Genome engineering
Directed genome engineering for genome optimization
... non-selectable genome editing ...plants, genome editing of non-selectable genes will only be possible by using multicellular tissue culture ...where genome editing may have happened. Once ...
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Optimizing complex phenotypes through model guided multiplex genome engineering
... multiplex genome engineering, genotyping, and predict- ive modeling ...of genome editing are used to generate a population enriched with com- binatorial diversity at the targeted ...
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Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette
... of genome engineering technology is to reduce the time and labor required to produce custom genome ...the genome, leaving no exogenous sequences outside the fl uorescent protein ...
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
... CRISPRs (clustered regularly interspaced short palin- dromic repeats) were first identified in Escherichea coli in 1987 and later shown to function as part of an adaptive immune system in bacteria and archaea (Ishino et ...
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Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9
... for genome engineering of ...a genome engineering method based on the CRISPR/Cas9 system that permits, with a single plasmid, the rapid and efficient disruption of genomic ...that ...
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Development of genome engineering technologies in cattle: from random to specific
... via genome engineering for the gain or loss of gene functions is an important ...of genome engineering, DNA micro-injection into one-cell stage embryos (zygotes) followed by embryo transfer ...
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Genome engineering for improved recombinant protein expression in Escherichia coli
... assembly genome engineering (CAGE) ...for genome engineer- ing at multiple locations with greater precision needs to be developed ...metabolic engineering is the use of a transcriptional ...
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Viral Cre-LoxP tools aid genome engineering in mammalian cells
... transformed genome editing technology, providing more efficient methods to make targeted changes in mammalian ...complex genome manipulation such as large deletion, addition, gene fusion and conditional ...
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Targeted Genome Engineering and Its Application in Trait Improvement of Crop Plants
... Targeted genome engineering refers to technologies that are used for site- specific genome modifications including gene knockout, knockin and transcrip- tional regulation [14] ...targeted ...
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CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
... The use of the clustered regularly interspaced short palin- dromic repeats (CRISPR)-Cas9 system for genome engineering (Hsu et al. 2014) has greatly facilitated the study of gene func- tion in ...
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Therapeutic Applications and Specificity of Action of Designer Nucleases for Precision Genome Engineering
... genetic engineering confers the ability to modify genomes of complex organisms, and has far-reaching applications in human medicine, agriculture, and ...the genome, in an effort to better understand the ...
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Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas
... for genome engi- neering in human cell cultures, proving that it also works in eukaryotic systems [19, ...for genome engineering due to its ease in cloning new sequence-specific nucleases and the ...
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Homing endonucleases from mobile group I introns: discovery to genome engineering
... for engineering homing endonuclease, their use as gene targeting reagents has been further facilitated by several recent ...for genome engineering ...
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Efficient homologous recombination mediated genome engineering in zebrafish using TALE nucleases
... 2003), genome editing using nucleases offers a new method for modifying endogenous ...HR-mediated genome engineering method makes it difficult for systematic generation of fluorescent protein-tagged ...
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Rewriting the blueprint of life by synthetic genomics and genome engineering
... addressed for cleavage by protein engineering. ZFNs are formed by fusing a zinc finger protein (ZFP) that comprises a tandem array of ZF motifs [48] to the FokI non-specific cleavage domain [44, 45]. Each ZF motif ...
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Making sense of GWAS: using epigenomics and genome engineering to understand the functional relevance of SNPs in non-coding regions of the human genome
... (SNPs), Genome Wide Association Studies (GWASs) have identified SNPs that are associated with many complex diseases or ...the genome for follow-up studies, (2) various computational tools that aid in ...
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Precision genome engineering in lactic acid bacteria
... the genome and easily recover the mutants without ...the genome using the Cas9 endonuclease, which is associated with a type-II clus- tered regularly interspaced short palindromic repeats (CRISPR) locus ...
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Applications of CRISPR-Cas9 mediated genome engineering
... The CRISPR-Cas9 technology is developed from type II CRISPR-Cas systems, by which bacteria degrade targeted nucleic acids [5,6]. CRISPRs are characterized features of the genomes of most bacteriophage-resistant Bacteria ...
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Targeted genome engineering in Caenorhabditis elegans
... introduce genome modifications, including random mutagen- esis by chemical reagents, ionizing radiation and transposon ...the genome through microparticle bombardment approach or by irradiation of animals ...
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Plastid Genome Engineering and its Potential Applications: A Review
... Plastid genome engineering is a credible tool for the basic biotechnological research and various innovative techniques have led to the better understanding of the complex processes involved in the plastid ...
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