GTP/GDP
The GDP GTP exchange factor collybistin: an essential determinant of neuronal gephyrin clustering
... Inhibitory neurotransmission relies on glycine and γ-aminobutyric acid (GABA) receptors being clustered at postsynaptic specializations opposite axon terminals releasing the corresponding neurotransmitters. Central to ...
44
Activated Alleles of the Schizosaccharomyces pombe gpa2+ Gα Gene Identify Residues Involved in GDP-GTP Exchange
... are GDP-GTP exchange ...of GDP, the Gpa2 proteins were immediately assayed for GTP ␥ S binding (51), reflecting the rate of GDP release that is required to allow GTP ...fmol ...
8
Cdc24, the GDP-GTP Exchange Factor for Cdc42, Is Required for Invasive Hyphal Growth of Candida albicans
... albicans vegetative and hyphal growth, strains were constructed in which one copy of the CDC24 ORF was replaced by the HIS1 marker and the MET3 promoter was integrated immediately 5 ⬘ of[r] ...
10
A Role for the Rap GTPase YlRsr1 in Cellular Morphogenesis and the Involvement of YlRsr1 and the Ras GTPase YlRas2 in Bud Site Selection in the Dimorphic Yeast Yarrowia lipolytica
... the GTP- and GDP-bound states but its function in cellular morphogenesis does not, suggesting that the two processes are differentially ...The GTP/GDP cycling and the plasma membrane ...
11
eIF2B Bodies and their Role in the Control of Protein Synthesis
... FRAP assesses the catalytic activity of eIF2B by exploiting the movement of eIF2 into and out of the body, presumably for GDP-GTP exchange. As this is a novel method for potentially measuring in vivo GEF ...
222
A potential role for guanine nucleotide binding protein in the regulation of endosomal proton transport
... Both GTP and GTP-gamma-S stimulated significantly the initial rate of proton ...contrast, GDP-beta-S, which does not activate GTP binding proteins, inhibited proton ...was GTP-gamma-S ...
7
Glucose and GTP dependent stimulation of the carboxyl methylation of CDC42 in rodent and human pancreatic islets and pure beta cells Evidence for an essential role of GTP binding proteins in nutrient induced insulin secretion
... which GTP gS could ex- ert its stimulatory effects, since GTP stimulated the carboxyl methylation of CDC42 in the isolated secretory granule frac- ...of GTP gS to CDC42 aug- ments its affinity for ...
17
Regulation of RhoA GTPase signaling by guanine nucleotide exchange factors in response to extracellular matrix adhesion and DNA damage
... protein families: Guanine nucleotide exchange factors (GEFs) increase activity of Rho proteins by promoting the exchange of GDP for GTP [7], GTPase activating proteins (GAPs) decrease [r] ...
160
GTP-dependent run-up of Piezo2-type mechanically activated currents in rat dorsal root ganglion neurons
... contained GTP, We did not observe RA current run-up under the perforated patch-clamp re- cording ...when GTP was re- placed with GDP, suggesting that RA current run-up was specifically dependent on ...
8
Secretion and the actin cytoskeleton in permeabilised mast cells
... of GTP-y-S on most GTP-binding protein-regulated pathways, GTP-y-S actually inhibits Rab-mediated vesicle trafficking (but not vesicle formation) indicating that cycling between the GDP- and ...
193
Defining diverse mechanisms that regulate the activity of Dbl-family guanine nucleotide exchange factors
... As previously mentioned, GTPases of the Ran family are not lipid-modified and their function does not depend on their ability to associate with a cellular membrane [4]. Only one Ran GTPase, Ran, has been identified in ...
158
Saccharomyces cerevisiae Putative G Protein, Gtr1p, Which Forms Complexes With Itself and a Novel Protein Designated as Gtr2p, Negatively Regulates the Ran/Gsp1p G Protein Cycle Through Gtr2p
... putative GTP-bound mutants, gtr1-S15V and gtr1-S15G, 308, the glutathione Sepharose-4B beads were spun rescued the cold sensitivity of the gtr1-1D strain with an down, and the radioactivity that was coprecipitated ...
15
Synthesis, biological evaluation, and utility of fluorescent ligands targeting the μ opioid receptor
... of GDP is significantly higher than GTP, the receptor will exist predominantly in the low-affinity G protein uncoupled state state, which is reflected in the lower agonist affinities ...
50
CSGID solves structures and identifies phenotypes for five enzymes in Toxoplasma gondii
... Frontiers in Cellular and Infection Microbiology | www.frontiersin.org Catalyzes movement of phosphate from nucleoside triphosphate to nucleoside diphosphate GTP + ADP –> GDP + ATP Conve[r] ...
21
Daughter Cell Identity Emerges from the Interplay of Cdc42, Septins, and Exocytosis
... The interplay between the morphology of the septin ring and the cell shape has long attracted attention in the field (Caviston et al., 2003; Gladfelter et al., 2005). We observed that cells deleted for Cdc42 GAPs formed ...
14
Evidence of a role for GTP in the potentiation of Ca(2+) induced insulin secretion by glucose in intact rat islets
... nucleotides. A similar lack of effect of MPA on E2 was seen with a second mitochondrial fuel, alpha-ketoisocaproic acid (KIC). However, in the absence of diazoxide and K+, MPA blunted the secretory effects of either ...
12
Beyond GDP
... growing GDP, consumption and economic activity is leading the world towards increasing instability, natural resource depletion and environmental degradation, while developing nations still need to lift people from ...
32
TUFT1 promotes metastasis and chemoresistance in triple negative breast cancer through the TUFT1/Rab5/Rac1 pathway
... We next sought to determine whether TUFT1 confers resistance to chemotherapy in TNBC cells via the Rab5/ Rac1/NF-κB signaling pathway. Treatment of TUFT1- knockdown MDA-MB-231 cells with doxorubicin and taxotere induced ...
14
A Nucleotide Binding Motif in Hepatitis C Virus (HCV) NS4B Mediates HCV RNA Replication
... Over 150 million people are infected with hepatitis C virus (HCV) worldwide (1). Current therapies are inadequate for most of these individuals (18). HCV is a positive single- stranded RNA virus. Its 9.6-kb genome ...
8
Diagnosing Channel Issues using GTP Protocol Messages in LTE Core Networks
... The situation is more complex in 3G. The source IP address no longer belongs to the NodeB but to the RNC which controls a set of NodeBs. Communication between an RNC and its child NodeBs are not seen in the core network. ...
6