Fusion constructs
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors
... the fusion constructs appeared to be par- tially cleaved at the level of the linker during the purifica- ...DsbC-EYFP fusion (data not ...as fusion partners ...
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Zinc-Regulated Genes in Saccharomyces cerevisiae Revealed by Transposon Tagging
... Expression of these ZRG - lacZ fusion constructs was 6 and 11) was not visibly affected by disruption of ZAP1 , and ZRE-like motifs were correspondingly absent from strongly zinc regulat[r] ...
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The Formamidase Gene of Aspergillus nidulans: Regulation by Nitrogen Metabolite Repression and Transcriptional Interference by an Overlapping Upstream Gene
... Effects of carbon starvation on fmdS expression: The absence of a carbon source led to loss of response to nitrogen starvation of fmdS::lacZ expression (Figure 2). Earlier studies on formamidase levels showed a reduced ...
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C/EBPα-p30 protein induces expression of the oncogenic long non-coding RNA UCA1 in acute myeloid leukemia
... promoter-luciferase fusion constructs containing the wild-type UCA1 promoter or a mutant derivative lacking the putative C/EBPα binding site and tested the resulting luciferase activity in K562 cells ...
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A new fusion protein platform for quantitatively measuring activity of multiple proteases
... optimal fusion con- struct, we designed a library of DAL-fusion constructs, by combining seven different protease cleavage sequences (R3P, TEVp, factor Xa, Ssp DnaB intein, Sce VMA1 intein, thrombin ...
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Probing the Structural States of Human Immunodeficiency Virus Type 1 Pr55gag by Using Monoclonal Antibodies
... protein) fusion constructs are commonly used to study human immunodeficiency virus type 1 assembly, yielding diffuse signals throughout the cytoplasm along with punctate signals routinely described as ...
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A Pmk1-Interacting Gene Is Involved in Appressorium Differentiation and Plant Infection in Magnaporthe oryzae
... PIC5-GFP fusion constructs and transformed them into the pic1 and pic5 knockout mutants GA22 and ML4, ...PIC5-GFP fusion constructs, 118-kDa and 56-kDa bands of the predicted Pic1-GFP and ...
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The I Domain Is Required for Efficient Plasma Membrane Binding of Human Immunodeficiency Virus Type 1 Pr55Gag
... Gag-GFP fusion protein to include CA and SP1 did not significantly alter the observed intracellular distribution of the ...GFP fusion constructs revealed that the region responsible for the ...
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Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras
... of fusion part- ners in different orders into a growing vector ...of fusion enzymes on one polypeptide has been shown to be impor- tant for efficient catalytic activity and was even shown to be advantageous ...
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A 38-Amino-Acid Sequence Encompassing the Arm Domain of the Cucumber Necrosis Virus Coat Protein Functions as a Chloroplast Transit Peptide in Infected Plants
... vector constructs used for expressing the GFP fusion ...these constructs [with the exception of GFP/arm( ⫹ SVRI)/CNVexp] was His tagged at the carboxyl terminus ...of fusion proteins in cells ...
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Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems
... different fusion constructs have been de- signed, produced and separately introduced into Pichia GS115( his 4 ) cells, using as a starting point the yeast codon optimised saporin (SAPopt) sequence which has ...
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Receptor for activated C kinase-1 facilitates protein kinase C-dependent phosphorylation and functional modulation of GABA(A) receptors with the activation of G-protein-coupled receptors
... receptor 1 subunit constructs. This allowed us to deduce that the PKC was binding to the region between regions 405 and 415 (IRRRASQLKVKI) within the intracellular domain of the 1 subunit, straddling the ...
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Reduced Susceptibility to VIRIP-Based HIV-1 Entry Inhibitors Has a High Genetic Barrier and Severe Fitness Costs
... Previous studies showed that VIRIP-based inhibitors do not show cross-resistance to other antiretroviral agents (9) and were safe and effective in a clinical trial (11). Here, we show that the genetic barrier to ...
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Comparison of Surgical Outcomes in Thoracolumbar Fractures Operated with Posterior Constructs Having Varying Fixation Length with Selective Anterior Fusion
... The present retrospective study was carried out in 94 patients with single vertebral level traumatic burst fractures between T11 and L2 from February 2001 to February 2006. All the patients were subjected to posterior ...
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Peptide-Mediated Liposome Fusion: The Effect of Anchor Positioning
... the constructs are still able to form coiled-coils effectively when the complementary peptide is added, and the Figure ...peptide constructs incorporated into ...
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The development of the ‘self directed learning readiness scale in biology’ (SDLRSbio) for pre university biology students
... After the first Delphi round, all 6 pre-determined constructs were accepted by the experts. However, some of the constructs were amended to focus in the study of biology. For example “Learning Skills ...
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Fluorescence Resonance Energy Transfer-Based Intracellular Assay for the Conformation of Hepatitis C Virus Drug Target NS5A
... FRET substrates for high-throughput screening (30, 38) and FlAsH dye detection of HCV core in cells assembling particles (7) have been used in several innovative studies of HCV recently, but to our knowledge they have ...
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EFFECT OF INTEGRATION OF GREEN CONSTRUCTS AND TRADITIONAL CONSTRUCTS OF BRAND ON GREEN PURCHASE INTENTION OF CUSTOMERS
... new constructs, such as green image, green trust, green satisfaction, and the equity of a green brand, all of which have attempted to create a positive attitude towards the green brand (Chen, 2010; Chen & ...
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Constructs and theoretical concepts: introduction
... these constructs might be seen as time specific and that, were this book being written in a dif- ferent decade, the prioritisation of contributions might be different, nevertheless they all present ...
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The Evolution of Social Constructs
... The first thing I shall do is provide an elementary introduction to evolution through Darwin’s natural selection, followed by an introduction to evolutionary social science (soci[r] ...
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