HEK 293T cells
Development of a Model System for Tick Borne Flavivirus Persistence in HEK 293T Cells
... kidney) cells differed from our LGTV system in that cells surviving the initial lytic crisis reattained con- fluence but underwent successive lytic crises ...for HEK 293T cells, ...
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Transcriptome Analysis Reveals a Signature Profile for Tick Borne Flavivirus Persistence in HEK 293T Cells
... (HEK) 293T cells in vitro results in the death of >95% of infected cells by day ...surviving cells to repopulate and become persistently infected for extended peri- ods of ...the ...
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S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T Cells
... Human Reelin containing 5th and 6th repeats (R5-R6), a part of 8 th repeat and the CTR which were amplified from cDNA of K562 and HEPG2 cells and cloned into a mammalian expressional plasmid (pVP22/myc-His). ...
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Incorporation of podoplanin into HIV released from HEK 293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC 2
... viable cells were ana- lyzed (see below), indicating that HIV particles generated in patients might not harbour ...tumour cells promotes hematogenous tumour metastasis [71,74], possibly by inducing growth ...
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Cell- and Protein-Directed Glycosylation of Native Cleaved HIV-1 Envelope
... in HEK 293T cells failed to accurately repro- duce the highly processed complex-type glycan structures observed on PBMC-derived gp120, and in particular the precise link- age of sialic acid residues ...
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Peste des Petits Ruminants Virus Nucleocapsid Protein Inhibits Beta Interferon Production by Interacting with IRF3 To Block Its Activation
... further. HEK-293T cells were transfected with Flag-N- encoding plasmids and then infected with ...and cells were collected separately for the enzyme-linked immunosorbent assay (ELISA) and ...
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HIV-1 Vpr Redirects Host Ubiquitination Pathway
... checked. HEK 293T cells were cotransfected with His-Ub and UNG2-HA, infected with pNL4-3/pNL4-3 ⌬ vpr VSV-G-pseudotyped virus after 12 h, and treated with MG132 after 36 h for 8 ...infected ...
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Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Antagonizes Beta Interferon Production by Sequestering the Interaction between IRF3 and TBK1
... production, HEK-293T cells were transfected with a DNA expres- sion construct encoding the HA-tagged N protein and then in- fected with ...The cells and supernatants were collected sepa- ...
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Improved fusion protein expression of EGFP via the mutation of both Kozak and the initial ATG codon
... on cells, the cDNA sequence of BmKK2 (a short-chain potassium- channel scorpion-toxin gene) [7, 8] was inserted into the XhoI-HindIII cut of pEGFP-N1 to form a toxin-EGFP fusion ...after HEK 293T ...
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Middle East Respiratory Syndrome Coronavirus Accessory Protein 4a Is a Type I Interferon Antagonist
... in HEK-293T ...in HEK-293T cells, fixed after 24 h, and stained with a mouse anti-FLAG immunoglobulin G (IgG) for immunofluorescence assay analysis by confocal microscopy (Leica, SP5 ...
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Foamy Virus Nuclear RNA Export Is Distinct from That of Other Retroviruses
... of HEK 293T cells with pcHSRV2 and an enhanced green fluorescent protein (EGFP)-encoding control plasmid (pEGFP), cells were incu- bated for 16 h with medium containing 10 nM ...LMB-treated ...
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A novel two step genome editing strategy with CRISPR Cas9 provides new insights into telomerase action and TERT gene expression
... The protocol was first tested using HEK 293T cells, which contain WT TERT promoter sequences. Ten sgRNA sequences were tested, and a sequence directing Cas9 to cut between the −148 and −147 positions ...
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The HDAC6/APOBEC3G complex regulates HIV 1 infectiveness by inducing Vif autophagic degradation
... in cells producing virions and HIV-1 infec- tiveness, regardless viral ...in HEK 293T cells overexpressing wt-HDAC6, where Vif is degraded and A3G subsequently ...in HEK 293T ...
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Accuracy estimation of foamy virus genome copying
... in HEK 293T cells by Western blotting ...these cells by Western blotting (data not ...these cells, whereas HeLa cells were negative for APOBEC message ...in 293T ...
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Original Article DSTYK knockout affects the architecture of F-actin cytoskeleton in mouse renal proximal convoluted tubules
... in HEK 293T cells and performed a Co-IP assay using FLAG M2 agarose ...co-transfected cells (Figure ...non-muscle cells [18], we also examined whether Profilin 1 inter- acts with ...
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The G-Patch Domain of Mason-Pfizer Monkey Virus Is a Part of Reverse Transcriptase
... Western blot analysis of the concentrated M-PMV stock using anti-GPD antibody revealed the presence of not only PR17 but also a protein with an approximate molecular mass of 50 kDa (p50-GPD). This p50-GPD protein was ...
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The effect of simvastatin on lipid droplets accumulation in human embryonic kidney cells and pancreatic cancer cells
... MiaPaCa-2 cells was in general higher as compared to that in HEK-293T cells (Figure 2a ...MiaPaCa-2 cells there were not detectable changes in LDs amounts after 24 hours of ...MiaPaCa-2 ...
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West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5
... in HEK-293T cells transfected with empty vector or NS1 expression plasmid and treated or not treated with SeV (A) or poly(I·C) (B) for 8 h after ...in HEK-293T cells transfected ...
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Development of a tetracycline inducible lentiviral vector with an instant regulatory system : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science (MSc) in Biochemistry at Massey University, Manawatu, New Zealand
... into HEK 293T cells by infection, the transgene is integrated into the cell genome, allowing it to be stably ...transfected cells could not be reproduced every time in lentivirally transduced ...
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A human embryonic kidney 293T cell line mutated at the Golgi mannosidase II locus
... (HEK) 293T cell line, called Lec36, that displays sensitivity to ricin that lies between the parental HEK 293T cells, whose secreted and membrane-expressed proteins are dominated by ...
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