HCT116 Cells
Original Article Effect of overexpression of activating transcription factor 3 on biological behaviors of human colorectal cancer HCT116 cells
... In colorectal cancer, the potential role of ATF3 in metastasis may depend on cell genetic back- ground [21]. In the current study, our findings confirmed that ATF3 overexpression could sup- press the migration and ...
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Original Article BC200 RNA is over-expressed in colorectal cancer and promotes migration and invasion of HCT116 cells
... 5% HCT116 cells were plated to each well of 12-well plates at least 24 h before transfection to achieve 70-80% ...instructions. Cells were collected after transfection for RNA isolation, MTS cell ...
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Inhibitory effects of adenovirus mediated tandem expression of RhoA and RhoC shRNAs in HCT116 cells
... Collected cells were seeded onto 96-well microplates and cellular growths were determined by a continuous 6-day MTT ...the HCT116 cells infected with Ad-A1+A2+C1+C2 and the other two groups was not ...
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Original Article Osthenol inhibits the viability of HCT116 cells through suppressing PI3K/AKT signaling pathway
... promote HCT116 cells apoptosis in a concentration dependent ...in HCT116 were significant enhanced after treated with ...decrease HCT116 cells migration and arrest cell cycle in G1 ...
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In Vitro Anticancer Activity Of Bark, Flower And Leaves Extracts Of Simarouba Glauca On Hct116 Cells.
... on HCT116 cell lines by MTT assay with camptothecin as a ...of HCT116 cells with bark extracts from ethanol, methanol and water at 450 μg/ml demonstrated complete cell ...
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Action of free and polymer carrier encapsulated doxorubicin towards HCT116 cells of human colorectal carcinoma
... more intensive cleavage of the reparation enzyme PARP and longer delay in G2/M cell cycle ar- rest, compared to such effects of free doxorubicin. The developed carrier itself is non-toxic to the used mammalian ...
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Temozolomide analog PMX 465 downregulates MGMT expression in HCT116 colorectal carcinoma cells
... in HCT116 cells after treatment (48 h) with TMZ (50 μM), PMX 465 (50 μM) and O6-BG (10 ...6A). HCT116 cells were then exposed to TMZ (50 μM) and PMX 465 (20, 35 and 50 μM) for 48 ...in ...
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Hypoxia regulates TRAIL sensitivity of colorectal cancer cells through mitochondrial autophagy
... DLD1 cells grown under hypoxic conditions using the membrane potential-independent MitoTracker dye revealed a reduced staining intensity compared to normoxic controls (Figure ...and HCT116 cells ...
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Increased expression of fatty acid synthase provides a survival advantage to colorectal cancer cells via upregulation of cellular respiration
... in HCT116 cells with stable knockdown of FASN, as compared to control, is primarily due to a decrease in non-mitochondrial respiration of cells; however, knockdown of FASN in HT29 cells ...
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Inhibition of telomerase activity by HDV ribozyme in cancers
... bel7402, HCT116 cells (5 × 10 4 ) were seeded in 6-well plates, a day before ...7402 cells, colon cancer cells HCT116 and normal human heptaocyte L02 with both the 10 μg ...all ...
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Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells
... in HCT116 cells only; no significant changes were found in WI-38 ...WI-38 cells; however, the original levels were restored after 48 ...in HCT116 cells or the levels of CENP-A in either ...
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Global loss of DNA methylation uncovers intronic enhancers in genes showing expression changes
... DKO1 cells and one replicate of RNAPII ChIP-seq was performed in HCT116 cells, as previously described ...from HCT116 were available via ENCODE and were downloaded from the UCSC browser, ...
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miR-30e controls DNA damage-induced stress responses by modulating expression of the CDK inhibitor p21 WAF1/CIP1 and caspase-3
... However, the here observed p21 upregulation is clearly not mediated by a direct binding of miR-30e to the p21-mRNA, as real-time PCR analyses of 3’-UTR-p21- mRNA immunoprecipitates confirmed only the presence of miRNAs ...
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A novel HSP90 inhibitor with reduced hepatotoxicity synergizes with radiotherapy to induce apoptosis, abrogate clonogenic survival, and improve tumor control in models of colorectal cancer
... tumor cells [17, ...irradiation. HCT116 cells were pretreated with NW457, irradiated at 5 Gy, and microscopical examination of nuclei was performed 24-72 h after ...
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Original Article Downregulation of NLRP12 enhances the proliferation, migration and drug-resistance of colorectal cancer cells by modulating MEK/ERK/GLI1 signaling pathway
... and cells. Later NLRP12 siRNA was transfected into HCT116 cells to explore the effects of NLRP12 on the proliferation, migration and drug-resistance in colorectal cancer cells in ...in ...
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Metastasis regulation by PPARD expression in cancer cells
... PPARD-KO HCT116 cells were 87% lower than those in parental WT HCT116 cells and that PPARD reconstitution in PPARD-KO cells reversed IL-8 downregulation (Figure ...by HCT116 ...
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The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway
... cancer cells apoptosis upon treatment of NKP608, we assessed apop- tosis by Annexin V- FITC/PI staining and flow cytom- eter ...in HCT116 cells, in addition, the enzymatic activ- ity of Caspase-3 was ...
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Chansu inhibits the expression of cortactin in colon cancer cell lines in vitro and in vivo
... cancer cells (HCT116 and HT29) with CBF (1 ...cancer cells were also exposed to hypoxic and normoxic conditions, respectively ...in HCT116 cells under 1 % ...in HCT116 ...
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Dissecting mechanisms of resistance to targeted drug combination therapy in human colorectal cancer.
... parent HCT116 cells to com- bination treatments for a prolonged period we generated cell lines that had lost the synergistic response that was initially exhibited to the MEK – PI3K combination, and ...
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Absence of p300 induces cellular phenotypic changes characteristic of epithelial to mesenchyme transition
... p300 cells (Figure ...p300 cells (Figure 2A): decreased staining of b-catenin and ZO-1 at cell – cell junctions and increased nuclear b-catenin staining (Figure ...p300 cells in comparison to the WT ...
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