gene amplification
MET gene amplification in gliomas to determine their incidence
... MET amplification can be a major driver after acquired resistance to epider- mal growth factor receptor (EGFR) inhibitors, because of the cross-talk with other RTK family members ...MET gene ...
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MET overexpression and gene amplification in NSCLC: a clinical perspective
... The catalytic activity of MET can be blocked by small molecule inhibitors that generally compete for the adenos- ine triphosphate (ATP) binding site in the tyrosine kinase domain of MET, thus preventing the downstream ...
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Telomerase reverse transcriptase gene amplification in hematological malignancies
... chromosome ends for stabilization of telomere length in attaining cellular immortality and may therefore be a critical step in carcinogenesis. Expression of significant levels of telomerase can dramatically increase ...
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Investigation of gene amplification and expression in prostate cancer
... manufacturer’s instructions. Ing of male reference DNA and Cosh DNA also under 2 rounds o f DOP-PCR amplification. Briefly in a total volume o f 50|il using 5(0.1 of test DNA template or 5|0.1 of reference ...
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Rapid Divergence of Prolamin Gene Promoters of Maize After Gene Amplification and Dispersal
... ABSTRACT Seeds have evolved to accommodate complicated processes like senescence, dormancy, and germination. Central to these is the storage of carbohydrates and proteins derived from sugars and amino acids synthesized ...
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Identification of Toxoplasma gondii infections by BI gene amplification
... gondii by PCR in clinical specimens and tissue samples that were obtained postmortem from a bone marrow recipient with cerebral toxoplasmosis and from three congenitally infected childre[r] ...
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Genome position and gene amplification
... for amplification are thought to be present in amplicons in human cancers, including 8p11-p12 and 17q12 in breast cancer [22-24], ...because gene expression is correlated with copy ...
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MET amplification as a potential therapeutic target in gastric cancer
... target gene from the ratio of the copy number of the gene to that of the ...of gene amplification in a large number of tumor specimens would thus benefit from the availability of a ...
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Analysis in Carcinoma Stomach Her-2/neu Positivity by Immuno-histochemistry
... 40 Tanner M, Hollmen M, Junttila TT, et al: Amplification of HER-2 in gastric carcinoma: association with topoisomerase II alpha gene amplification, intestinal type, poor prognosis and s[r] ...
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Overexpression of a cytochrome P450 monooxygenase, CYP6ER1, is associated with resistance to imidacloprid in the brown planthopper, Nilaparvata lugens
... P450 gene belonging to the CYP6 family (CYP6CM1, CYP6CY3 and CYP6G1 respectively) contributes to resis- tance to imidacloprid (Daborn et ...via gene amplification (Li et ...CYP6ER1 gene copy ...
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Detection of Chromosomal Abnormalities with Different In Situ Hybridisation Techniques – the Usefulness in the Qualification of Cancer Patients for Molecularly-Targeted Therapies
... Proper qualification of patients with cancer for an effective treatment regiment is essential to rationalize therapy benefit and costs. The early detection of genetic disorders that are responsible for the stimulation of ...
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Strong nuclear EGFR expression in colorectal carcinomas is associated with cyclin-D1 but not with gene EGFR amplification
... with gene amplification, found in approximately 6% of CRC sam- ples, while moderate mEGFR was observed in those tumors that showed chromosome 7 ...EGFR amplification has been reported to correlate ...
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Amplification of Aminoglycoside Resistance Gene aphA1 in Acinetobacter baumannii Results in Tobramycin Therapy Failure
... Confirmation of gene amplification and expression by qPCR. When normalized to the single-copy genes secE and rpiN (21) by quantitative PCR (qPCR), isolate MRSN 57 had an average of 10 ( ⫾ 2) copies ofaphA1, ...
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Expression of Early and Late Simian Virus 40 Transcripts in the Absence of Protein Synthesis
... This amplification of early viral mRNA steady-state levels is probably not due to inhibition of synthesis of the early A gene product since the same degree of drug-specific amplification[r] ...
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Isolation, amplification and sequence analysis of the gene encoding Enolase from lymphatic filarial parasite Brugia malayi
... isolation, amplification and sequence analysis of the gene encoding enolase from lymphatic filarial parasite, ...the amplification of the complete coding sequence of enolase from infective stage ...
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Hypoparathyroidism, Sensorineural deafness and renal disease (Barakat syndrome) caused by a reduced gene dosage in GATA3: a case report and review of literature
... The HDR syndrome, also known as Barakat syndrome is as an autosomal dominant rare genetic disorder [2], primarily caused by haploinsufficiency of GATA3 gene on chromosome 10p14 [4]. GATA3 is expressed in the ...
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A centrosome clustering protein, KIFC1, predicts aggressive disease course in serous ovarian adenocarcinomas
... the gene ontologies of signifi- cantly overexpressed genes associated with the KIFC1-high group utilizing the PANTHER classification ...the Gene set enrichment analysis ...cycle gene sets ...enriched ...
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Equine Abortion Associated with the Borrelia parkeri B turicatae Tick Borne Relapsing Fever Spirochete Group
... competent Escherichia coli (TOPO TA cloning kit for sequencing; Invitrogen, Carlsbad, Calif.). Both strands of the cloned fragment were sequenced by automated fluorescent cycle sequencing (per- formed by Davis ...
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A spontaneous chromosomal amplification of the ADH2 gene in Saccharomyces cerevisiae.
... The amplification of ADH2 was most likely a two-step process in which ADH2 was first inserted within the rDNA followed by an increase in the number of copies of ADH2[r] ...
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Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) through the implementation of a High Resolution Melting (HRM) genotyping assay
... The use of the HRM methodology allowed the adequate and congruent discrimination of the T. cruzi genotypes for the strains and reference clones used. Many problems, such as the need for sequencing, the degree of ...
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