Fluorescence anisotropy
Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... The affinity of the origin-binding domain (OBD) of simian virus 40 large T antigen for its cognate origin was measured at equilibrium using a DNA binding assay based on fluorescence anisotropy. At a ...
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Characterization of the Minimal DNA Binding Domain of the Human Papillomavirus E1 Helicase: Fluorescence Anisotropy Studies and Characterization of a Dimerization-Defective Mutant Protein
... in anisotropy was lower for the probe with only the proximal E1BS than for one with two E1BS is consistent with the binding of a single DBD molecule, rather than two, to this mutant ...in anisotropy de- ...
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Probing beta amyloid aggregation using fluorescence anisotropy : experiments and simulation
... using fluorescence anisotropy, a technique that provides information on the rotational diffusion of the fluorescing tyrosine (Tyr) side ...yield anisotropy in line with the experimental ...
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Fluorescence anisotropy of rhodamine 6G using incoherent laser light
... The top graph of figure (9) shows the parallel and perpendicular fluorescence decays measured using incoherent fluorescence upconversion experiment with background removed, and the middl[r] ...
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Single and two photon time resolved polarised fluorescence studies of probe molecule dynamics in nematic liquid crystals
... photon fluorescence anisotropy decays for E=0° and 90° are shown in figure ...(corrected) fluorescence anisotropy was observed to yield steady state probe alignment of ...E=0° ...
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Single and three pulse photoselection of dye probes in nematic liquid crystals
... induced fluorescence 7-9 and surface second harmonic generation 10 ...Picosecond fluorescence anisotropy studies of molecular probes have been shown to yield useful information on molecular order, ...
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Synthesis of Highly Functionalized Oligobenzamide Proteomimetic Foldamers by Late Stage Introduction of Sensitive Groups
... Biophysical analyses Using a previously described fluorescence anisotropy competitive HIF-1α/p300 assay,10,31 the helix 2 mimetic 5 was shown to be ineffective as a HIF-1α/p300 inhibitor;[r] ...
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Domain Organization of Vaccinia Virus Helicase-Primase D5
... Fluorescence anisotropy as well as SPR measurements using the monomeric helicase construct D5381–785 or the primase domain D51–335 did not indicate any binding under our experimental con[r] ...
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Overcoming the aggregation problem : a new type of fluorescent ligand for ConA-based glucose sensing
... Fluorescence anisotropy was used to determine the binding affinity between APTS-MT and native ConA in TRIS buffer at 22 deg ...stead-state anisotropy from the solution. Assuming the change in ...
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The Leishmania PABP1–eIF4E4 interface : a novel 5′–3′ interaction architecture for trans spliced mRNAs
... with fluorescence anisotropy data described in the next section, this indicates that, like eIF4E proteins from other organisms, Leishmania eIF4E4 has a dorsal binding domain structure for binding to the N- ...
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Inhibition of human factor VIIIa by anti A2 subunit antibodies
... in fluorescence anisotropy that results from the binding of Factor VIIIa to fluorescein-5-maleimidyl-D-phenylalanyl-prolyl-arginyl-FIXa (Fl-M-FPR-FIXa) ...
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Prediction of Neutron Emission Anisotropy for Validation of an Analysis Model for Neutron Spectra in Beam-Injected LHD Deuterium Plasmas
... emitted in the directions χ = 0 ◦ and 180 ◦ are increased, and those emitted in the direction χ = 90 ◦ are decreased, as shown in Fig. 4. Therefore, in this case, the fraction of the neutron flux at θ = 0 ◦ increases and ...
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Can Diffusion Tensor Imaging Noninvasively Detect IDH1 Gene Mutations in Astrogliomas? A Retrospective Study of 112 Cases
... One case in group 2 was recurrent glioma. The differences in age and sex between groups 1 and 2 were not statistically significant (Table 1). The maximal FA (0.25 ⫾ 0.16) and rmFA (0.35 ⫾ 0.22) values in group 2 were ...
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Estimation of the Effect of Anisotropy on Young’s Moduli and Poisson’s Ratios of Sedimentary Rocks Using Core Samples in Western and Central Part of Tripura, India
... of anisotropy are very useful for correlation of sonic logs in vertical and deviated wells and for borehole sur- face seismic imaging and studies of amplitude variation with offset ...permeability ...
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Numerical anisotropy in finite differencing
... ical anisotropy, by applying various ...numerical anisotropy of finite dif- ference schemes applied to transmission-line modeling (TLM) ...introduce anisotropy into the disper- sion characteristics of ...
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Anisotropy and nonlinearity in superlattices II
... the anisotropy, the z-direction electron and hole masses become too different, with a much larger increase in hole ...and anisotropy parameter in ...
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Magnetic resonance of the NiFe2O4 nanoparticles in the gigahertz range
... more, the medium binding energy component (Ob) located at 531.2 ± 0.2 eV (Oc) is associated with the O 2− ions in the oxygen-deficient regions (O vacancies) [30]. The result obviously demonstrates the presence of oxygen ...
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Study of Magnetization Switching in Coupled Magnetic Nanostructured Systems
... magnetic anisotropy of the MRAM element. The toggle switching is achieved using a suitable time sequence for word and bit fields which rotate the effective magnetic moment vector of the MRAM cell, as described in ...
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Dielectric permittivity modulation of liquid crystals for microwave applications
... In summary, we have measured dielectric properties, in the region from 0.01GHz up to 6GHz, of nematic liquid crystals with a range of birefringence values. At 3GHz, the maximum dielectric modulation was found to be 22.5% ...
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On the stability of low anisotropy dendrites,
... a front into the Mullins-Sekerka unstable needle crystal profile, leads one to expect sidebranches to encroach on the tip, at least transiently, in the limit of small kinetic or surface.[r] ...
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