Entry inhibitors
Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
... assay entry mediated by HIV-1 Env and can be used to evaluate HIV-1 entry inhibitors, such as small molecules or neutralizing antibodies in sera of vaccinated animals or ...
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New Small Molecule Entry Inhibitors Targeting Hemagglutinin-Mediated Influenza A Virus Fusion
... for inhibitors of the HA-mediated entry process by using influenza pseudotype virus [HIV/HA(H5)] according to previ- ously described methods (31, 35, ...virus entry was quantified by measuring the ...
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Kinetic Factors Control Efficiencies of Cell Entry, Efficacies of Entry Inhibitors, and Mechanisms of Adaptation of Human Immunodeficiency Virus
... of entry inhibitors on efficiencies and kinetics of ...the inhibitors used for the studies described above, we employed our cell clones that express low or high concentrations of wild-type ...
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Topical application of entry inhibitors as "virustats" to prevent sexual transmission of HIV infection
... for entry inhibitors of different classes, leading many investi- gators to speculate that these observations reflect some intrinsic difficulty in delivering inhibitors to sites where they need to act ...
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Virus Isolates during Acute and Chronic Human Immunodeficiency Virus Type 1 Infection Show Distinct Patterns of Sensitivity to Entry Inhibitors
... of entry inhibitors in suppressing virus strains at dif- ferent disease stages but also aimed to gain information on whether in vivo selection pressures exist that affect the inter- action of the virus with ...
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Viral Entry Inhibitors Targeted to the Membrane Site of Action
... HIV entry inhibitors ...viral entry is inhib- ited more effectively by cholesterol-tagged peptides than by untagged ...Viral entry is assessed here by a plaque reduction assay, in which each ...
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Screening and Identification of Lassa Virus Entry Inhibitors from an FDA-Approved Drug Library
... LASV entry by using pseudotype virus bearing LASV envelope glycoprotein ...LASV entry inhibitors in the micromolar ...LASV entry by blocking low-pH-induced membrane ...an entry-targeted ...
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Identification and Characterization of Influenza Virus Entry Inhibitors through Dual Myxovirus High-Throughput Screening
... different entry inhibitors identified the neighboring residue 59 as a resistance hot spot (45, ...all entry inhibitor classes that were profiled in regard to resistance ...
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Lysosomotropic agents as HCV entry inhibitors
... Hepatitis C Virus (HCV) is an enveloped, positive stranded RNA virus classified in the family of Flaviviri- dae. HCV causes acute and chronic hepatitis infection which can eventually lead to permanent liver damage, ...
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Molecular Determinants of Antiviral Potency of Paramyxovirus Entry Inhibitors
... peptide inhibitors (NiV FC2 ...pseudotyped-virus entry in a new assay that mimics multicycle ...infection, entry into the target cells in the absence and presence of the original and mu- tated HRC ...
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Identification of Ebola Virus Inhibitors Targeting GP2 Using Principles of Molecular Mimicry
... Visualization of MD trajectories was conducted using VMD (99) and Chimera (88). The AMBER14 accessory program cpptraj (80) and in-house protocols were utilized to extract VDW and ES energies (with distance-dependent ...
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Characterization of resistance to a potent d peptide HIV entry inhibitor
... Q577R has also been identified in viral resistance screens against other HIV-1 entry inhibitors that target the prehairpin intermediate. Against N-trimer peptides N44, N36, and IZN36, the C-peptide T2635, ...
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Baseline Resistance of Primary Human Immunodeficiency Virus Type 1 Strains to the CXCR4 Inhibitor AMD3100
... The entry of human immunodeficiency virus type 1 (HIV-1) into cells can be prevented by a variety of small-molecule inhibitors that target the viral envelope (Env) protein or the coreceptors to which it ...
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Identification of Piperazinylbenzenesulfonamides as New Inhibitors of Claudin-1 Trafficking and Hepatitis C Virus Entry
... Activation of the PKA pathway by either forskolin treatment, overexpression of the exogenous GPCR 5-HT6, or overexpression of the PKA catalytic subunit itself increased CLDN1 export at the surface or rescued its surface ...
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Non-Hepatic Adverse Effects of Antiretroviral Therapy for HIV Treatment and Care
... Some of the C-C chemokine receptors on CD4+ T cells include CCR5 (R5) and CXCR4 (X4). Maraviroc, which is an entry inhibitor is a CCR5 antagonist and is used in both treatment-naïve and treatment- experienced HIV ...
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Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site
... The inhibitor was situated in the protein’ s nuclease active site with the N-hydroxyurea moiety directly coordinating two Mg 2+ ions positioned 4.5 Å apart (Figure 1b), anchored by inner-sphere metal-coordinating ...
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Cellular Active N-Hydroxyurea FEN1 Inhibitors Block Substrate Entry to the Active Site
... 82 83 Results 84 N-Hydroxyurea hFEN1 inhibitors bind catalytic site metals 85 Inhibitor 124 Figure 1a was co-crystallized with hFEN1–Mg2+ truncated after residue 336 86 hFEN1-336Δ, which[r] ...
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Will Multiple Coreceptors Need To Be Targeted by Inhibitors of Human Immunodeficiency Virus Type 1 Entry?
... There is strong genetic evidence that CCR5 is the most important coreceptor for the macrophage-tropic viruses that are commonly transmitted between individuals (13, 27, 31, 44). There is also good circumstantial evidence ...
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Peptide Inhibitors of Flavivirus Entry Derived from the E Protein Stem
... Preconcentration of a fusion inhibitor in the membrane of an enveloped virus is likely to be a general mechanism for enhancing inhibitory activity. Certain broadly neutralizing an- tibodies that bind the ...
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Enhanced human enterovirus 71 infection by endocytosis inhibitors reveals multiple entry pathways by enterovirus causing hand foot and mouth diseases
... receptor-limited cell types [36]. Another neurotropic virus, herpes simplex virus can use different endocy- tic route to infect Hela cells, cultured epiderm and neurons [37, 38]. Consistently, we found here that a novel ...
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