Functional expression in yeast for kinetic comparison
Beta glucosidase from thermotolerant yeast Pichia etchellsii: Gene sequencing, cloning and functional expression in Methylotrophic yeast Pichia pastoris
... The yeast is easy to genetically manipulate, provides soluble, correctly folded recombinant proteins and has potential to grow to a very high cell density (Kim et ...pastoris expression system has also been ...
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Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols
... 1 Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols Björn D.M. Bean 1,† , Colleen J. Mulvihill 2,† , Riddhiman K. Garge 2 , Daniel R. Boutz ...
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Functional expression and characterization of the C. elegans G-protein-coupled FLP-2 Receptor (T19F4.1) in mammalian cells and yeast
... Based on pharmacological evidence with a phospholipase inhib- itor, we conclude that FLP-2R signals through the G q a pathway in CHO cells. This association was confirmed in the yeast system; FLP2-induced growth of ...
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A yeast expression system for functional and pharmacological studies of the malaria parasite Ca2+/H+ antiporter
... the yeast cells showed that whilst PfCHA seems to locate to the mitochondrion of ...in yeast PfCHA is sorted to the ...further functional and pharmacological studies. Discussion: The ...
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Study of Expression of Sugarcane Sucrose Transporter CDNA in Yeast
... into yeast (Saccharomyces cerevi- siae) and grown in minimal medium (SD–urasil) as selection ...The functional expression was studied by growing yeast in YPD medium with 2% Sucrose, then the ...
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Functional Partitioning of Yeast Co Expression Networks after Genome Duplication
... Department of Genetics, Trinity College, University of Dublin, Dublin, Ireland Several species of yeast, including the baker’s yeast Saccharomyces cerevisiae, underwent a genome duplication roughly 100 ...
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Testing the kinetic energy functional: Kinetic energy density as a density functional
... noninteracting kinetic energy of density functional theory is ...density functional determined completely by the kinetic energy functional ...direct comparison between exact and ...
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Expression of Functional Influenza Virus RNA Polymerase in the Methylotrophic Yeast Pichia pastoris
... the functional viral RNA polymerase sufficient for analysis of its structure-function relationships, the cDNAs for RNA segments 1, 2, and 3 of influenza virus A/PR/8, each under independent control of the alcohol ...
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Functional Heterologous Protein Expression by Genetically Engineered Probiotic Yeast Saccharomyces boulardii
... To assess the potential for S. boulardii auxotrophic mutants to serve as drug delivery vehicles, we tested their ability to maintain production of recombinant protein in vivo. For these oral gavage experiments, we ...
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A Functional Module of Yeast Mediator That Governs the Dynamic Range of Heat-Shock Gene Expression
... We thank Xin Liu, Elena V. Sambuk, Geraldine Alba, Jorge Herrera- Diaz, Christina Venturi, Beth Zweig, Staci Deaton, Jana K. Shirey, and Lindsey Garner for technical assistance; Mike Hampsey for helpful suggestions ...
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Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities
... gene expression is of par- ticular importance when developing yeast cell factories ...good expression levels of the appropriate genes throughout the bioprocess—including during and after the diauxic ...
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yStreX: yeast stress expression database
... genome-wide expression analyses have been often used to study how expression of genes changes in response to various environmental ...model, yeast Saccharomyces ...of yeast physiology, we have ...
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Functional and topological characterization of transcriptional cooperativity in yeast
... Conclusions In this work, we studied the role of transcriptional cooperativity in the control of regulatory programs in yeast. Our results suggest that transcriptional coopera- tivity has a specific role focused ...
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Functional metabolomics describes the yeast biosynthetic regulome
... latter were highly enriched for metabolic alterations, but not gene-specific transcription factors, which created much smaller effects. Potentially, an evolutionary explanation for this finding exists. Metabolism is one ...
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Functional and regulatory profiling of energy metabolism in fission yeast
... fission yeast to identify over 200 genes required for respiratory ...budding yeast. We applied gene-expression profiling approaches to compare steady-state fermentative and respiratory growth and to ...
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Functional and Regulatory Profiling of Energy Metabolism in Fission Yeast
... in yeast cells can be easily manipulated by supplying different carbon sources: on glucose yeast rapidly proliferates by fermentation, analogous to tumour cells growing by aerobic glycolysis, whereas on ...
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Functional inclusion bodies produced in the yeast Pichia pastoris
... the yeast Pichia pastoris (Komagataella ...recombinant yeast [25–29], there are no studies reporting the controlled production of biologically active IBs or IB-like materials so far, and only a limited ...
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Comparative gene expression between two yeast species
... these expression datasets more carefully, we developed a statistical metric, Local Network Simila- rity (LNS), to assess correlation patterns of ...bayanus expression compen- dium with a collection of ...
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Gene Expression From Random Libraries of Yeast Promoters
... In vitro-generated promoter sequences (constructed as described in materials and methods and below) were cotransformed into dit1/dit1 cells together with linearized reporter vector (Figure 1C), and cells con- taining ...
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Systematic Management and Analysis of Yeast Gene Expression Data
... large-scale expression studies on the basis of oligonucleotide arrays, DNA microarrays, Serial Analy- sis of Gene Expression (SAGE), and other techniques (Velculescu et ...large expression data sets ...
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