expression plasmid
Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT dependent protein production by gag encoded RNA
... (Figure 7), the effect of SRp20 overexpression on gag mRNA association with polysomes was assessed. HEK293T cells were co-transfected with the gag expression plasmid and either the SRp20 wild-type, ...
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Ebola Virus VP24 Binds Karyopherin α1 and Blocks STAT1 Nuclear Accumulation
... Western blots and coimmunoprecipitations. To detect STAT1 levels, 293T cells were transfected as described above with 10 ng of a human IRF-9 expression plasmid (pCAGGS-hIRF-9) and 2.5 g of the ...
12
Viral Interferon Regulatory Factor 1 of Kaposi's Sarcoma-Associated Herpesvirus Binds to p53 and Represses p53-Dependent Transcription and Apoptosis
... (no expression plasmid); right top, pcDNA3/HA-p53 (1 g) plus pcDNA3 (1 g); bottom left, pcDNA3/HA-p53 (1 g) plus pcDNA3/vIRF1 (1 g); bottom right, pcDNA3/vIRF1 (1 g) plus pcDNA3 (1 ...
6
Roles of the Envelope Proteins in the Amplification of Covalently Closed Circular DNA and Completion of Synthesis of the Plus-Strand DNA in Hepatitis B Virus
... the expression plasmid for WT HBV (TL25), in which expression of different combinations of the envelope proteins were ...the expression of the pgRNA as well as the template for the ...
12
Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 K-bZIP Modulates Latency-Associated Nuclear Protein-Mediated Suppression of Lytic Origin-Dependent DNA Synthesis
... TR plasmid (latent ...LANA expression on lytic repli- ...the plasmid containing the TR of HHV8 to the transfection ...this plasmid to the cotransfection mixture had no apparent effect on lytic ...
10
Disruption of Type I Interferon Signaling by the Nonstructural Protein of Severe Fever with Thrombocytopenia Syndrome Virus via the Hijacking of STAT2 and STAT1 into Inclusion Bodies
... control plasmid (vector) or the plasmid encoding NSs were treated with IFN- ␣ or left untreated for 30 min, and then cell lysates were subjected to Western blot ...NSs expression plasmid ...
10
Gene transfer into the airway epithelium of animals by targeting the polymeric immunoglobulin receptor
... irrelevant plasmid (pCMV lacZ) bound to the bona fide carrier or the expression plasmid (pGEMluc) bound to a carrier based on an irrelevant Fab fragment resulted in background levels of luciferase ...
11
Adenovirus VA1 Noncoding RNA Can Inhibit Small Interfering RNA and MicroRNA Biogenesis
... of expression of VA1 in adenovirus-infected cells, it is possible that VA1, which is known to bind Exp5 specifically (12), simply outcompetes pre-miRNAs and shRNAs for a lim- ited pool of ...of expression ...
9
EXPRESSION OF NONSTRUCTURAL GLYCOPROTEIN NSP4 OF SAIl SIMIAN ROTAV IRUS IN ESCHERICHIA COLI AND PRODUCTION OF ANTIBODY AGAINST IT
... into plasmid vectors. The recombinant plasmid was characterized by re striction enzyme and ...of expression plasmid containing nsp4 gene was performed and expression of NSP4 was ...
8
Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid
... the expression cassette PlipB1T and PlipB3T into the expression plasmid pELipAB a ...independent expression cassettes. Each expression cassette contained the complete set of the T7 ...
12
Inducible MicroRNA-3570 Feedback Inhibits the RIG-I-Dependent Innate Immune Response to Rhabdovirus in Teleost Fish by Targeting MAVS/IPS-1
... miR-3570 expression profile in miiuy croaker (Miichthys miiuy) macrophages during SCRV infection and found that miR-3570 was upregulated upon SCRV ...MAVS expression, which eventually repressed the type I ...
16
Capsid Protein of Eastern Equine Encephalitis Virus Inhibits Host Cell Gene Expression
... the expression of reporter genes driven by RNA polymerase II but not by the T7 reporter construct, suggesting a mechanism whereby the capsid targets some aspect of RNA polymerase II synthesis or induces host cell ...
11
TSPAN12 is overexpressed in NSCLC via p53 inhibition and promotes NSCLC cell growth in vitro and in vivo
... TSPAN12 expression, which is frequently upregulated in NSCLC, is inversely correlated with p53 ...the expression levels of TSPAN12 were also increased in three human NSCLC cell lines compared to human ...
9
<p>Gluconokinase IDNK Promotes Cell Proliferation and Inhibits Apoptosis in Hepatocellular Carcinoma</p>
... Our microarray data suggested that tRNA charging was inhibited by the deregulated expression of IDNK in HCC cells. tRNAs are noncoding RNAs that play a key role in protein translation for cancer cell ...
10
A common variant alters SCN5A–miR 24 interaction and associates with heart failure mortality
... MiRs have been established as key effectors in cardiovascu- lar biology and disease (25–27). These short RNAs are incorpo- rated into Argonaute (Ago) proteins, producing effector complexes capable of base-pairing with ...
11
Ros, Francesca (2001): Regulation of maize Ac/Ds transposition by replication and DNA methylation. Dissertation, LMU München: Fakultät für Biologie
... EI plasmid digestion products appear before and after ...undigested plasmid is visualized anymore by Southern ...untransfected plasmid, they are independent of any host de novo methylation ...
123
DNA Topoisomerase 1 Facilitates the Transcription and Replication of the Ebola Virus Genome
... a plasmid for the expression of a virus-like RNA encoding firefly luciferase and with plasmids for the expression of viral proteins responsible for the transcription and replication of the virus-like ...
8
Activation of Heterologous Gene Expression by the Large Isoform of Hepatitis Delta Antigen
... Effect of LHDAg on various promoters. To assess the range of potential transcription factor targets of LHDAg, we tested two series of promoters in transient transfection assays. The hsp70 minimal promoter and the TATA ...
8
Modulation of anti-cancer drug sensitivity through the regulation of mitochondrial activity by adenylate kinase 4
... CKMT1 expression (Table 3), which is known to form complexes with VDAC, ANT and other substances in the mitochondrial intermembrane space [37], suggesting a compensational response to insufficient ADP recycling ...
15
Molecular analysis of Shigella flexneri bacteriophage SfV BamHI fragment A encoding the viral late region and the role of SfV genome in host virulence
... Plasmid-encoded Virulence Factors Shigella carry a large 220 kb virulence plasmid containing a 30 kb sequence [Maurelli, 1985 #343] that encodes genes essential in the expression of inva[r] ...
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