exonic splicing enhancer (ESE)
Selection of the bovine papillomavirus type 1 nucleotide 3225 3' splice site is regulated through an exonic splicing enhancer and its juxtaposed exonic splicing suppressor.
... Alternative splicing is an important mechanism for the regulation of bovine papillomavirus type 1 (BPV-1) gene expression during the virus life ...vitro splicing in HeLa cell nuclear extracts to identify ...
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Regulation of vif mRNA Splicing by Human Immunodeficiency Virus Type 1 Requires 5′ Splice Site D2 and an Exonic Splicing Enhancer To Counteract Cellular Restriction Factor APOBEC3G
... from splicing of the major splice donor in the HIV-1 genome, 5 ⴕ splice site (5 ⴕ ss) D1, to the first splice acceptor, 3 ⴕ ss ...that splicing of HIV-1 vif mRNA is tightly regulated by suboptimal 5 ⴕ ss ...
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Exonic Splicing Enhancer-Dependent Selection of the Bovine Papillomavirus Type 1 Nucleotide 3225 3′ Splice Site Can Be Rescued in a Cell Lacking Splicing Factor ASF/SF2 through Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway
... and exonic splicing enhancer-dependent splicing at the proximal 3⬘ splice site in the cells lacking ASF/SF2, protein samples prepared from the DT40-ASF-BPV-1-1 cells were blotted for analysis ...
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The mechanisms of a mammalian splicing enhancer
... Exonic splicing enhancer (ESE) sequences are bound by serine & arginine-rich (SR) proteins, which in turn enhance the recruitment of splicing ...of splicing around twenty years ago ...
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A targeted oligonucleotide enhancer of SMN2 Exon 7 splicing forms competing quadruplex and protein complexes in functional conditions
... the splicing of selected exons is limited by a poor understanding of the mechanisms ...oligonucleotide enhancer of splicing (TOES) anneals to SMN2 exon 7 and carries an exonic splicing ...
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Conservation of CD44 exon v3 functional elements in mammals
... alternative splicing route different from that affecting other variable exons so it can be included in the mRNA together with other variable exons or independently from them ...bipartite exonic ...
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An Exonic Splicing Silencer Downstream of the 3′ Splice Site A2 Is Required for Efficient Human Immunodeficiency Virus Type 1 Replication
... unique splicing pattern since it includes an exon within the env gene (exon 6), through utilization of cryptic 3 ⬘ ss A6 ...overall splicing of viral RNA was increased, which was correlated with decreased ...
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Serine/Arginine-Rich Splicing Factor 3 and Heterogeneous Nuclear Ribonucleoprotein A1 Regulate Alternative RNA Splicing and Gene Expression of Human Papillomavirus 18 through Two Functionally Distinguishable cis Elements
... RNA splicing to produce a repertoire of viral transcripts for the expression of individual viral ...RNA splicing re- main unknown. In this study, an exonic splicing enhancer (ESE) in ...
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Structural, functional, and protein binding analyses of bovine papillomavirus type 1 exonic splicing enhancers.
... BPV-1 expression vector and transformation mutagenesis. The BPV-1 late gene expression vector CCB458-2 (p3231) contains two fragments of BPV-1 DNA (nt 7254 to 7563 and nt 3073 to 7356) that were joined by PCR overlap ...
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Optimization of a Weak 3′ Splice Site Counteracts the Function of a Bovine Papillomavirus Type 1 Exonic Splicing Suppressor In Vitro and In Vivo
... Alternative splicing is a critical component of the early to late switch in papillomavirus gene ...viral splicing elements have recently been identified between the two alternative 3 ⴕ splice sites and have ...
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Utilization of the Bovine Papillomavirus Type 1 Late-Stage-Specific Nucleotide 3605 3′ Splice Site Is Modulated by a Novel Exonic Bipartite Regulator but Not by an Intronic Purine-Rich Element
... alternative splicing identified three cis-acting elements between these two splice ...purine-rich exonic splicing enhancers, SE1 and SE2, are essential for preferential utilization of the nt 3225 3 ⴕ ...
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Identification of functional long non-coding RNAs in C. elegans
... with enhancer RNAs rarely being spliced [29]. In contrast, multi-exonic loci were only enriched for active enhancers during early embryonic stage ...
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Grammar systems in simple splicing
... Chapter 4 discusses thoroughly on the simple splicing grammar systems (SSGS). Various types of SSGS are observed. Besides that, this chapter also discussed on the concept of pattern and pure pattern grammar. Then, ...
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A splicing enhancer in the 3'-terminal c-H-ras exon influences mRNA abundance and transforming activity.
... With the exception of the first half of human intron E, the four H-ras sequences are similar throughout, which was previ- ously noted for the human and rat genes by Ruta et al., who also indicated that this level of ...
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The Alternative Splicing Mutation Database: a hub for investigations of alternative splicing using mutational evidence
... Alternative Splicing Mutation Database (ASMD) – a collection of human exon sequences with short (1–6 nucleotides) internal mutations that change the balance of alternatively spliced mRNA isoforms or cause the ...
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Intron Definition Is Required for Excision of the Minute Virus of Mice Small Intron and Definition of the Upstream Exon
... the splicing machinery to choose the poorer donor (D1), which would be more consis- tent with an intron-defined ...cis-acting splicing signals are highly context dependent ...
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Splicing of the Large Intron Present in the Nonstructural Gene of Minute Virus of Mice Is Governed by TIA-1/TIAR Binding Downstream of the Nonconsensus Donor
... probes spanning MVM nt 1858 to 2377, and the results were quantified using a Fuji FLA 5000 phosphorimager as previ- ously described (20, 24). These mutations change the coding sequence for NS1 (and thus the capsid P38 ...
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Exonic DNA Sequencing of ERBB4 in Bipolar Disorder
... This study has several limitations, perhaps the most important being the relatively small sample size. Assuming a binomial distribution, our sample of 188 sequenced cases had an 80% probability to find a mutation with a ...
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Calculation of Splicing Potential from the Alternative Splicing Mutation Database
... in splicing that is based solely on the ASMD ...as splicing enhancers (having positive SP i values) and in other cases as splicing silencers (with negative SP i ...on splicing mutations should ...
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spp42, Identified as a Classical Suppressor of prp4-73, Which Encodes a Kinase Involved in Pre-mRNA Splicing in Fission Yeast, Is a Homologue of the Splicing Factor Prp8p
... gene named prp8/cdc28 encodes a putative helicase, we replated the cells transformed with the three differ- which is possibly involved in pre-mRNA splicing and ent plasmids onto nonselective medium. Again, only ...
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