Ganglioside GM1
Interaction between Simian Virus 40 Major Capsid Protein VP1 and Cell Surface Ganglioside GM1 Triggers Vacuole Formation
... the ganglioside GM1, which serves as the cellular receptor for the ...surface GM1 plays a key role in SV40 infection-induced ...for GM1 binding fail to induce vacuolization, even though they ...
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A Single Native Ganglioside GM1 Binding Site Is Sufficient for Cholera Toxin To Bind to Cells and Complete the Intoxication Pathway
... Ganglioside GM 1 ELISA. Ninety-six-well microtiter plates were coated overnight with 50 l of 2-fold serial dilutions of ganglioside GM 1 (Supelco, Sigma-Aldrich, St. Louis, MO) in PBS, starting at 150 nM, ...
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Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing Escherichia coli
... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ...
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Monoclonal antibodies against Escherichia coli heat stable toxin (STa) and their use in a diagnostic ST ganglioside GM1 enzyme linked immunosorbent assay
... The straih previously reported to produce STaT that was negative ih the GM1-ELISA was also negative in the infant mouse test even when concentrated culture filtrate was tested.. enteroco[r] ...
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Development of an Immunoassay for Rapid Detection of Ganglioside GM1 Mimicry in Campylobacter jejuniStrains
... to ganglioside-mimicking pure LPSs was compared to ligand interactions with miniphenol-water-extracted LPSs from the same nine ...the ganglioside detection re- agents had the same specificities for ...
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A Single Native Ganglioside GM1-Binding Site Is Sufficient for Cholera Toxin to Bind to Cells and Complete the Intoxication Pathway
... Ganglioside GM 1 ELISA. Ninety-six-well microtiter plates were coated overnight with 50 l of 2-fold serial dilutions of ganglioside GM 1 (Supelco, Sigma-Aldrich, St. Louis, MO) in PBS, starting at 150 nM, ...
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Evidence for Budding of Human Immunodeficiency Virus Type 1 Selectively from Glycolipid-Enriched Membrane Lipid Rafts
... the ganglioside GM1, which is known to partition preferentially into lipid ...CD59, GM1, and a lipid raft-specific fluo- rescent lipid, DiIC 16 (3), in uropods of infected Jurkat ...
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The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100
... the ganglioside GM1, due to its specific binding by the cholera toxin, is frequently used as a marker in studies on membrane ...tritium-labeled GM1 ganglioside taken up by HL-60 cells from ...
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Uptake Pathway of Polyomavirus via Ganglioside GD1a
... of ganglioside GM1 to C6 cells renders them susceptible to SV40 (38), presumably enabling a pathway sim- ilar to that of Py in GD1a-supplemented C6 cells and compa- rable to the pathway in monkey cells that ...
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Perturbing Gangliosides In-vitro Using Ambroxol as a Treatment for Neurodegeneration
... complex ganglioside GM1 is degraded to simpler species GM2 and ...a ganglioside modifying agent that has been shown to perturb enzymes in the ganglioside degradation pathway, in order to ...
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Lipid Membrane Based Biosensors
... [30]. Ganglioside GM1 (a natural cholera toxin receptor) was incorporated on stabilized lipid films on graphene electrodes, providing adequate selectivity for the detection of cholera toxin over a wide ...
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Macropinocytosis Is the Entry Mechanism of Amphotropic Murine Leukemia Virus
... A-MLV localizes to vacuolar structures enriched in conca- navalin A binding sites. Finally, we sought to obtain a light-mi- croscopic correlate of our observations. HeLa cells expressing Rac1-V12G (to increase the number ...
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Saccharomyces cerevisiae Npc2p Is a Functionally Conserved Homologue of the Human Niemann-Pick Disease Type C 2 Protein, hNPC2
... cholesterol and the ganglioside GM1 in late endosomes and lysosomes (34). We tested whether yeast Npc2p could reverse the hNPC2 ⫺ / ⫺ lipid accumulation defects. 6), as well as wild-type[r] ...
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N-Glycolyl GM1 Ganglioside as a Receptor for Simian Virus 40
... to ganglioside GM1(Gc) (Fig. 5, position 185). Ganglioside GM1(Ac), position 199, which was shown previously to bind SV40, was a considerably weaker ...
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Mutations in the GM1 Binding Site of Simian Virus 40 VP1 Alter Receptor Usage and Cell Tropism
... the ganglioside GM1 as a receptor that mediates cell bind- ing and ...VP1 GM1 binding site. Two of these mutants were completely resistant to GM1 neutralization, were no longer stimulated by ...
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INTRAUTERINE DETECTION OF GM1 GANGLIOSIDOSIS, TYPE 2
... Examination of the fetal tissues revealed marked deficiency of p-galactosidase activity, increased cerebral GMI ganglioside and abnormal hepatic lysosomes confirming the[r] ...
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Gayk, Laura (2019): Entwicklung und Anwendung auf antiidiotypischen Antikörpern basierender Verfahren zum Nachweis von Aflatoxinen und T-2 Toxin. Dissertation, LMU München: Tierärztliche Fakultät
... Aufgrund der bestehenden Selbsttoleranz gegenüber dem konstanten Teil von Immunglobulinen ist im syngenen Ansatz bei Immunisierung mit einen full-length mAk nur ein kleiner Anteil (hoch variable Region) immunogen. Eine ...
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Rotaviruses specifically bind to the neutral glycosphingolipid asialo-GM1.
... Furthermore, purified monoclonal antibodies against GAl were able to partially inhibit rotavirus replication in plaque assays but were unable to inhibit a much larger virus inoculum in a[r] ...
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Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
... 1 GM1 molecule by CTB brings GM1s into close contact, facilitating the coales- cence of small membrane protrusions into a single larger membrane protrusion (Figure ...
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Influenza virus-induced encephalopathy in mice: interferon production and natural killer cell activity during acute infection.
... NK cells bear the differentiation antigen asialo-GM1 2, 11, 18, 34, and treatment of mice with anti-asialo-GM1 in vivo has been shown to significantly reduced NK cell activity without af[r] ...
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