Heat-labile enterotoxin
Expression of Escherichia coli heat labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L ) fruit
... We report a feasibility study for expressing the LTB protein (Escherichia coli heat-labile enterotoxin B subunit) via Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.). We ...
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Preparation of biocompatible heat-labile enterotoxin subunit B-bovine serum albumin nanoparticles for improving tumor-targeted drug delivery via heat-labile enterotoxin subunit B mediation
... Abstract: Heat-labile enterotoxin subunit B (LTB) is a non-catalytic protein from a pentameric subunit of Escherichia coli. Based on its function of binding specifically to ganglioside GM1 on the ...
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Plasmid Vectors Encoding Cholera Toxin or the Heat-Labile Enterotoxin from Escherichia coli Are Strong Adjuvants for DNA Vaccines
... coli heat-labile enterotoxin (LT) were evaluated for their ability to serve as genetic adjuvants for particle-mediated DNA vaccines administered to the epidermis of laboratory ...
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Human Papillomavirus Virus-Like Particles Are Efficient Oral Immunogens when Coadministered with Escherichia coli Heat-Labile Enterotoxin Mutant R192G or CpG DNA
... coli heat-labile enterotoxin (LT) mutant R192G (LT R192G) or CpG DNA can significantly improve anti-VLP humoral responses in peripheral blood and in genital mucosal ...
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Heat Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons
... ever, each CT family member variant has unique biological properties that may warrant development as therapeutic platforms. In the current study, a nontoxic variant of the heat-labile enterotoxin IIa ...
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Specificity of Escherichia coli Heat-Labile Enterotoxin Investigated by Single-Site Mutagenesis and Crystallography
... the heat-labile enterotoxin (LT), a close homologue of the cholera ...the heat-labile enterotoxin with surface plasmon resonance spectroscopy and compared the data to the parent ...
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Improved GM1 enzyme linked immunosorbent assay for detection of Escherichia coli heat labile enterotoxin
... Previously described GM1 ganglioside enzyme-linked immunosorbent assays GM1-ELISA for the detection of Escherichia coli heat-labile enterotoxin LT showed sensitivity equal to the Y-1 adr[r] ...
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CONSTRUCTION OF YEAST EXPRESSION VECTOR CONTAINING ELT-B GENE FOR HIGH LEVEL OF HEAT LABILE ENTEROTOXIN-B SUBUNIT (LT-B)
... The heat labile enterotoxin (LT) from E.coli is a multimeric protein consisting of a 27 KDa subunit A (LT-A) and a pentamer of 11.6KDa subunit B (LT-B). LT-B specifically binds to the GM1 ...
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Detection of heat labile enterotoxin producing colonies of Escherichia coli and Vibrio cholerae by solid phase sandwich radioimmunoassays
... A solid-phase sandwich assay that was able to differentiate heat-labile-enterotoxin-producing colonies of Escherichia coli and choleratoxin-producing colonies of Vibrio cholerae from non[r] ...
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Functional Pentameric Formation via Coexpression of the Escherichia coli Heat-Labile Enterotoxin B Subunit and Its Fusion Protein Subunit with a Neutralizing Epitope of ApxIIA Exotoxin Improves the Mucosal Immunogenicity and Protection against Challenge by Actinobacillus pleuropneumoniae
... coli heat-labile enterotoxin B subunit (LTB) and a fusion protein of an ApxIIA toxin epitope produced by Actinobacillus pleuropneumoniae coupled to LTB, respectively, was adapted for the ...
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Rapid test for identification of heat labile enterotoxin producing Escherichia coli colonies
... A latex particle agglutination test is described which is suitable for the recognition of heat-labile enterotoxin-producing colonies of Escherichia coli immediately after primary culture[r] ...
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Gangliosides sensitize unresponsive fibroblasts to Escherichia coli heat labile enterotoxin
... exogenous mono-, di-, and trisialogangliosides. After the uptake of monosialoganglioside galactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl]-galactosylglucosylceramide (GM1), the cells responded to E. coli ...
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GM1 erythroimmunoassay for detection and titration of Escherichia coli heat labile enterotoxin
... Method for the detection and dosage of the Escherichia coli heat-labile enterotoxin by an enzyme-linked immunosorbent assay using a new simple kit test.. Production of "Escherichia coli"[r] ...
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Oral Administration Recombinant Bifidobacterium LTB (B Subunit of Heat Labile Enterotoxin) Enhances the OVA (Ovalbumin) Specific sIgA in Jejunal Mucosa of Sprague Dawley (SD) Rat
... The LTB of Escherichia coli is one of bacterial products with the greatest potential to function as mucosal adju- vant. LTB belongs to ADP-ribosylating enterotoxins (cholera toxin and the heat-labile ...
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Simultaneous detection of Escherichia coli heat stable and heat labile enterotoxin genes with a single RNA probe
... A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains.. The resu[r] ...
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Rapid GM1 enzyme linked immunosorbent assay with visual reading for identification of Escherichia coli heat labile enterotoxin
... All the cultures of the strains previously shown to produce LT had a significant enterotoxin titer as determined with the GM1-AP-ELISA and also.. the cultures to a final concentration of[r] ...
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Solid Phase Microtiter Radioimmunoassay Blocking Test for Detection of Antibodies to Escherichia coli Heat Labile Enterotoxin
... Again the actual antibody titer of these sera was considerably higher in the RIA blocking test than the adrenal cell neutralization assay; however, the relative correlation of the two te[r] ...
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Comparison of methods to detect Escherichia coli heat labile enterotoxin in stool and cell free culture supernatants
... In addition, the ELISA of stool supernatants detected more samples known to be positive by YAC assay of pooled culture supernatants than did the ELISA of culture supernatants, possibly o[r] ...
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Automatic and manual latex agglutination tests for measurement of cholera toxin and heat labile enterotoxin of Escherichia coli
... M glycine-NaOH buffer This paper deals with automatic and manual Dow latex suspension inM0.05 8.2 containing 0.05 sodium chloride was mixed latex agglutination tests for cholera toxin CT[r] ...
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Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing Escherichia coli
... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ...
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