FIBRONECTIN-BINDING PROTEIN
Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin Binding Protein
... human fibronectin, as previously described ...human fibronectin (lanes 2 to 4), whereas the 26-kDa GST produced by ...bind fibronectin (arrow, lane 1). This indicates that the binding of ...
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Fibronectin Binding Protein Gene Recombination and Horizontal Transfer between Group A and G Streptococci
... M protein (emm) (5, 24, 26), streptococcal pyrogenic exotoxin G (SpeG) (23), streptolysin O, and streptokinase in GGS is consistent with the observation that these two bacteria have a common disease ...tococcal ...
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Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin binding adhesins fibronectin binding protein A and fibronectin binding protein B from Staphylococcus aureus
... fibronectin-binding protein A is organized into 11 tandem repeats, six of which bind the ligand with high affinity; other sites bind more ...weakly. Fibronectin-binding protein B has been ...
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Evolution of sfbI Encoding Streptococcal Fibronectin Binding Protein I: Horizontal Genetic Transfer and Gene Mosaic Structure
... Streptococcal fibronectin-binding protein I (SfbI), also known as protein F1, is probably the most extensively charac- terized fibronectin-binding protein in group A ...
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Staphylococcus aureus Fibronectin Binding Protein A Mediates Cell Cell Adhesion through Low Affinity Homophilic Bonds
... surface-located fibronectin-binding protein A (FnBPA) plays an important role in the accumulation phase of biofilm formation by methicillin-resistant ...Low-affinity binding by means of FnBPA ...
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Human Immunoglobulin G Cannot Inhibit Fibrinogen Binding by the Genetically Diverse A Domain of Staphylococcus aureus Fibronectin-Binding Protein A
... Measurement of antibodies. IgG levels against all recombinant N2N3 A domains of FnBPA isotypes in the serum samples of bacteremia patients were measured using a bead-based flow cytometry technique (xMAP; Luminex ...
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Pneumocystis carinii attachment to cultured lung cells by pneumocystis gp 120, a fibronectin binding protein
... significant decrease in attachment of P. carinii to A549 cells from 44.2 +/- 5.5% to 22.4 +/- 4.2% (P less than 0.01). Preincubation of the P. carinii organisms with a polyclonal antibody to gp120 also resulted in a ...
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Subdomains N2N3 of Fibronectin Binding Protein A Mediate Staphylococcus aureus Biofilm Formation and Adherence to Fibrinogen Using Distinct Mechanisms
... with plasmids pFnBA4 and pFnBB4 restored expression of FnBPA and FnBPB, respectively (Fig. 2C). BH1CC grown to stationary phase bound to fibronectin, but BH1CC ⌬ fnbAfnbB and station- ary-phase SH1000 did not ...
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Evaluation of RevA, a Fibronectin-Binding Protein of Borrelia burgdorferi, as a Potential Vaccine Candidate for Lyme Disease
... IL). Protein purity was assessed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electro- phoresis followed by staining with Coomassie brilliant blue (see ...shown). Protein concentrations were ...
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Fibronectin binding protein B (FnBPB) from Staphylococcus aureus protects against the antimicrobial activity of histones
... CWA protein, FnBPB, was responsible for this ...FnBPB binding to individual histones was tested, it was found that all types could bind but that H3 displayed the highest binding and bactericidal ...
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Prevalence and distribution of adhesins and the expression of fibronectin binding protein (FnbA and FnbB) among Staphylococcus aureus isolates from Shahrekord Hospitals
... Staphylococcusaureus is an important hospital pathogen, the most common cause of surgical wound infections, the second leading cause of hospital pneumonia, and also responsible for 35% of hospital-acquired bacteremia ...
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The Borrelial Fibronectin-Binding Protein RevA Is an Early Antigen of Human Lyme Disease
... above). Binding of specific antibodies was detected by using alkaline phos- phatase-conjugated goat anti-human IgG serum (1:100; Genzyme Virotech) or goat anti-mouse Ig antibodies (1:10,000; GE Healthcare), as ...
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Sequence diversity in the A domain of Staphylococcus aureus fibronectin binding protein A
... Schematic representation of FnBPA from S. aureus 8325-4. (a) The N-terminus of FnBPA contains a signal sequence (S) followed by a 475 amino acid A domain, part of which is the binding site for fibrinogen and ...
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Fibronectin binding protein B variation in Staphylococcus aureus
... Ligand binding by recombinant FnBPB N23 isotypes was compared by ELISA-based solid phase assays and surface plasmon ...and fibronectin dose-dependently and saturably with similar ...affinities. ...
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Surface displaced alfa-enolase of Lactobacillus plantarum is a fibronectin binding protein
... involved in the interaction vary mostly among different species and strains, mainly regarding interaction between bacterial adhesins and extracellular matrix (ECM) or mucus proteins [19,20]. Adhesion of L. plantarum to ...
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Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection
... surface protein (esp), collagen-binding-protein (ace) and PavA-like fibronectin-binding protein (efbA), could facilitate initial colonization, biofilm formation, destruction of ...
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Potential diagnostic biomarkers for chronic kidney disease of unknown etiology (CKDu) in Sri Lanka: a pilot study
... FN1: Fibronectin-1; G6PD: Glucose-6-phosphate dehydrogenase; GCLC: Glutamate cysteine ligase C subunit; GFR: Glomerular filtration rate; GH: Girandurukotte Healthy; GSH: Glutathione; GSTM1: Glutathione-S- ...
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Fibronectin binding by Salmonella strains: evaluation of a particle agglutination assay
... Thirty-five Salmonella strains isolated from human cases of salmonellosis were tested and compared for their fibronectin fn binding capacities by using two fn-particle agglutination assa[r] ...
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A novel single amino acid deletion impairs fibronectin function and causes familial glomerulopathy with fibronectin deposits: case report of a family
... MLGRM: performed histological diagnosis and had a major contribution for writing the manuscript. FBC: recruited subjects, gathered patients ’ histories, performed renal biopsies and followed patients after the diagnosis. ...
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Microtubule associated protein 1 light chain 3 is a fibronectin mRNA binding protein linked to mRNA translation in lamb vascular smooth muscle cells
... of fibronectin mRNA trans- ...by fibronectin 3 9 UTR bearing intact or mu- tated ...lower fibronectin–producing aorta cells ...endogenous fibronectin mRNA translation. We next purified a 15-kD ...
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