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Electrophoretic mobility shift assays

Validation of miRNA mRNA interactions by electrophoretic mobility shift assays

Validation of miRNA mRNA interactions by electrophoretic mobility shift assays

... or electrophoretic mobility shift assays are widely used to confirm the binding of a nucleic acid to a protein or another nucleic acid ...

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Electrophoretic mobility shift assays implicate XRCC2:rs3218550C>T as a potential low penetrant susceptibility allele for sporadic breast cancer

Electrophoretic mobility shift assays implicate XRCC2:rs3218550C>T as a potential low penetrant susceptibility allele for sporadic breast cancer

... reporter assays performed in MCF-7 breast cancer cells showed a putative transcriptional repressor effect exerted mainly by the T ...allele. Electrophoretic mobility shift assays were ...

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The Basis for Evolution of DNA-Binding Specificity of the Aft1 Transcription Factor in Yeasts

The Basis for Evolution of DNA-Binding Specificity of the Aft1 Transcription Factor in Yeasts

... performed electrophoretic mobility shift assays with extracts from the aft1Δaft2Δ cells expressing LexA-LtAft and the probe containing the TGCACCC Aft1-binding site of the FET3 ...a ...

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Selection of RNA aptamers that bind HIV 1 LTR DNA; strand invaders

Selection of RNA aptamers that bind HIV 1 LTR DNA; strand invaders

... in electrophoretic mobility shift assays (Lanes a–c correspond to RNA only, RNA + LTR-325 and RNA + LTR-408 bp, ...by electrophoretic mobility shift assays; the ...

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Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Induction by Hypoxia and Hypoxia-Inducible Factors

Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Induction by Hypoxia and Hypoxia-Inducible Factors

... reporter assays in Hep3B cells revealed a moderate activation of the LANA promoter region by hypoxia as well as by cotransfection with degradation-resistant HIF-1 ␣ or HIF-2 ␣ expression ...assay. ...

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Expression of LMP1 in epithelial cells leads to the activation of a select subset of NF-kappa B/Rel family proteins.

Expression of LMP1 in epithelial cells leads to the activation of a select subset of NF-kappa B/Rel family proteins.

... clones, electrophoretic mobility shift assays (EMSA) were performed as previously described by using a labelled oligonucleotide probe containing an NF- k B site that is recognized by most NF- ...

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Characterization of nuclear protein binding to a site in the long terminal repeat of a murine leukemia virus: comparison with the NFAT complex.

Characterization of nuclear protein binding to a site in the long terminal repeat of a murine leukemia virus: comparison with the NFAT complex.

... competitive electrophoretic mobility shift assays, we demonstrated that the T-cell-specific protein that binds MLPal is identical or similar to a protein from nonactivable T cells that ...

7

Hypoxia inducible factor-1 mediates the expression of the immune checkpoint HLA-G in glioma cells through hypoxia response element located in exon 2

Hypoxia inducible factor-1 mediates the expression of the immune checkpoint HLA-G in glioma cells through hypoxia response element located in exon 2

... cells. Electrophoretic Mobility Shift Assays and luciferase reporter gene assays revealed that HLA-G upregulation depends on Hypoxia Inducible Factor-1 (HIF-1) and a hypoxia responsive ...

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Müller, Marisa
  

(2009):


	Characterization of She2p-dependent mRNP assembly in Saccharomyces cerevisiae.


Dissertation, LMU München: Fakultät für Chemie und Pharmazie

Müller, Marisa (2009): Characterization of She2p-dependent mRNP assembly in Saccharomyces cerevisiae. Dissertation, LMU München: Fakultät für Chemie und Pharmazie

... To test if She2p interacts with these short motifs alone, electrophoretic mobility shift assays (EMSA) were performed. Wild-type She2p was incubated with the respective radiolabeled RN[r] ...

132

Molecular mechanism of transcriptional activation of angiotensinogen gene by proximal promoter

Molecular mechanism of transcriptional activation of angiotensinogen gene by proximal promoter

... (CAT) assays with 5'-deleted constructs showed that the proximal promoter region from -96 to +22 of the transcriptional start site was enough to express HepG2-specific CAT ...activity. Electrophoretic ...

11

Expression of Iron Regulatory Protein 1 Is Regulated not only by HIF-1 but also pCREB under Hypoxia

Expression of Iron Regulatory Protein 1 Is Regulated not only by HIF-1 but also pCREB under Hypoxia

... immunofluorescence, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation ...ChIP assays demonstrated that pCREB binds to IRP1 promoter; and 8) HIF-1 and/or HIF-2 siRNA ...

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Sequences at the 3′ Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase

Sequences at the 3′ Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase

... 1999). Electrophoretic mobility shift and competition assays have now been used to demonstrate that the Escherichia coli-expressed RNA-dependent RNA polymerase domain ( ⌬ 893) derived from ...

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A STUDY OF THE NATURE OF THE CIRCULATING THYROID HORMONE IN EUTHYROID AND HYPERTHYROID SUBJECTS BY USE OF PAPER ELECTROPHORESIS

A STUDY OF THE NATURE OF THE CIRCULATING THYROID HORMONE IN EUTHYROID AND HYPERTHYROID SUBJECTS BY USE OF PAPER ELECTROPHORESIS

... The rapid shift of I131 to the protein fraction having electrophoretic mobility slightly faster than alpha-2 globulin sections 9 and 10 in the hyperthyroid patients and the slower but si[r] ...

7

Atf2 Transcription Factor Binds to the APP1 Promoter in Cryptococcus neoformans: Stimulatory Effect of Diacylglycerol

Atf2 Transcription Factor Binds to the APP1 Promoter in Cryptococcus neoformans: Stimulatory Effect of Diacylglycerol

... (A) Electrophoretic gel stained with ethidium bromide showing the DNA fragment of ⬃ 250 bp corresponding to the APP1 promoter (PCR 26 cycles, lane 1) after the immunoprecipitation (After IP) of DNA using anti-HA ...

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Identification and characterization of regulatory elements in the promoter of ACVR1, the gene mutated in Fibrodysplasia Ossificans Progressiva

Identification and characterization of regulatory elements in the promoter of ACVR1, the gene mutated in Fibrodysplasia Ossificans Progressiva

... To complete our analysis on the involvement of the Sp1 transcription factor in ACVR1 basal promoter regulation, we carried out an Electrophoretic Mobility Shift Assay (EMSA) by using a 3[r] ...

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Anti Inflammatory properties of Salograviolide A purified from Lebanese plant Centaurea ainetensis

Anti Inflammatory properties of Salograviolide A purified from Lebanese plant Centaurea ainetensis

... The biological activity of SA could be due to the presence of α,β-unsaturated carbonyl structures mainly the α- methylene-γ-lactone. These structures have the ability to react with nucleophiles by a Michael-type addition ...

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Integrated Hepatitis B Virus DNA Preserves the Binding Sequence of Transcription Factor Yin and Yang 1 at the Virus-Cell Junction

Integrated Hepatitis B Virus DNA Preserves the Binding Sequence of Transcription Factor Yin and Yang 1 at the Virus-Cell Junction

... In this study, we focused on the 54-kDa protein binding to integrated HBV DNA at the virus-cell junction. This protein has been detected as ISBP3 in the nuclear extract prepared from HepG2 cells using E64-containing ...

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CTGC motifs within the HIV core promoter specify Tat responsive pre initiation complexes

CTGC motifs within the HIV core promoter specify Tat responsive pre initiation complexes

... function studies of USF-1 [43,44], we introduced a CACGTG E-box (Figure 6A, “USF-1+”), with increased affinity for USF-1 with respect to the wild type HIV E- box, CAGCTG. BLAST searches showed that the CACGTG E-box is ...

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LncRNA-Safe contributes to cardiac fibrosis through Safe-Sfrp2-HuR complex in mouse myocardial infarction

LncRNA-Safe contributes to cardiac fibrosis through Safe-Sfrp2-HuR complex in mouse myocardial infarction

... RNA electrophoretic mobility shift assay and RNA immunoprecipitation assay indicated that RNA binding protein HuR could bind to Safe-Sfrp2 RNA duplex, whereas the knockdown of HuR dramatically ...

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Two adjacent nuclear factor binding domains activate expression from the human PRNP promoter

Two adjacent nuclear factor binding domains activate expression from the human PRNP promoter

... with mobility shift electrophoresis and methylation interference assays indicated the presence of sequence specific nuclear factor complexes that bind to the prpA and prpB domains and activate ...

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