Genetically encoded calcium indicators (GECIs)
Genetically encoded calcium indicators for multi color neural activity imaging and combination with optogenetics
... Genetically encoded calcium indicators (GECIs) are powerful tools for systems ...2-color calcium imaging both within the same cell (registering mitochondrial and somatic [Ca 2 + ]) and ...
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Mank, Marco (2008): Improved Genetically-Encoded Calcium Indicators Based on Troponin C. Dissertation, LMU München: Fakultät für Biologie
... genetically-encoded calcium indicator is available so ...of genetically-encoded calcium indicators that are based on calmodulin ...
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Heim, Nicola (2005): Genetically Encoded Calcium Indicators Based on Troponin C and Fluorescent Proteins. Dissertation, LMU München: Fakultät für Biologie
... BSTRACT Genetically encoded calcium probes allow the visualization and quantification of intracellular calcium dynamics with great specificity and ...all genetically encoded ...
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Imaging the awake visual cortex with a genetically encoded voltage indicator.
... between the two sources, as follows: the voltage signal is primarily neural, and the hemodynamic signal is primarily vascular. Though we did not explore longitudinal imaging in any for- mal way, we generally found that ...
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Direnberger, Stephan (2012): Investigating expression, function and biocompatibility of the genetically encoded calcium indicator TN-XXL in transgenic mice. Dissertation, LMU München: Fakultät für Biologie
... cellular calcium levels by fluorescent probes has become an important method to visualize cellular signaling ...of genetically encoded calcium indicators (GECIs) strongly contributed to ...
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A Versatile Method for Viral Transfection of Calcium Indicators in the Neonatal Mouse Brain
... fluorescent genetically encoded calcium indicators (GECIs), especially GCaMP6 ( Chen et ...two-photon calcium imaging with GECIs typically consists of injecting a recombinant ...
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Geiger, Anselm (2017): Biophysical analysis of the FRET-based genetically encoded calcium indicator TN-XXL. Dissertation, LMU München: Fakultät für Biologie
... fluorescence-based indicators depends on two main factors: firstly the reaction of the indicator to a certain change in the analyte concentration, specified as its affinity, and secondly a detectable change of at ...
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Ihring, Alexandra (2006): Characterization and Modification of Genetically Encoded Indicators to Monitor Neural Activity in Drosophila melanogaster. Dissertation, LMU München: Fakultät für Biologie
... 4.10 Indicators Tagged to the Active Zone In all of the previous experiments indicators were expressed in the ...system genetically encoded calcium indicators can be directed to ...
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Using a genetically encoded sensor to identify inhibitors of Toxoplasma gondii Ca2+ Signalling
... Genetically encoded calcium indicators provide excellent reproducibility and circumvent many problems associated with loading and compartmentalization of chemical ...such indicators can ...
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Elucidating astrocyte and neuron interactions using genetically encoded fluorescent indicators
... HEK293 cells were plated in 10 cm cell culture dishes at a density of 4 x 10 6 cells per dish and allowed to reach 50-60% confluence overnight. A total of 20 ng of plasmid mix was calcium- phosphate transfected ...
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Litzlbauer, Julia (2015): Engineering and screening of genetically encoded FRET Calcium indicators. Dissertation, LMU München: Fakultät für Biologie
... periplasm), we used ionomycin to transport Ca 2+ across the inner membrane. As ionomycin did not seem to be able to cross the outer membrane however, we utilized polylysine in order to further increase outer membrane ...
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Genetically encoded Ca
... both indicators. In general, most selected lines expressed the indicators weakly and were poorly responsive, or expressed the transgene concentrated in a punctate pattern; these findings may suggest that ...
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Genetically encoded biosensors for lignocellulose valorization
... of genetically encoded biosensors as tools to achieve these goals, and describes future avenues for techno- logical advancement in the ...review genetically encoded biosensors that have been ...
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A genetically encoded biosensor for visualizing hypoxia responses in vivo
... Whereas embryonic epidermal and tracheal cells show varying levels of reporter accumulation, apparently reflecting the individual state of each cell at the moment of the ana[r] ...
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Genetically encoded division machinery for cell free synthetic biology
... The de novo construction of cellular life requires, in part, the assembly of components that confer the ability to replicate. Herein we describe efforts to reconstitute parts of the Escherichia coli cell division ...
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Zebrafish embryonic explants undergo genetically encoded self-assembly
... Abstract Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this ...
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Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging
... nanoparticles, genetically encoded probes offer easier labeling of defined cells within tissues or proteins of interest within a cell, have higher stability in vivo, and eliminate the need for delivery of ...
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Vectors for Genetically-Encoded Tags for Electron Microscopy Contrast in Drosophila
... of genetically-encoded tags for electron microscopy such as Mini-SOG [1] and APEX [2] provides a way to simplify detection and localization of proteins using electron microscopy ...
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High-speed imaging of glutamate release with genetically encoded sensors
... (f) iGluSnFR response amplitude (green markers) of a single bouton stimulated with 1056. single APs every 10 s[r] ...
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Development of a new genetically encoded tag for correlative light electron microscopy
... 3.8 Discussion This chapter introduces FerriTag, a new genetically encoded tag for CLEM. The de- velopment and implementation of the method had been achieved for light microscopy. Initial optimization ...
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