force spectroscopy
Single-molecule force spectroscopy studies of fibrin A-a polymerization interactions via the atomic force microscope
... applied force with laser tweezers, AFM has been utilized to investigate the mechanical behavior of fibrin(ogen) polymers upon ...applied force of extension was hypothesized to initiate unfolding of the ...
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Interaction of an anticancer peptide fragment of azurin with p53 and its isolated domains studied by atomic force spectroscopy
... a force–distance cycle carried out in the atomic force spectroscopy ...contact force upon the substrate, the approaching phase is stopped (point 3); the cantilever begins to retract, reaches ...
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Determination of the Molar Mass of Surface-Grafted Weak Polyelectrolyte Brushes using Force Spectroscopy
... by force spectroscopy refers to the end-to-end distance for the linear polymer chain, but the distance between the ends of two side-chains when a multi-arm polymer is being ...
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Determination of the molar mass of surface-grafted weak polyelectrolyte brushes using force spectroscopy
... single-molecule force spectroscopy (SMFS) and shown to be consistent with results from gel permeation chromatography for the same comb polymer in aqueous ...atomic force microscope (AFM) tip can ...
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MDM2–MDM4 molecular interaction investigated by atomic force spectroscopy and surface plasmon resonance
... Abstract: Murine double minute 2 (MDM2) and 4 (MDM4) are known as the main negative regulators of p53, a tumor suppressor. They are able to form heterodimers that are much more effective in the downregulation of p53. ...
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Quantifying cellular mechanics and adhesion in renal tubular injury using single cell force spectroscopy
... applied force or stress and return to its original configuration when the stress is ...cell force spectroscopy (SCFS) to investigate the effects of early TGF-1 induced tubular injury on single cell ...
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Life in extreme environments: Single molecule force spectroscopy as a tool to explore proteins from extremophilic organisms
... molecule force spectroscopy to mechanically manipulate proteins from extremophilic organisms and the information which has been gained about their stability, flexibility and underlying energy ...
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Nickels, Philipp (2017): Force spectroscopy with DNA origami. Dissertation, LMU München: Fakultät für Physik
... Despite the great success, all three techniques suffer from two major limitations. The first limitation is the serial nature of conventional force spectroscopy. This serial nature leads to a low throughput ...
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Unraveling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy
... Acoustic Force Spectroscopy (AFS) is a single-molecule micromanipulation technique that uses sound waves to exert force on surface-tethered DNA molecules in a microfluidic ...from ...
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Single-molecule force spectroscopy on histone h4 tail cross-linked chromatin reveals fiber folding
... more gradual unstacking transition for 167-bp NRL MT fibers under reducing conditions (Fig. 4A). Likewise, sedimentation velocity analysis of 167- and 177-bp NRL MT fibers under reducing conditions indicated a reduced ...
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Klamecka, Kamila (2017): Single-molecule force spectroscopy of biological complexes. Dissertation, LMU München: Fakultät für Biologie
... before force spectroscopy was ...the force-distance curve when retracting the cantilever from the ...resulting force-distance profiles display a behavior not observed previously: the ...
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Ott, Wolfgang Bernhard (2017): Single molecule force spectroscopy with biological tools. Dissertation, LMU München: Fakultät für Physik
... Recently, experimental measurements were brought into prox- imity (Dong and Sahin, 2011; He et al., 2012; Schoeler et al., 2015) or even overlap (Rico et al., 2013) with all atom steered molecular dynamics (SMD) ...
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Opfer, Jan (2012): Single-molecule force spectroscopy studies of integrin-mediated cell signaling. Dissertation, LMU München: Fakultät für Physik
... ReNoiR, a recursive wavelet-based algorithm, aims at reducing the noise in order to make hidden features of the data visible without inducing disturbing artifacts. In contrast to kernel- or Fourier- based filters, the ...
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Co localised Raman and force spectroscopy reveal the roles of hydrogen bonds and π π interactions in defining the mechanical properties of diphenylalanine nano and micro tubes
... atomic force microscope (NanoWizard II, ...co-localised force spectroscopy and polarised Raman spectroscopy measurements for individual FF ...a force curve from a clean glass coverslip ...
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Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single cell force spectroscopy
... In this study we use a novel approach to quantitatively investigate mechanical and interfacial properties of clonal β-cells using AFM-Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated for ...
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Extraction of accurate biomolecular parameters from single-molecule force spectroscopy experiments
... ingle-molecule force spectroscopy (SMFS) techniques are increasingly being ap- plied in biology to understand biomole- cular interactions and protein dissociation under the application of ...applied ...
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Efficient unfolding pattern recognition in single molecule force spectroscopy data
... single-molecule force spectroscopy (SMFS) method was established for experimental investigations on pro- teins (membrane and globular) and cells ...characteristic Force- Distance (F-D) curve, as ...
11
Verdorfer, Tobias (2018): Multiplexed single-molecule force spectroscopy and activity studies on cellulosomes. Dissertation, LMU München: Fakultät für Physik
... unfolding force distributions of the ddFLN4 fingerprint domain (left column, blue) and of the cohesins under investigation (mid- dle column, red), as well as the unbinding force distribution of the ...
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Application of Fuzzy Logic Algorithm to Single Molecule Force Spectroscopy of the Streptavidin Biotin System
... Atomic Force Microscopy and the Measurement Conditions We employed a commercial AFM system (MFP-3D Bio, Oxford Instruments, USA) equipped with a liquid ...triggering force and loading rate were fixed at 150 ...
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Use the Force: Evaluating Force-sensitive Authentication for Mobile Devices
... for force- PINs. Furthermore, force patterns like in force-PINs can also be added to character/digit passwords with variable length and Android unlock patterns to make them resilient to shoulder ...
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