Gene network between osteoblasts and osteoclasts
Lack of effect of adenosine on the function of rodent osteoblasts and osteoclasts in vitro
... rodent osteoblasts or mouse osteoclasts. In rat bone marrow osteoblasts, 2-chloroadenosine exerted small effects on TNAP activity and bone formation, but only when added to cell cultures in extremely ...
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Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co Cultured with Osteoblasts
... cellic gene expression of RANK and CA II in vitro and vivo have been reported [14] ...the gene expression of RANK and NFATc1 in rat osteoclasts co-cultured with osteoblasts and researched the ...
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Effects of salubrinal on development of osteoclasts and osteoblasts from bone marrow derived cells
... macrophages and CFU-macrophages, as well as formation of mature osteoclasts in a dosage-dependent manner. Salubrinal also suppressed migration and adhesion of pre-osteoclasts and increased the number of ...
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Internal control genes for quantitative RT PCR expression analysis in mouse osteoblasts, osteoclasts and macrophages
... Alexandre S Stephens * , Sebastien R Stephens and Nigel A Morrison Abstract Background: Real-time quantitative RT-PCR (qPCR) is a powerful technique capable of accurately quantitating mRNA expression levels over a large ...
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Effect of Beta-Blockers on Number of Osteoblasts and Osteoclasts in Alveolar Socket Following Tooth Extraction in Wistar Rats
... demonstrated that a non-specific β2-adrenergic recep- tor antagonist like propranolol can enhance bone for- mation in female rats that had undergone ovariectomy. If beta-blockers are truly capable of preventing bone ...
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A single administration of combination therapy inhibits breast tumour progression in bone and modifies both osteoblasts and osteoclasts
... stained osteoclasts per millimetre trabecular bone surface was scored using a Leica RMRB upright microscope with a 10x objective and OsteoMeasure software ...sections, osteoblasts were scored per millimetre ...
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Evidence based anti osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells
... of osteoblasts to mineralize the collagen ...that osteoblasts treated with KFX possessed significantly higher rate of mineralization compared with other tested ...nodules. Osteoclasts are ...
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The puzzle of lactational bone physiology: osteocytes masquerade as osteoclasts and osteoblasts
... A second mechanism of skeletal mineral loss during lactation is osteocytic osteolysis — a process by which osteocytes function like osteoclasts to resorb mineral from their sur- roundings (10, 11). The ability of ...
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Potential anti osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro
... Epimedii [8] and Curcuma longa [9-12]). In order to identify more candidates of herbal extracts that have therapeutic effects not only in OP, but are also effective in the treatment of oral and skeletal diseases, an ...
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Recruitment of new osteoblasts and osteoclasts is the earliest critical event in the pathogenesis of human multiple myeloma
... A significant enhancement of osteoblastic recruitment with an increased generation of new osteoclasts is an early critical event in the pathogenesis of human MM. Of particular impor- tan[r] ...
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Original Article Different functions of the two isoforms of osteopontin (iOPN and sOPN) in the proliferation and differentiation of osteoblast and osteoclast: iOPN inhibits osteoclasts’ proliferation while sOPN stimulates osteoblasts’ differentiation and osteoclasts’ resorption
... TRAP staining To confirm the generation of multinucleated osteoclast-like cells, RAW 264.7 cells were seeded in 48 well plates at a density of 6×10 4 cells/well with an osteogenic induction me- dium as well as the ...
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Effects of cobalt and chromium ions at clinically equivalent concentrations after metal-on-metal hip replacement on human osteoblasts and osteoclasts: implications for skeletal health
... concentration range previously reported in serum and within hip synovial fluid in patients after MOMHR. SaOS-2 cells were cultured with Co 2+ , Cr 3+ and Cr 6+ for 3 days after which an MTS assay was used to assess cell ...
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Osteoclasts and Microgravity
... by osteoblasts and osteocytes. Mechanically stimulated osteoblasts inhibit osteoclastogenesis by producing osteoprotegerin; this decoy receptor binds RANKL and prevents it from binding to receptors on ...
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Relationship between abnormal osteoblasts and cellular immunity in multiple myeloma
... of MM which due to the increased number and activa- tivity of osteoclasts. In recent years, the molecular bio- logy studies of MBD advanced a lot. The function and pathological changes of osteoblasts ...
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Abstract: Bone homeostasis depends on the interplay between bone resporption by osteoclasts and
... interplay between bone resporption by osteoclasts and bone formation by ...communication between osteoclasts and osteoblasts are critical to bone cell ...
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Osteoclasts are multinucleated cells that resorb
... niche. Osteoclasts differentiate from monocyte/macrophage lineage cells through two sequential cell cycle – related ...by osteoblasts. QuOPs dif- ferentiate into osteoclasts without cell cycle ...
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Original Article Correlation between autophagy of osteoblasts and oxidative stress of osteoporosis rats
... Various factors including drugs, hormones, nutrients and aging can cause imbalance of bone absorption/formation. Studies showed that the over-activation of osteoclasts and abnormal function of osteoblasts ...
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Osteoclasts: Multifaceted Molecule in Vesicular Trafficking
... to the functional secretary domain by transcytosis and their secretion into the circulation. Migration and Targeting Type I collage peptides, alpha-2-HS glycoprotein and osteocalcin are known to elicit a dose-dependent ...
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Cells of the synovium in rheumatoid arthritis Osteoclasts
... milieu between the metabolically active part of the plasma membrane of the osteoclast, the ruffled border, and the bone ...allow osteoclasts to invade bone and create a resorption pit, which can latter be ...
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Gene expression analysis in human osteoblasts exposed to dexamethasone identifies altered developmental pathways as putative drivers of osteoporosis
... by osteoblasts as well as bone resorption by osteoclasts are the key biological events in ...osteoblast gene expression was determined using oligonucloeotide ...key gene clusters altered in ...
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