HEK293T cells
COG Complex Complexities : Detailed Characterization of a Complete Set of HEK293T Cells Lacking Individual COG Subunits
... KO cells labeled with GNL-647 revealed a uniform, bright plasma membrane staining that was distinct from control HEK293T cells (Figure ...KO cells with high-fidelity ...
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Antioxidant activity of Bios p peptide analogue in HEK293T cells and three dimensional structure prediction
... that HEK293T cells by incubating for 24 h with Bios-p shown a significant decreased of basal ex- tracellular ROS on total cell population in ...when cells are subjected to oxidative stress induced by ...
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Point mutations of the mTOR-RHEB pathway in renal cell carcinoma
... To assess the role of a point mutation in RHEB at the conserved Tyr35 site, we generated a point mutation (Y35N) and transiently over expressed wild- type and mutant RHEB in HEK293T cells. As RHEB is a key ...
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Arterivirus and Nairovirus Ovarian Tumor Domain-Containing Deubiquitinases Target Activated RIG-I To Control Innate Immune Signaling
... Vero cells, we have shown that the absence of MAVS, a central factor in RLR signaling, almost completely abrogated IFN-  expression, indicating that in these cells MAVS-mediated signaling is pivotal in the ...
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MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
... constructed. HEK293T cells were transfected with the indicated plasmids, and co-immunoprecipitation was performed to identify the interacting domains of MICAL2 and ...
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Issues associated with assessing nuclear localization of N-terminally unphosphorylated β-catenin with monoclonal antibody 8E7
... these cells. To address this, we subjected HEK293T cells to Wnt pathway activation using lithium chloride as previously described [8], followed by immunofluorescence double-labeling with the antibody ...
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PTPN4 negatively regulates CrkI in human cell lines
... in HEK293T cells and measured the phosphorylation status of CrkI in ...PTPN4-transfected cells was significantly reduced compared to the control cells ...in cells, we transfected CrkI ...
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The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
... dendritic cells (15), other cell types, such as lung epithelial cells, employ partially redundant recognition mechanisms and that virus-induced type I interferon through the RIG-I/TRIM25-dependent pathway ...
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Immunogold electron microscopic evidence of in situ formation of homo and heteromeric purinergic adenosine A1 and P2Y2 receptors in rat brain
... Dissected brain tissues were cut into 1.0 mm 3 blocks that were then incubated with lead (II) acetate (Sigma- Aldrich) buffer for 1 h at room temperature, dehydrated through a series of graded ethanol, and embedded in ...
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A Host-Oriented Inhibitor of Junin Argentine Hemorrhagic Fever Virus Egress
... (A) HEK293T cells were transfected with either JUNV Z-WT or JUNV-Z- ⌬ PTAP plasmids, and cell extracts and VLPs were harvested at 24 h ...in cells and VLPs. A Western blot control for  -actin in ...
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NOTCH pathway inactivation promotes bladder cancer progression
... T24 cells were cotrans- fected with a plasmid carrying a luciferase reporter gene directed by an RBPJ-responding promoter and with constructs carrying the indicated versions of NOTCH1 (N1) and NOTCH2 ...to ...
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A Molecular Sensor To Characterize Arenavirus Envelope Glycoprotein Cleavage by Subtilisin Kexin Isozyme 1/Site 1 Protease
... in HEK293T and HeLa ...hamster cells. The indicated sensors were transfected into HEK293T, CHO-K1, and SKI-1/S1P-deficient SRD12B ...transfected HEK293T cells sampled at the indicated ...
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Comparative Transcriptomics Highlights the Role of the Activator Protein 1 Transcription Factor in the Host Response to Ebolavirus
... While this study was largely comparative in nature, we focused much of our downstream attention on examining the role of AP1 in EBOV infection of human cells. We chose to focus on AP1 because the gene for its ...
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LDN 73794 Attenuated LRRK2 Induced Degeneration in a Drosophila Parkinson’s Disease Model
... In this study, characterization of a LRRK2 kinase inhibitor, LDN-73794, demonstrated that it reduced LRRK2 autophosphorylation at Ser2032 in HEK293T cells and protected against mutant G2[r] ...
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Original Article Mechanism underlying synergic activation of Tyrosinase promoter by MITF and IRF4
... B. HEK293T cells were transiently transfected with the TYR luciferase reporter and full length or truncated IRF4 expression plasmids including with ...
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e82.full.pdf
... apoptotic cells is not affected by amino acid substitutions in DBA MERTK, but is modulated by the amount of ...in HEK293T (human embryonic kidney 293T) cells and sMER in the conditioned medium ...
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Viperin Restricts Zika Virus and Tick-Borne Encephalitis Virus Replication by Targeting NS3 for Proteasomal Degradation
... T cells were transfected with FLAG-tagged TBEV prM, E, NS2A, or NS2B along with HA-NS3 in the presence of viperin or empty vector ...The cells were fixed 24 h posttransfection and stained, and fluorescent ...
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R spondin 2 is required for normal laryngeal tracheal, lung and limb morphogenesis
... untransfected HEK293T cells and cell bound AP activity ...(E) HEK293T cells co-transfected with TOPFLASH and pRL-TK were exposed to the indicated conditioned ...into HEK293T ...
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The pseudomonas aeruginosa Type VI secretion PGAP1 like effector induces host autophagy by activating endoplasmic reticulum stress
... It is known that ER stress induces the binding of chaperones Bip/ Grp78 to misfolded proteins and thus initiates the UPR (Hetz, 2012). We therefore hypothesized that TplE-dependent ER disruption would also result in UPR ...
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Role of LAMP1 Binding and pH Sensing by the Spike Complex of Lassa Virus
... confluent HEK293T cells were exposed to the indicated pH values for 5, 10, and 15 ...the cells are ...of HEK293T cell lysates for cells transfected with the various WT and mutated LASV ...
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