enzyme-linked immunosorbent assays
Specific Enzyme-Linked Immunosorbent Assays for Quantitation of Antibody-Cytokine Fusion Proteins
... Preliminary testing has shown in vitro and in vivo that antitumor activity can be obtained with fusion proteins linking tumor-reactive monoclonal antibodies to cytokines, such as granulocyte-macrophage colony- ...
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Novel enzyme-linked immunosorbent assays for the detection of anti-Fc gamma receptor autoantibodies.
... There is a substantial interest in the role of antineutrophil antibodies since Fc gamma receptors (Fc g Rs) have been identified as the target for the majority of such autoantibodies. Antineutrophil antibodies have long ...
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Serologic Diagnosis of Lyme Borreliosis by Using Enzyme Linked Immunosorbent Assays with Recombinant Antigens
... Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Bor- relia burgdorferi sensu stricto and Western blot analyses with whole cells of this ...
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Evaluation of Recombinant Leptospira Antigen Based Enzyme Linked Immunosorbent Assays for the Serodiagnosis of Leptospirosis
... 47). Enzyme-linked immunosorbent assays (ELISAs) (12, 46, 48), and other rapid serologic tests based on whole-cell leptospiral antigen preparations (43, 44, 50) have been devel- oped for use ...
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Evaluation of rubella immune status by three commercial enzyme linked immunosorbent assays
... Three commercial indirect enzyme-linked immunosorbent assays ELISAs Enzygnost-Rubella, RUBELISA, and ORTHO Rubella were evaluated for the determination of immune status by testing 1,090 [r] ...
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Performance of Commercial Enzyme Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis
... Measuring antibodies to Bordetella pertussis antigens is mostly done by enzyme-linked immunosorbent assays (ELISAs). We compared the performance of ELISA kits that were commercially available ...
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Comparison of Five Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis
... Measuring antibodies to Bordetella pertussis antigens is mostly done by enzyme-linked immunosorbent assays (ELISAs). We compared the performance of five commercially available ELISA kits with ...
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Development of Rapid Enzyme Linked Immunosorbent Assays for Detection of Antibodies to Burkholderia pseudomallei
... rapid enzyme-linked immunosorbent assays (ELISAs) were developed using two purified polysaccharide antigens (O-polysaccharide [OPS] and 6-deoxyheptan capsular polysaccharide [CPS]) and two ...
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Chromatographic purification and characterization of antigens A and D from Mycobacterium paratuberculosis and their use in enzyme linked immunosorbent assays for diagnosis of paratuberculosis in sheep
... The characterization of antigen A is shown here, and both antigens were evaluated along with lipoarabinomannan antigen in indirect enzyme-linked immunosorbent assays ELISA for the serodi[r] ...
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Use of Western Blot and Enzyme-Linked Immunosorbent Assays to Assist in the Diagnosis of Lyme Disease
... In a related study from Connecticut, of 1149 serologically confirmed Lyme disease pa tients 20 years of age or younger, only 10% of those with arthritis also had evidence of ECM.7 Simila[r] ...
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Validity and application of immunoturbidimetric and enzyme-linked immunosorbent assays for the measurement of adiponectin concentration in ponies
... For the IT assay, serum samples of known adiponectin concentration were pooled into high and low pool samples (n = 3 samples/group), respectively. Each pooled sample was analysed three times within a run on each of three ...
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Microsticks as solid phase carriers for enzyme linked immunosorbent assays
... Next, stainless steel, polystyrene, and teflon microsticks were coated with one of the polycarbonate or nitrocellulose materials and sensitized with the test antigens.. The results of as[r] ...
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Enzyme linked immunosorbent assays for Staphylococcus aureus exfoliative toxins A and B and some applications
... Our report describes two simple, specific, and sensitive 3 ng/ml ELISA procedures which require affinity-purified toxin antibodies and which can be used to determine the ET concentration[r] ...
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Serodiagnosis of Helicobacter pylori: comparison of enzyme linked immunosorbent assays
... We therefore prospectively evaluated different ELISAs developed in the United States and the United Kingdom by using as a "gold standard" the results from histology and culture of gastri[r] ...
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Evaluation of Three Commercial Enzyme Linked Immunosorbent Assays for Diagnosis of Chagas’ Disease
... three assays gave satisfactory results with sera which were obtained in four Brazilian areas and classified as either consensus positive or negative by matched in-house IFA and in-house ELISA ...
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Evaluation of two photometers used in enzyme linked immunosorbent assays
... Since both of these photometers use a vertical light path for through-the-plate reading, the linearity of instrumental reading was verified by regression analysis of the absorbance curve[r] ...
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Detection of human calicivirus antigen and antibody by enzyme linked immunosorbent assays
... A total of 90 stool samples including 23 samples used to determine the range of negative results that were collected from hospitalized infants with diarrhea and that lacked any detectabl[r] ...
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Enzyme linked immunosorbent assays adapted for serotyping of human rotavirus strains
... For ELISA 1, 2, and 5, the neutralizing titer was expressed as the highest dilution of the typespecific antiserum IS, and IS2 able to detect a constant amount of antigen in a 7.5% stool [r] ...
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Glycoprotein Based Enzyme Linked Immunosorbent Assays for Serodiagnosis of Infectious Laryngotracheitis
... The detection of the humoral immune response is critical for the rapid identification of ILTV-infected birds (5). The enzyme- linked immunosorbent assay (ELISA) has been used for the detec- tion of ...
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Enzyme linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection
... Three sera were evaluated: i a hyperimmune guinea pig anti-rotavirus serum obtained from the National Institutes of Health, Bethesda, Md., produced under contract for the World Health Or[r] ...
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