epitope mapping
Comparative characteristic of the methods of protein antigens epitope mapping
... of epitope mapping of protein antigens has been car - ried ...of epitope searching algorithms were integrated into a computer program, which greatly facilitates the analysis of experimental data and ...
14
Characterization and Epitope Mapping of the Polyclonal Antibody Repertoire Elicited by Ricin Holotoxin-Based Vaccination
... vaccination. Epitope mapping analysis using phage display random peptide libraries revealed that the polyclonal serum contains four immunodominant epitopes, three of which are located on the A subunit and ...
7
In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate
... Detailed epitope mapping of MAEBL through immunoinformatics identified several MHCI, MHCII and B cell epitopes throughout the peptide, with several of these lying in the M2 domain and being conserved ...
9
T cell subsets, epitope mapping, and HLA restriction in patients with allergic bronchopulmonary aspergillosis
... Allergic bronchopulmonary aspergillosis (ABPA) is a hy- persensitivity lung disease characterized by Aspergillus fu- migatus (Af) colonization, IgE and IgG anti-Af antibodies, pulmonary infiltrates, bronchiectasis, and ...
9
Epitope Mapping of the Brucella melitensis BP26 Immunogenic Protein: Usefulness for Diagnosis of Sheep Brucellosis
... The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the ...
5
B Cell Epitope Mapping of the VapA Protein ofRhodococcus equi: Implications for Early Detection ofR equi Disease in Foals
... the epitope mapping of Mycoplasma bovis variable surface lipoproteins have shown B-cell epitopes to be around three to seven amino acid resi- dues long ...11-mer epitope NLQKDEPNGRA described ...
5
Epitope Mapping of HIV-Specific CD8+ T Cell Responses by Multiple Immunological Readouts Reveals Distinct Specificities Defined by Function
... that epitope specificity differs between short- and long-term assays as well as within the short-term ...defining epitope specificity is one that could be used in vaccine evaluations in order to deter- mine ...
12
Epitope mapping of PfCP 2 9, an asexual blood stage vaccine candidate of Plasmodium falciparum
... lar proteolytic processing step by P. falciparum subtilisin- like protease 2 (PfSUB2) [4,5], their C-terminal regions remain anchored within the parasite membrane, and then are carried into the newly invaded red blood ...
12
Epitope mapping by random peptide phage display reveals essential residues for vaccinia extracellular enveloped virion spread
... approach was used. In this approach, the randomized peptide segment is flanked by paired cysteines, which are oxidized during phage assembly to present the pep- tide as a taut loop at the N-terminus of the minor phage ...
11
Epitope Mapping of Ibalizumab, a Humanized Anti-CD4 Monoclonal Antibody with Anti-HIV-1 Activity in Infected Patients
... ibalizumab epitope by point mutations in chimeric mCD4-hGACFG or in ...ibalizumab epitope, we introduced single amino acid changes in the chimeric mCD4-hGACFG molecule ...ibalizumab epitope is mainly ...
8
Production, characterization, and epitope mapping of a monoclonal antibody against genotype VII Newcastle disease virus V protein
... Expression and purification of recombinant NDV ZJ1-VCD protein A His-tagged form of NDV V protein CTD polypeptide was highly expressed by pET28a-ZJ1/VCD in E.. coli and had a molecular w[r] ...
20
Epitope mapping and characterization of the infectious hematopoietic necrosis virus glycoprotein, using fusion proteins synthesized in Escherichia coli.
... The resulting plasmids, pXL2, pXL3, and pXL7, encoded trpE-G fusion proteins subsequently detected with anti-infectious hematopoietic necrosis virus sera by Western immunoblots.. A compa[r] ...
5
Characterization and Epitope Mapping of Neutralizing Monoclonal Antibodies Produced by Immunization with Oligomeric Simian Immunodeficiency Virus Envelope Protein
... exposed epitope and thus less likely to be affected by oligomerization status, we set the average OD for V3 loop MAbs 36D5 and 83F3 against gp120 and sgp140 at a value of 1 and calculated the reactivity of the ...
14
Epitope-Mapping Studies Define Two Major Neutralization Sites on the Vaccinia Virus Extracellular Enveloped Virus Glycoprotein B5R
... Localization of antigenic sites involved in neutralization gives clues as to functional regions of the molecule. Law and Smith (17) proposed that the neutralization epitopes of B5R were within SCR1. Our data suggest that ...
12
Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure
... Recombinant adeno-associated virus type 2 (AAV) is a common vector used in human gene therapy proto- cols. We characterized the humoral immune response to AAV and observed that 80% of normal human subjects have anti-AAV ...
6
Protocol. PepSpot Peptides. Epitope Mapping with synthetic peptides prepared by SPOT-Synthesis. Contact us: Product Use & Liability
... The techniques described are primarily used for the mapping of monoclonal antibody epitopes. Analogous procedures can be applied for the characterization of polyclonal antibodies or patients sera (Valle 1999). In ...
24
Epitope Mapping of the HSP83.1 Protein of Leishmania braziliensis Discloses Novel Targets for Immunodiagnosis of Tegumentary and Visceral Clinical Forms of Leishmaniasis
... These peptides and the recombinant heat shock protein 83.1 (rHSP83.1) were tested in enzyme-linked immunosorbent assays (ELISAs) against serum samples from patients with tegumentary leis[r] ...
11
Epitope Mapping of the Hemagglutinin Molecule of A/(H1N1)pdm09 Influenza Virus by Using Monoclonal Antibody Escape Mutants
... Characteristics of the antigenic structure of pdm09 HA. Fig- ure 2 shows the antigenic sites of A/Narita/1/2009 HA (Fig. 2). The epitopes for the investigated MAbs, except for epitope n2, were localized within the ...
10
Fine epitope mapping of the human SS B/La protein Identification of a distinct autoepitope homologous to a viral gag polyprotein
... immunosorbent assay were used to evaluate the reactivities of the recombinant proteins with patients' sera to determine the autoepitopes. A narrow segment (amino acid 88-101) and the region where several epitopes were ...
10
Extraction, texture analysis and polysaccharide epitope mapping data of sequential extracts of strawberry, apple, tomato and aubergine fruit parenchyma
... overnight at 4 ° C. The ELISA analysis was performed following the protocol described in [3]. The analysis of each sample was performed in triplicate. Due to varied antibody avidities, direct quanti- tative comparisons ...
7