Fluorescence Polarization
Fluorescence polarization studies of rat intestinal microvillus membranes
... of fluorescence polarization of diphenylhexatriene, which provides an index of the "microviscosity" of the lipid regions of the membrane, is exceptionally high in microvillus membranes, the highest ...
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Fluorescence Polarization Assay for Detection of Brucella abortus Antibodies in Bulk Tank Bovine Milk Samples
... ish color (optical properties as reported by the Dairy Science and Technology of the University of Guelph [http://www.food- sci.uoguelph.ca/dairyedu/chem.html]) to the whey, again prob- ably due riboflavin ...
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Use of Recombinant Flagellin Protein as a Tracer Antigen in a Fluorescence Polarization Assay for Diagnosis of Leptospirosis
... The objective of the present study was to investigate the usefulness of a recombinant flagellar protein, FlaB, of Leptospira interrogans serovar pomona in the serodiagnosis of leptospirosis by the fluorescence ...
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Determining a Diagnostic Cut Off on Fluorescence Polarization Assay (FPA) for Bovine Brucellosis in Carchi, Ecuador
... through fluorescence polarization assay (FPA) in Carchi—Ecuador was ...Fluoresce Polarization Assay (FPA) and competitive enzyme-linked inmunosorbent assay (cELISA) as confirmatory test, and then ...
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Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.
... of fluorescence polarization described by Perrin ...cence polarization has been widely applied to the study of molecular interactions in a variety of biological systems (6) and to the determination ...
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A simple approach for rapid and cost-effective quantification of extracellular vesicles using a fluorescence polarization technique
... the fluorescence polarization (FP) detection of lipophilic fluorescein probe, 5-dodecanoylamino fluorescein (C12-FAM), which has been used for the determination of critical micelle concentration value of ...
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Mapping the N-Terminal Residues of Epstein-Barr Virus gp42 That Bind gH/gL by Using Fluorescence Polarization and Cell-Based Fusion Assays
... from fluorescence polarization, cell-cell fusion, and viral infection assays demonstrated that 33 residues corresponding to 44 to 61 and 67 to 81 of gp42 were indis- pensable for maintaining ...
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Detection of HBV Genotypes of Tumor Tissues and Serum by A Fluorescence Polarization Assay in North Western China's Hepatocellular Carcinoma Patients
... Advances in molecular biotechnology have allowed the detailed study of the viral genotypes of HBV and the occult HBV infections. Numerous studies have been done on investigating the distribution and the impact of HBV ...
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Assessment of Fluorescence Polarization Titres as a Prediction Indicator of Which Brucella Spp. Circulate in a Domestic Ruminant Animal Populations
... Brucellosis is an important zoonotic bacterial disease and has been an emerging disease since the discovery of Brucella melitensis by David Bruce in 1887. Eradication programmes date back to 1905, based on vaccination, ...
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Development of a Fluorescence Polarization Based Diagnostic Assay for Equine Infectious Anemia Virus
... The control of equine infectious anemia virus (EIAV) infections of horses has been over the past 20 years based primarily on the identification and elimination of seropositive horses, predominantly by a standardized agar ...
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Measurement of Fluorescence Polarization Angle for Characterization of Canola Oils
... of polarization can be assigned [11] as = nat + elec, where nat is natural polarization angle due to asymmetric TG molecules, or so called as optically active TG molecules, and elec is ...
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Laboratory evaluation of five assay methods for vancomycin: bioassay, high pressure liquid chromatography, fluorescence polarization immunoassay, radioimmunoassay, and fluorescence immunoassay
... We compared the precision and accuracy of five methods used to measure the concentration of vancomycin in bioassay, high-pressure liquid chromatography, fluorescence polarization immunoa[r] ...
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Amniotic Fluid Microviscosity Determined by Fluorescence Polarization: Methodology and Relation to Gestational Age
... Because there were important differ- ences in the period of gestation during which sequential samples were obtained, the data from individual pregnant women were grouped as follows: grou[r] ...
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Performance characteristics between TDx®FLx and TBA™-25FR for the therapeutic drug monitoring of methotrexate
... The time calibration curve showed excellent linearity, with correlation coefficients generally ≥ 0.99. The cali- bration curve spanned one order of magnitude with a linear working range from the lowest to the highest ...
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How to Make a Mudsparkler
... Because the length of guest 3 exceeds the diameter of the 1D channels in the host, we expect that a limited range of orientations is accessible to the guest, within which rotational diffusion may occur. The distribution ...
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Evaluation of quantitative assays for the identification of direct signal transducer and activator of transcription 3 (STAT3) inhibitors
... vitro fluorescence polarization assay, using recombinant STAT3 protein, has successfully identified compounds that target the SH2 domain; however, no assay has been reported to identify inhibitors that bind ...
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An Irreversible Inhibitor of HSP72 that Unexpectedly Targets Lysine-56.
... To establish whether our rationally designed targeted covalent inhibitor 8 could form a reversible complex, its affinity was assessed using a fluorescence polarization (FP) assay to meas[r] ...
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Dexamethasone treatment alters kinetics properties of liver mitochondrial F0 F1 ATPase and membrane lipid profiles in developing and adult rats
... of fluorescence polarization, samples were taken in 3 ml of buffered sucrose solution ...branes. Fluorescence polarization was measured in a Shimadzu RF 5000 spectrophotofluorimeter with a po- ...
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Polarization microscopy for visualizing FRET between identical fluorophores : monitoring receptor clustering in live cells
... The fluorescence polarization microscope was successfully implemented in the existing fluorescence microscopy ...the polarization microscope the anisotropy of enhanced green fluorescent ...
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Effect of essential fatty acids on glucose-induced cytotoxicity to retinal vascular endothelial cells
... enhanced fluorescence polarization and microviscocity that reverted to normal by treatment with Zn 2+ and ...enhanced fluorescence polar- ization and microviscocity changes induced by ...to ...
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