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Expression of outer membrane proteins

Temperature-regulated expression of outer membrane proteins in Shigella flexneri

Temperature-regulated expression of outer membrane proteins in Shigella flexneri

... the expression of microbial ...how outer membrane proteins (OMPs) of the bacterium Shigella flexneri respond to stress, especially during fever when the host ’ s body temperature is ...

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Sorting and membrane integration of mitochondrial outer membrane proteins

Sorting and membrane integration of mitochondrial outer membrane proteins

... Although pATOM36 and Mim1/2 do not share any sequence or topological similarities (Figure 1— figure supplement 1), we wondered whether convergent evolution allowed these unrelated proteins to fulfil similar tasks ...

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Structural and functional studies of bacterial outer membrane proteins

Structural and functional studies of bacterial outer membrane proteins

... routine: expression, purification, crystallization and X-ray data ...solve membrane protein structures? The barriers impact upon every step of structural ...recombinant expression and the following ...

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Helicobacter pylori outer membrane proteins and gastroduodenal disease

Helicobacter pylori outer membrane proteins and gastroduodenal disease

... Overall, because of the technical difficulties in evaluating OMPs status by PCR, we suggest that currently the most reliable method is to confirm protein expression by immuno- blot. That said, there are important ...

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The Role of Outer Membrane Proteins of Porphyromonas gingivalis in Host-Pathogen Interactions

The Role of Outer Membrane Proteins of Porphyromonas gingivalis in Host-Pathogen Interactions

... the outer membrane proteins, some of which are thought to be involved in invasion, such as the OmpA ...their expression, such as oxyR and sodB, which may be needed in response to the change to ...

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Outer membrane proteins and morphological alterations of Shigella spp. under starvation in seawater

Outer membrane proteins and morphological alterations of Shigella spp. under starvation in seawater

... Outer membrane profiles of Shigella starved eight months in seawater does not show a very large variation from the normal ...their expression was ...level expression of one band corresponding ...

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Defining the Roles of Outer Membrane Proteins and Host Cathelicidins in Uropathogenic Escherichia coli Infection

Defining the Roles of Outer Membrane Proteins and Host Cathelicidins in Uropathogenic Escherichia coli Infection

... controls expression of the thrLABC operon, an operon encoding four of the five threonine biosynthesis enzymes [33, 56, ...the expression of the differentially regulated UTI89 genes within MG1655 cells ...

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In Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties

In Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties

... The outer membrane vesicle (OMV) of Neisseria meningitidis is among the newly studied components with microbial origin, which could be applied as an ...predominant outer membrane ...

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Outer Membrane Proteins and DNA Profiles in Strains of Haemophilus parasuis Recovered from Systemic and Respiratory Sites

Outer Membrane Proteins and DNA Profiles in Strains of Haemophilus parasuis Recovered from Systemic and Respiratory Sites

... Results showed that strains recovered from systemic sites had more homogeneous OMP and DNA profiles than those isolated from respiratory sites. Evaluation of isolates involved in the multifarm outbreak showed that only ...

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Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins

Inflammasome Activation by Bacterial Outer Membrane Vesicles Requires Guanylate Binding Proteins

... Induction of pyroptosis and interleukin secretion by OMVs requires GBPs. Previous studies proposed that GBPs extract LPS from intracellular bacterial pathogens through PV lysis and bacteriolysis, processes that require ...

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Identification of a ubiquitous family of membrane proteins and their expression in mouse brain

Identification of a ubiquitous family of membrane proteins and their expression in mouse brain

... human proteins were identified; the MUSTETU protein is the homologue of mouse TMS-2, and Diff33 is homologous to ...All proteins encoded by the ORF of the TMS family encode proteins of 400–450 amino ...

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AupA and AupB Are Outer and Inner Membrane Proteins Involved in Alkane Uptake in Marinobacter hydrocarbonoclasticus SP17

AupA and AupB Are Outer and Inner Membrane Proteins Involved in Alkane Uptake in Marinobacter hydrocarbonoclasticus SP17

... two proteins, AupA and AupB, which are required for growth on alkanes in the marine hydrocar- bonoclastic bacterium Marinobacter ...whose expression was increased upon adhesion to and biofilm for- mation on ...

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tRNAs and proteins use the same import channel for translocation across the mitochondrial outer membrane of trypanosomes

tRNAs and proteins use the same import channel for translocation across the mitochondrial outer membrane of trypanosomes

... by expression of a fusion protein comprised of the N- terminal 160 aa of mitochondrial LDH fused to mouse dihydrofolate reductase (DHFR) followed by a C-terminal hemagglutinin (HA)-tag we can produce an import ...

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Discrimination of outer membrane proteins with improved performance

Discrimination of outer membrane proteins with improved performance

... 44 proteins were predicted to be TMB proteins by BOMP but not by our ..."Cell membrane" or "multi-pass membrane protein" in ...OMP proteins missed by the ...

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Virulent strain associated outer membrane proteins of Borrelia burgdorferi

Virulent strain associated outer membrane proteins of Borrelia burgdorferi

... purified outer membrane vesicles (OMV) from Borrelia burgdorferi strain B31 based on methods developed for isolation of Treponema pallidum ...their outer membrane origin and their lack of ...

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Microbial expression systems for membrane proteins

Microbial expression systems for membrane proteins

... eukaryotic membrane proteins in microbial ...recombinant proteins being produced in human cells and tuning human promoters to favour efficient folding is still in its ...

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Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

Two outer membrane proteins are bovine lactoferrin-binding proteins in Mannheimia haemolytica A1

... As the results with both strains of M. haemolytica were similar in the binding to BLf, only the MhF strain OMP were used to perform 2-D electrophoresis. The OMP sepa- rated by 2-D electrophoresis were transferred to a ...

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Identification and Structural Characterisation of Novel Outer Membrane Proteins in the genus Borrelia

Identification and Structural Characterisation of Novel Outer Membrane Proteins in the genus Borrelia

... OmpA-like proteins in Borrelia have TM spanning regions of similar length to those present in ...these proteins, while similar in structure to ...OmpA-like proteins and as such it is not guaranteed ...

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Identification and Structural Characterisation of Novel Outer Membrane Proteins in the genus Borrelia

Identification and Structural Characterisation of Novel Outer Membrane Proteins in the genus Borrelia

... OmpA-like proteins in Borrelia have TM spanning regions of similar length to those present in ...these proteins, while similar in structure to ...OmpA-like proteins and as such it is not guaranteed ...

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Identification of Campylobacter jejuni and C  coli by gel electrophoresis of the outer membrane proteins

Identification of Campylobacter jejuni and C coli by gel electrophoresis of the outer membrane proteins

... Analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate Campylobacterjejuni (16 strains) from Campylobacter coli (10 strains).. This observ[r] ...

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