fluorescence lifetime imaging and microscopy
Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... steady-state fluorescence intensity still suffered from artifacts arising from the heterogeneity of tissue optical properties [8], local environment, unknown chromophores with over- lapped spectra, and the ...
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Mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy
... scanning microscopy (CLSM) and fluorescence lifetime imaging microscopy (FLIM) are able to provide important information about the concen- tration and the spatial distribution of a ...
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Direct Interaction of Baculovirus Capsid Proteins VP39 and EXON0 with Kinesin-1 in Insect Cells Determined by Fluorescence Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy
... scanning microscopy (CLSM) as previously ...Two-photon fluorescence lifetime imaging analysis of transfected insect ...Two-photon-induced fluorescence lifetime images were ob- ...
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Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells
... Results: Fluorescence lifetime imaging microscopy (FLIM) can be is used to determine intra- or intermolecular interactions by fluorescently labeling epitopes on one or two different ...
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A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein
... beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the ...fibre. Fluorescence lifetime responses ...
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GPU acceleration of time-domain fluorescence lifetime imaging
... Abstract. Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chem- istry, and medical ...up lifetime calculations compared to CPU-OpenMP ...
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Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells
... Fluorescence lifetime imaging microscopy (FLIM) was performed on Zeiss LSM 710 microscope (Carl Zeiss, Jena, Germany) using a ×40 water immersion objective, ...for Fluorescence Dynamics ...
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HIV-1 Vpr Oligomerization but Not That of Gag Directs the Interaction between Vpr and Gag
... developed fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer-based assays to monitor the interaction between Pr55 Gag and Vpr in HeLa ...
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Micro or nanorod and nanosphere structures derived from a series of phenyl porphyrins
... force microscopy (AFM) studies were performed using an Explorer ...AFM. Fluorescence microscopy studies were performed using a Zeiss inverted microscope system coupled with a 532 nm excitation ...
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Novel Bioluminescent Activatable Reporter for Src Tyrosine Kinase Activity in Living Mice
... Molecular imaging, especially optical imag- ing, provides a new platform for noninvasive visual- ization of biological processes at the molecular level in ...Optical imaging, including fluorescence ...
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Apertureless near field optical microscopy for fluorescence imaging
... effects. Fluorescence quen- ching could be identified by taking fluorescence lifetime measurements using a pulsed laser and measuring the delay between a laser pulse and the fluorescent photons ...
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Luminescence lifetime imaging microscopy by confocal pinhole shifting (LLIM-CPS)
... Initially, fluorescence of TMRM increased slightly after 2 min, perhaps due to continued loading into mitochondria (Figure ...TMRM fluorescence was lost, indicating depolarization of ...
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Bright Field and Fluorescence Chip Scale Microscopy for Biological Imaging
... Figure 3 - 1. Schematic diagram of ePetri device for SPSM imaging of motile microorganisms. (a) ePetri dish imaging platform is composed of an ePetri chip, camera module and a control computer. A Peltier ...
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Single-shot time-gated fluorescence lifetime imaging using three-frame images
... the fluorescence lifetime images with a single excitation to freeze the instantaneous ...the fluorescence delay functions by gating the image intensifier of an ICCD camera with different ...
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Recent advances in hydrogen peroxide imaging for biological applications
... particular, several mitochondria-targeted probes have been generated, including Mitochondrial Peroxy Yellow 1 (MitoPY1) and SHP-Mito [25,42,43,51], which utilize a positively charged phosphonium moiety for mitochondrial ...
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Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... same fluorescence lifetime, both in the nucleoplasm and in the nuclear ...uniform fluorescence lifetime values across the cell nucleus, we found that the lifetime of fibrillarin-GFP ...
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"Handbook of biomedical optics", edited by David A. Boas, Constantinos Pitris, and Nimmi Ramanujam
... and imaging agents, but fluorescence-based visualization of fast changing myocardial transmembrane potentials seem to have been ...Light Imaging of Biological Tissues), or a separate chapter ...
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Using in vivo fluorescence lifetime imaging to detect HER2-positive tumors
... Fluorescence lifetime imaging is based on evaluation of the average time that electronically excited fluoro- phore stays in that excited state before its transition to a ground state, accompanied by ...
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TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2
... cell imaging: It is a photophysical property of the probe that is relatively in- dependent of the ascertainment platform and indifferent to ...TriPer’s lifetime indicated that it is found in a redox state ...
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A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging
... generated fluorescence in the microscope focal plane via a two photon absorption process and was back-projected through the system to the detection ...each fluorescence image of the beamlet array was ...
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