Fluorescence lifetime-based thermometer connected with a PC
Ultra portable explosives sensor based on a CMOS fluorescence lifetime analysis micro system
... the fluorescence lifetime before and after the 15-minute ...the fluorescence lifetime, clean nitrogen with a similar flow-rate to that used in the exposure process, was flowed through the ...
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GPU acceleration of time-domain fluorescence lifetime imaging
... (PMT) based TCSPC instru- ments are capable of single-photon detection, and they can have multiple channels to boost the ...systems, lifetime analysis has become a bottleneck for real-time FLIM ...
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Development and biomedical application of fluorescence lifetime imaging endoscopes
... bundle based system applied to tissue demonstrated by McGinty et al 183,184 was based on the time-gated imaging technique described in 2,182 and used a mode- locked laser (355 nm) generating ~ 100 ps pulses ...
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Time-domain measurement of fluorescence lifetime variation with ph.
... of lifetime change with pH variation. Most dyes had a lifetime change of less than one nanosecond over the crucial physiological range of ...potential lifetime based pH ...largest ...
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A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein
... Several receptors for glucose have been investigated for fluo- rescence-based glucose sensing 4 including boronic acid deriva- tives, 4 concanavalin A 4,17,19,21 and other lectins, 8 glucose oxidase 4,13,16 and ...
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Optimizing Laguerre expansion based deconvolution methods for analysing bi-exponential fluorescence lifetime images
... require shinning pulsed lasers with a low duty-cycle, reducing the efficiency of photon collection. There are two major contributions in this paper. First, we introduce new criteria for selecting LBFs according to the ...
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Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy.
... Photon counting approaches are particularly useful for fluorescence microscopy methods employing a camera to enable TCSPC-based FLIM, the FLIM method with the highest signal-to-noise ratio. The extremely ...
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Optimizing Laguerre expansion based deconvolution methods for analysing bi-exponential fluorescence lifetime images
... require shinning pulsed lasers with a low duty-cycle, reducing the efficiency of photon collection. There are two major contributions in this paper. First, we introduce new criteria for selecting LBFs according to the ...
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Build Instructions for TempBug: Internet-Connected Thermometer Step 1: Gather the parts
... Imp firmware is written in an object-oriented language called Squirrel, which looks and feels a lot like JavaScript. Squirrel runs in a virtual machine on top of the impOS inside the Imp, so runtime errors won't knock ...
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A CMOS time-resolved fluorescence lifetime analysis micro-system
... A CMOS time-resolved analysis system has been designed, consisting of a fully addressable array of 16 × 4 array of SPADs integrated with on-chip signal processing and timing circuits. Each pixel measured 100 μm × 200 μm. ...
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Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager
... of lifetime estimation both in terms of speed and data ...FLIM based DNA microarray readout and the recording of arrival time histograms confirming multi- exponential ...and fluorescence ...
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Machine learning for faster and smarter fluorescence lifetime imaging microscopy
... limit fluorescence lifetime measurement accuracy by reducing the ‘photon economy’ ...the lifetime is an intrinsic property of fluorescence molecules, FLIM is capable of separating fluorophores ...
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Monitoring Biosensor Activity in Living Cells with Fluorescence Lifetime Imaging Microscopy
... Abstract: Live-cell microscopy is now routinely used to monitor the activities of the genetically encoded biosensor proteins that are designed to directly measure specific cell signaling events inside cells, tissues, or ...
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Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors
... the fluorescence lifetime of tumor and contralateral sites were measured in ...time based on our measured fluorescence intensity at the tumor and contra-lateral sites at different time points ...
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DEVELOPMENT OF A MULTIPLEXED CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPE FOR SCREENING APPLICATIONS
... Chapter 1. Introduction 1.1 Motivation Proteins have critical roles within the cell, often interacting in large networks or pathways to carry out a biological function. At one point in time, scientists believed it would ...
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Detection of In vivo Oral Epithelial Cancer using Fluorescence Lifetime Imaging
... 28 Table 6 represents the LOO sample-level confusion matrix of the entire area, indicating that all samples were classified correctly, except for one dysplasia. Figure 12 Scatter plot based on total training ...
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Towards unsupervised fluorescence lifetime imaging using low dimensional variable projection
... To further guarantee unsupervised analysis, we present a simple lifetime extraction method based on a classical algorithm called the variable projection method (VPM) [43, 45]. VPM is a very useful tool for ...
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Fluorescent multiple chemical sensing using time-domain fluorescence lifetime imaging
... long fluorescence lifetime (here simply referred to as luminescence, covering all kinds of molecular light emission), short-lived fluorescent background can be surpressed in the time ...all ...
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Fluorescence Lifetime Imaging Microscopy (FLIM) System for Imaging of Oral Cancer and Precancer
... and lifetime images. Additionally, the spectral and lifetime data of the DMBA-treated pouch is different than the normal control pouch, indicating the ability to detect precancerous ...average ...
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PC Thermometer
... vii ABSTRACT This project is to design and develop a device that can be used to measure temperature inside the computer’s CPU. This project also can be designed for measuring temperature outside the computer’s CPU. This ...
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