HCV quasispecies
Differing Patterns of Liver Disease Progression and Hepatitis C Virus (HCV) Quasispecies Evolution in Children Vertically Coinfected with HCV and Human Immunodeficiency Virus Type 1
... seen. HCV RNA was extracted from plasma and was amplified using previously described primers and conditions (8, ...of HCV quasispecies starting at ...
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Analysis of HCV quasispecies dynamic under selective pressure of combined therapy
... The follow-up of patients’ samples showed that genetic diversity of populations decreased over time. Post-therapy population of end-of-treatment responders presented higher genetic distance from baseline probably due to ...
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Complexity and Catalytic Efficiency of Hepatitis C Virus (HCV) NS3 and NS4A Protease Quasispecies Influence Responsiveness to Treatment with Pegylated Interferon plus Ribavirin in HCV/HIV-Coinfected Patients
... the HCV treatment response, we analyzed the pretreatment NS3/4A protease gene quasispecies composition of 56 HCV genotype 1–HIV-1-coinfected patients treated in our clinic with pegylated IFN (pegIFN) ...
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Multigene Tracking of Hepatitis C Virus Quasispecies after Liver Transplantation: Correlation of Genetic Diversification in the Envelope Region with Asymptomatic or Mild Disease Patterns
... of HCV quasispecies after liver trans- ...of HCV quasispecies in six representative ...with HCV genotype 1 infection who fell into one of three disease categories were selected for ...
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Correlation between pre treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a
... Samples in the SVR group showed a decrease in HD, GD, Sn-nt and Sn-aa between the B sample and the other serial samples available for analysis but none of the difference were significant (Table 2). These variations were ...
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Understanding how ESOL Pre Services Teachers' Prior Experiences and Background Shape their Processes of becoming L2 (Reading) Teachers
... among quasispecies sequences and relatively high sequencing error rate in data generated by current ...among quasispecies sequences helps to identify more sequencing errors as “outliers” and easier ...
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Wide Range of Quasispecies Diversity during Primary Hepatitis C Virus Infection
... of HCV quasispecies in 12 seronegative subjects with primary infection following community exposures, six acutely infected recipients of HCV-seropositive blood transfusions and six seropositive ...
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Accurate Representation of the Hepatitis C Virus Quasispecies in 5 2 Kilobase Amplicons
... of HCV quasispecies during acute infection by using a method for amplifying most of the viral genome from human serum by nested RT-PCR at a level of sensitivity that makes it practical for studying acute ...
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Vertical Transmission of Hepatitis C Virus: Variable Transmission Bottleneck and Evidence of Midgestation In Utero Infection
... particular HCV variants. Here we report results of a longitudinal assessment of HCV quasispecies diversity and composition in 5 cases of vertical HCV transmission, including 3 women coinfected ...
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HCV core antigen and HCV RNA in HIV/HCV co infected patients with different HCV genotypes
... in HCV monoinfected patients as indicated by previous studies ...of HCV antigen in HIV/HCV ...the HCV Core gene sequences and a more diversified HCV quasispecies population in ...
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Determining the Cellular Diversity of Hepatitis C Virus Quasispecies by Single-Cell Viral Sequencing
... virus quasispecies diversity at the cellular level utilizing hepatitis C virus (HCV) ...JFH-1 HCV replicons constitutively expressed in Huh7 ...unique quasispecies that was much less diverse ...
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Effectiveness of HCV core antigen and RNA quantification in HCV infected and HCV/HIV 1 coinfected patients
... of HCV- RNA in HIV plasma may partly account for the higher Ag/RNA ratios in HCV/HIV-1-coinfected patients with lower CD4+ T-cell ...5, HCV/HIV-1-coinfected patients with an im- paired immune system ...
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Effect of Hepatitis C Virus (HCV) NS5B-Nucleolin Interaction on HCV Replication with HCV Subgenomic Replicon
... necessary to show this in isolate M1LE. Due to the poor recovery of soluble full-length NS5B, we utilized NS5Bt (68), a soluble form of NS5B in which the C-terminal 21 aa were truncated, to dissect the interaction ...
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An Automated Immunoblot Method for Detection of IgG Antibodies to Hepatitis C Virus: a Potential Supplemental Antibody Confirmatory Assay
... of HCV infection, a second anti-HCV test is necessary to confirm the first anti-HCV-positive test ...or HCV Blot ...generation HCV antigen-containing core and nonstructural proteins NS3, ...
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Seronegative Hepatitis C Virus Infection in a Child Infected via Mother to Child Transmission
... positive HCV RNA PCR tests (Cobas Amplicor HCV, version ...for HCV RNA at ...(AxSYM HCV, version ...and HCV ELISA [en- zyme-linked immunosorbent assay] test system, version ...INNO-LIA ...
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Effect of combined siRNA of HCV E2 gene and HCV receptors against HCV
... of HCV cell entry was unrevealed after a long ...studied HCV replication in serum infected liver cell lines which mimics the naturally occur- ring HCV virions biology and kinetics of HCV ...
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Is the Quasispecies Concept Relevant to RNA Viruses?
... the quasispecies that it is fre- quently cited whenever sequence polymorphism is encoun- tered in viral populations and used to explain an enormous array of evolutionary observations (5, 6, 11, ...
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Discovering Complete Quasispecies in Bacterial Genomes
... our quasispecies model only estimates ef- fective duplication rates, which is the fraction of duplications that do not incur a significant fitness cost to the host and are therefore selected against at the level of ...
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Generation and Characterization of Chimeric Antibodies against NS3, NS4, NS5, and Core Antigens of Hepatitis C Virus
... for HCV infection include serological assays for antibodies and molecular techniques for detecting virus ...Current HCV immunoassay QC re- agents are derived from HCV-infected patient serum and/or ...
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Probability of Fixation of an Advantageous Mutant in a Viral Quasispecies
... viral quasispecies is investigated in the framework of multitype branching ...the quasispecies that will form if invasion is successful rather than on the individual fitness of the invading ...invading ...
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