Genomic Sequencing
The bisulfite genomic sequencing protocol
... The bisulfite genomic sequencing (BGS) proto- col has gained worldwide popularity as the me- thod of choice for analyzing DNA methylation. It is this popular because it is a powerful protocol and it may be ...
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Professionally Responsible Disclosure of Genomic Sequencing Results in Pediatric Practice
... between pediatricians and parents about the interpretation of the best interests standard in its psychosocial dimensions and the scope of results that should be provided to parents. It follows that parents are ethically ...
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The BabySeq project: implementing genomic sequencing in newborns
... genome sequencing into clinical medicine in ...of sequencing newborns in two cohorts, healthy and sick newborns ...plus genomic sequencing. For those in the genomic sequencing ...
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Genomic Sequencing Expansion and Incomplete Penetrance
... screening. Genomic sequencing could allow for improved diagnosis of newborns and help prevent potential consequences of carnitine depletion, such as hypoglycemia, cardiomyopathy, and hepatic ...
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Impact of Concurrent Genomic Alterations Detected by Comprehensive Genomic Sequencing on Clinical Outcomes in East-Asian Patients with EGFR-Mutated Lung Adenocarcinoma
... Comprehensive genomic sequencing. FFPE genomic DNA (150 ng) was converted into libraries and enriched for a 415 gene panel with CANCERPLEX (KEW ...cancer. Sequencing was performed on the ...
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A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
... The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however, its high rates of DNA ...
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A cost effective and universal strategy for complete prokaryotic genomic sequencing proposed by computer simulation
... second-generation sequencing technologies (Roche/454, ABI/SOLID and Illumina/ Solexa) were applied in large-scale genomic sequencing ...pyrosequencing sequencing technologies are characterized ...
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Genomic sequencing in clinical trials
... With the vast amounts of high-quality, complex data now being processed through NGS, an ongoing challenge for translational researchers remains: How do we deal with the computational complexities of analyzing this data? ...
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Sequence analysis and editing for bisulphite genomic sequencing projects
... Bisulphite genomic sequencing is a widely used technique for detailed analysis of the methylation status of a region of ...of sequencing results (since the long runs of T or A residues are difficult ...
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Genotype-Specific Genomic Markers Associated with Primary Hepatomas, Based on Complete Genomic Sequencing of Hepatitis B Virus
... identify genomic markers in hepatitis B virus (HBV) that are associated with hepatocellular carcinoma (HCC) development by comparing the complete genomic sequences of HBVs among patients with HCC and those ...
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Genomic landscape of colorectal cancer in Japan: clinical implications of comprehensive genomic sequencing for precision medicine
... mutations in these genes cluster in “hot-spots” (i.e. KRAS codon 12, 13; NRAS codon 61; BRAF codon 600); however, data from large full-gene sequencing projects have identified additional mutations outside these ...
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Psychosocial Factors Influencing Parental Interest in Genomic Sequencing of Newborns
... Although these data were generated from a hypothetical survey, we are now engaged in assessing whether interest in GS will be similar when parents of newborns are presented with the real option of having their infant’s ...
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Perceived Benefits, Risks, and Utility of Newborn Genomic Sequencing in the BabySeq Project
... with genomic information and many times linked that to parents’ distress, suggesting that GS could cause unnecessary parental distress by causing parents to worry about health problems that may never arise or that ...
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Genomic sequencing and analyses of HearMNPV—a new Multinucleocapsid nucleopolyhedrovirus isolated from Helicoverpa armigera
... Comparative genomic analyses showed that overall gene content and arrangement in these four viruses were highly conserved, and they are considered variants of the same NPV species ...
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Variations in the cytomegalovirus major immediate early gene found by direct genomic sequencing
... An assay to detect and sequence DNA from human cytomegalovirus HCMV immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction [r] ...
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Clinical providers’ experiences with returning results from genomic sequencing: an interview study
... The potential for complex results requiring extensive preparation has been cited as an anticipated challenge of disclosing results for GS [25]. While initially some of the respondents noted these concerns, they were ...
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Overview of Genomic Tools for Circular Visualization in next-Generation Genomic Sequencing Era
... takes inputs as several types of genomic data formats and plots circular results [24]. All intermediate[r] ...
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Genomic Sequencing and Characterization of Cynomolgus Macaque Cytomegalovirus
... The newly characterized CyCMV (Ottawa strain) is 218,041 bp in length, encodes 262 ORFs, and is most closely related to the two published genomes of RhCMV (strains 68.1 and 180.92). Although we have predicted 262 ORFs, ...
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Genomic sequencing of a dyslexia susceptibility haplotype encompassing ROBO1
... low sequencing depth, the SNV call- ing program would regard technical errors as variants and yield false positive ...ual sequencing data, with over 50-fold average depth, compensated the weakness of low ...
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Genomic sequencing is required for identification of tuberculosis transmission in Hawaii
... whole-genome sequencing (WGS) provides a new tool for molecular epidemiology that can resolve clusters of isolates with identical spoligotyping and MIRU-VNTR ...
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