Functional metagenomics
Functional Metagenomics Reveals an Overlooked Diversity and Novel Features of Soil Derived Bacterial Phosphatases and Phytases
... ABSTRACT Phosphatases, including phytases, play a major role in cell metabolism, phosphorus cycle, biotechnology, and pathogenic processes. Nevertheless, their dis- covery by functional metagenomics is ...
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Meeting Report: 1st International Functional Metagenomics Workshop May 7–8, 2012, St. Jacobs, Ontario, Canada
... tional Metagenomics Workshop (IFMW) was orga- nized and hosted by researchers from the Universi- ty of Waterloo and funded by the University of Wa- terloo, Ontario Genomics Institute (OGI), and Natu- ral Sciences ...
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Structure Elucidation and Biochemical Characterization of Environmentally Relevant Novel Extradiol Dioxygenases Discovered by a Functional Metagenomics Approach
... a functional metagenomics ap- proach to overcome the limitation of cultivability of microbes under standard laboratory conditions in order to isolate novel dioxygenases capable of degrading recalcitrant ...
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Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing
... the functional expression library (Table ...considerable functional overlap between, and variety within, GH families, this is likely to be an overestimate of the abundance of these specific enzyme ...
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Functional Metagenomics Reveals a New Catalytic Domain, the Metallo-β-Lactamase Superfamily Domain, Associated with Phytase Activity
... Phytase activity has been reported to be restricted to only four classes of protein phosphatases with different catalytic mechanisms. The histidine acid phosphatases (HAP-phy) represent the most extensively studied class ...
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Mining for hemicellulases in the fungus-growing termite Pseudacanthotermes militaris using functional metagenomics
... encodes several GHs and proteins that are homologous to araA, araB, araD et araE (genes belonging to the arabinan utilization operon) found in many bacteria in- cluding Bacillus subtilis and Geobacillus stearother- ...
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Open resource metagenomics: a model for sharing metagenomic libraries
... facilitating functional screening in virtually any surrogate microbial host for which there are available plasmid ...resource metagenomics to extend the utility of functional metagenomics ...
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Reduced Susceptibility to Antiseptics Is Conferred by Heterologous Housekeeping Genes
... Previously, novel antibiotic resistance genes have been isolated from the oral metagenomic libraries by using functional metagenomics, including the tetracycline resistance gene tet(37) [r] ...
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Metagenomics: aid to combat antimicrobial resistance in diarrhea
... Metagenomic screening of gut resistome has helped in the identification of antibiotic resistance profile in human infants and adults and provided a sound under- standing of the resistome development of the gut. In ...
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Developing a Bacteroides System for Function-Based Screening of DNA from the Human Gut Microbiome
... ABSTRACT Functional metagenomics is a powerful method that allows the isolation of genes whose role may not have been predicted from DNA ...Typically, functional screens are carried out using ...
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Soil metagenomics: a prospective approach for novel enzyme discovery
... cell. Functional analysis has identified novel antibiotics (Courtois et ...for functional screening of metagenomic libraries is to use host strains or mutants of host strains that require heterologous ...
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Genome-resolved metagenomics of sugarcane vinasse bacteria
... bacteria unknown at the phylum level. Furthermore, most of the bins recovered here were partly uncharac- terized at the species level, supporting the idea that we obtained genomes from novel strains of bioethanol con- ...
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Evaluating the Information Content of Shallow Shotgun Metagenomics
... and functional resolution, it is often prohibitively expensive for large-scale ...the functional profile; thus, there is currently no widely accepted approach to affordable, high-resolution, taxonomic, and ...
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VSEARCH : a versatile open source tool for metagenomics
... targeted metagenomics studies produce many millions of sequences, and require open-source, fast and memory efficient tools to facilitate their ecological ...
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Microbiome and Metagenomics: Statistical Methods, Computation and Applications
... Figure 2.9: Analysis of longitudinal results using species-level data. A) Analysis based on distance to the centroid of healthy controls. B) Regression analysis of the effects of antibio[r] ...
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Metagenomics in ophthalmology: current findings and future prospectives
... MDS may supplement or replace numerous and expen- sive diagnostic assays and procedures currently employed and improve patient outcomes. This approach will allow a far more comprehensive characterisation of the aeti- ...
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Dirichlet multinomial mixtures : generative models for microbial metagenomics
... Dirichlet prior [25], but the extension of that prior to a mixture of Dirichlet components in this context is completely novel, as is the explicit association of each Dirichlet component with a different metacommunity. ...
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The Composition and Downward Vertical Transport of Particulate Phosphorus in the Cariaco Basin, Venezuela
... A viral metagenomics study by Willner et al. (2009) on sputum samples from five CF and five non-CF (including one asthmatic and one CF spouse) individuals found distinct differences between the respiratory tract ...
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DNA Based Methods in Intelligence - Moving Towards Metagenomics
... In intelligence, a precise taxonomic assignment may not even be needed to provide useful information. More general microbial sequence profiles can be used for the geo-localisation of a sample or in predictive modelling. ...
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Diagnostic metagenomics : potential applications to bacterial, viral and parasitic infections
... Before discussing diagnostic metagenomics, it is worth brie fl y reviewing the problems with existing approaches. Even though we are now well into the 21st century, diagnostic bacteriology is still largely reliant ...
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