNA at the virus-cell junction in the nuclear extract derived from HepG2 cells and was designated IS bind- ing protein 3 (ISBP3). The amino acid ...
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Expression of Iron Regulatory Protein 1 Is Regulated not only by HIF-1 but also pCREB under Hypoxia
... The inconsistent of responses of IRP1 and HIF-1 alpha to hypoxia and the similar tendencies in the changes of IRP1 and pCREB contents led us to hypothesize that pCREB might be involved in the regulation of IRP1 under ...
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