Haloferax volcanii
Two Distinct N Glycosylation Pathways Process the Haloferax volcanii S Layer Glycoprotein upon Changes in Environmental Salinity
... ABSTRACT N-glycosylation in Archaea presents aspects of this posttranslational modification not seen in either Eukarya or Bac- teria. In the haloarchaeon Haloferax volcanii, the surface (S)-layer ...
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Co expression and purification of the RadA recombinase with the RadB paralog from Haloferax volcanii yields heteromeric ring like structures
... Keywords: Homologous recombination, RadA, Haloferax volcanii, Co-expression, Halophilic protein purification... Abbreviations: Immobilized metal affinity chromatography IMAC; Discrete Op[r] ...
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RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator
... Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since ...
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Genetic and biochemical identification of a novel single stranded DNA binding complex in Haloferax volcanii
... Single-stranded DNA (ssDNA)-binding proteins play an essential role in DNA replication and repair. They use oligonucleotide/oligosaccharide-binding (OB)-folds, a five-stranded β-sheet coiled into a closed barrel, to bind ...
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Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii
... The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios ...
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Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support
... The alcohol dehydrogenase from Haloferax volcanii HvADH2 utilises NADPH β- nicotinamide adenine dinucleotide phosphate as a co-factor with some activity with NADH-nicotinamide adenine di[r] ...
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Dunaliella salina and Haloferax volcanii Synergistically Attenuate Skin Cancer in Vitro
... The cancer cell lines were treated without or with the maximal dose of Dunaliel- la salina and Haloferax volcanii that did not reduce the cell’s viability. After 24 hr, the cells were harvested and 50,000 ...
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Evolution of Genome Architecture in Archaea : Spontaneous Generation of a New Chromosome in Haloferax volcanii
... in Haloferax volcanii, we focussed on the four orc genes, orc1, orc5, orc2 and orc3, which are genetically linked to the four chromosomal origins, oriC1, oriC2, oriC3 and ori-pHV4, respectively (Figure ...
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DNA damage induces nucleoid compaction via the Mre11 Rad50 complex in the archaeon Haloferax volcanii
... Haloferax volcanii is a halophilic archaeon, growing optimally at salt concentrations of ...H. volcanii contains one main chromosome and three mega-plasmids (Hartman et ...H. volcanii is ...
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Genetic Identification of Three ABC Transporters as Essential Elements for Nitrate Respiration in Haloferax volcanii
... More than 40 nitrate respiration-deficient mutants of Haloferax volcanii belonging to three different phenotypic classes were isolated. All 15 mutants of the null phenotype were complemented with a genomic ...
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Structural and functional adaptation of Haloferax volcanii TFEα/β
... from Haloferax volcanii (Hvo) does encode a bona fide TFE  subunit, which does not encompass an FeS cluster and yet still forms a stable complex with TFE ␣ ...the Haloferax tfeB gene is not ...
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Haloferax Volcanii Strategies to Regulate Type IV Pilus Dependent Adhesion and Microcolony Formation
... archaeon Haloferax volcanii employs to permit effective adhesion and microcolony formation, early steps in biofilm formation and maturation, as well as eventual dispersal from ...
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Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery
... H. volcanii DNA, the probability (P) at which every gene is targeted at least once can be calculated using the formula P = 1 - (1 - x/G) n [68], where x is the average gene size, G is the genome size, and n is the ...
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Co expression and purification of the RadA recombinase with the RadB paralog from Haloferax volcanii yields heteromeric ring like structures
... binding buffer with increasing concentration of the RadA-RadB protein complex 0-8 μM did.. not result in protein-DNA complex formation on either 10% or 7% polyacrylamide..[r] ...
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A novel archaeal DNA repair factor that acts with the UvrABC system to repair mitomycin C induced DNA damage in a PCNA dependent manner
... Fig. 1. A. Schematic representation of thirteen Nre proteins from nine diverse archaeal species showing the presence or absence of the N-terminal C4 metal binding domain (gray box) and C-terminal PCNA binding motif (PIP ...
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Biofilms formed by the archaeon Haloferax volcaniiexhibit cellular differentiation and social motility, and facilitate horizontal gene transfer
... of Haloferax volcanii ...H. volcanii H1206 under transmitted light (left), under blue excitation (center), and by the strain ...H. volcanii H1206(pJAM1020) under blue excitation ...H. ...
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Identification of essential and non essential single stranded DNA binding proteins in a model archaeal organism
... Single-stranded DNA-binding proteins (SSBs) play vital roles in all aspects of DNA metabolism in all three domains of life and are characterized by the presence of one or more OB fold ssDNA-binding domains. Here, using ...
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Protein phosphorylation and its role in archaeal signal transduction
... Despite the fact that TCS genes have been identified in Eur- yarchaeota, their physiological role has not been studied in great detail (Bourret and Silversmith 2010). Studies so far only include TCS in halophilic ...
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Novel Cell Surface Anchoring Mechanism Of Prokaryotic Secreted Protein
... mass spectrometric analyses of S-layer proteins have failed to identify peptides at the carboxy-terminus of the protein, which was taken as evidence that the transmembrane segment may not be present in the mature protein ...
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Utilization of vinasse for production of poly-3-(hydroxybutyrate-co-hydroxyvalerate) by Haloferax mediterranei
... genera Haloferax, Haloarcula, Haloquadratum, Haloterrigena, Halorhabdus, Halobi- forma and Halopiger are found to accumulate ...PHAs. Haloferax mediterranei is so far the best PHA producer of the family ...
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