Fluorescent Staining and Microscopy
<p>Evaluation Of The Efficacy Of Fluorescent Staining And Chicago Sky Blue Staining As Methods For Diagnosis Of Dermatophytosis In Hair And Nails</p>
... diagnostic microscopy methods, including Chicago sky blue and Calco fl uor white fl uorescent staining, and com- pare them with traditional KOH wet mounting and culture techniques for the diagnosis of fungi ...
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Comparison of Sensitivity and Specificity of ZN and Fluorescent Stain Microscopy with Culture as Gold Standard
... that fluorescent staining of smears increases sensitivity of direct microscopy; so ZN staining is being replaced with fluo- rescent microscopy in RNTCP in ...of microscopy for ...
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Improved sensitivity of direct microscopy for Mycobacterium tuberculosis in lymph nodes: comparison of fluorescent microscopy and modified bleach method ZN microscopy along with conventional ZN staining method
... staining method Gul Aalmeen, Sangeeta Sharma*, Sneha Vishwasrao, Rani Bansal, Anjali Khare INTRODUCTION Lymphadenopathy is the most common presentation of extra pulmonary tuberculosis (EPTB). Tuberculous ...
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Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging
... Therefore in all our Single colour experiments the exposure time was between 3 to 10 and the %T was in the range of 10 to 50. The Colocalization experiments were performed with ER Tracker Green. The Dual colour ...
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Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo
... This workflow delivers several advances in super-resolution imaging. The overlaid SR-EM images provide a new ‘ground truth’ method for testing super-resolution reconstruction algorithms and parameters. For example, we ...
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Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
... Critical steps within the protocol This protocol describes the ability to monitor mitochondria calcium accumulation and ΔΨ m simultaneously with Fluo-4, AM and TMRM. During the staining process it is critical to ...
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Light sheet fluorescent microscopy versus confocal microscopy: in quest of a suitable tool to assess drug and nanomedicine penetration into multicellular tumor spheroids
... Overall these results confirmed the penetration capacity of small molecules into large spheroids and data were in agreement with the penetration depth demonstrated by other advanced microscopic systems [16, 49]. Fig. 6. ...
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Comparison of Light-Emitting Diode Fluorescent Microscopy Against Bright-Field Microscopy and JD-TB-Antigen Test for the Diagnosis of Pulmonary Tuberculosis
... Two sets of direct smears were prepared from each sputum sample before concentration by using NACL-NaOH taking a small portion of sputum using applicator stick and evenly distributed smear were made approximately over an ...
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Comparitive evaluation of loop mediated isothermal amplification in the diagnosis of mycobacterium tuberculosis with culture and fluorescent microscopy.
... By eliminating the use of costly thermocyclers this technique promises a great and low cost alternative in diagnosing infectious diseases. The laboratory diagnostic method include Ziehl-Neelson acid fast staining ...
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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins
... SR microscopy and EM 23–26 ...and staining protocols for EM are often not compatible with fluores- cence 21,27 ...heavy-metal staining instead of directly imaging biomaterial, as is done with ...
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Light Emitting Diode (LED) based Fluorescent Microscopy versus Bright Field Microscopy for the Diagnosis of Tuberculosis from Extrapulmonary and Non-sputum Pulmonary Samples
... Abstract Microscopy is the mainstay in the diagnosis of tuberculosis (TB) in resource poor ...Auramine-O staining and LED (AO-LED) microscopy for the diagnosis of tuberculosis from extrapulmonary and ...
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Post-diaminobenzidine Treatments for Double Stainings: Extension of Sulfide-Silver-Gold Intensification for Light and Fluorescent Microscopy
... Silver intensification techniques use the catalytic property of the DAB polymer to reduce silver ions to fine metal grains by formaldehyde 8 or ascorbic acid. 9 The atomic silver is then usually substituted with gold in ...
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Methods of staining and visualization of sphingolipid enriched and non-enriched plasma membrane regions of Arabidopsis thaliana with fluorescent dyes and lipid analogues
... artifacts, and detergent resistance does not necessarily imply lipid rafts [6,7]. However, the enrichment of a spe- cific protein in DRMs suggests an affinity for a distinct lipid environment and indicates localization ...
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Purpose Of Staining Samples In Light Microscopy
... light microscopy is ...of staining samples in microscopy is never directly Vectorially using the purpose of staining in light in order to do not present in ...of staining samples in ...
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Negative Staining Protocol For Transmission Electron Microscopy
... for microscopy has attracted funding from the results from omegasomes in both advantages of leaf virus particles to the study membrane distortion ...in staining protocol for transmission electron ...
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Fluorescent treponemal antibody absorption double staining procedure
... The Ortholux II microscope equipped with a N filter cube for reading rhodamine fluorescence was used in establishing acceptability of the proposed FTA-ABS DS reference reagents.. We foun[r] ...
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Negative staining and image classification — powerful tools in modern electron microscopy
... Fig. 1: Images of the TfR-Tf complex obtained with different negative staining protocols. a and b : Images of an untilted (a) and a 60° tilted sample (b) prepared by the conventional negative staining ...
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Description of a polyvalent conjugate and a new serogroup of Bacteroides melaninogenicus by fluorescent antibody staining
... This polyvalent conjugate gave a positive fluorescent antibody (FA) stain with 49 strains of Bacteroides melaninogenicus representing serogroups A, B, and C.. When addi- tional strains ([r] ...
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CYTOCHEMICAL STUDIES WITH AFRICAN HORSE SICKNESS VIRUS BY FLUORESCENT MICROSCOPY (*)
... Pretreat- ment of the infected cells with RNase or DNase did not prevent the intranuclear particles to acquire the yellow-green color when subsequently stained wit[r] ...
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Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination
... The bright center in fig. 3(b) can be explained in terms of geometrical optics: when the light is focused on the center of the sphere, rays propagate along radial directions and reflect back. In order to see the rings ...
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