Helix bundle
Stabilizing the Native Trimer of HIV-1 Env by Destabilizing the Heterodimeric Interface of the gp41 Postfusion Six-Helix Bundle
... six-helix bundle-de- stabilizing mutants (gp160-V570D and gp160-I573D) bound neutralizing antibody b12 more efficiently than the nonneutraliz- ing antibody b6 ( ⬃ 5- to 6-fold difference) ...
15
The Cytoplasmic Tail Slows the Folding of Human Immunodeficiency Virus Type 1 Env from a Late Prebundle Configuration into the Six-Helix Bundle
... Effects of the cytoplasmic tail (CT) of human immunodeficiency virus type 1 Env on the process of membrane fusion were investigated. Full-length Env (wild type [WT]) and Env with its CT truncated ( ⌬ CT) were expressed ...
10
Structure and Mutagenesis of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Stalk Domain Reveals a Four-Helix Bundle and the Role of the Stalk in Fusion Promotion
... Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (hemagglu- tinin-neuraminidase [HN], H, or G). The multifunctional HN protein of some paramyxoviruses, besides functioning as ...
12
Resistance to N peptide fusion inhibitors correlates with thermodynamic stability of the gp41 six helix bundle but not HIV entry kinetics
... Increased bundle stabil- ity would favor the later peptide-insensitive conformation and thus fusion in the presence of ...increasing bundle stability could increase the rate of 6HB formation and shorten the ...
12
The Six-Helix Bundle of Human Immunodeficiency Virus Env Controls Pore Formation and Enlargement and Is Initiated at Residues Proximal to the Hairpin Turn
... the bundle is initiated by binding of the HR2 residues closest to the hairpin into the portion of the HR1 grooves that are also adjacent to the turn ...for bundle formation than residues that bind subse- ...
10
Common principles and intermediates of viral protein mediated fusion: the HIV 1 paradigm
... In spite of structural differences, different classes of fusion proteins appear to promote membrane merger through a common "cast-and-fold" mechanism (reviewed in [1- 6,11,16,22,23,61]). The critical evidence ...
13
Role of the Simian Virus 5 Fusion Protein N-Terminal Coiled-Coil Domain in Folding and Promotion of Membrane Fusion
... Cell surface expression analyzed by flow cytometry. To study the effect of these mutations in the context of the entire SV5 F protein, site-directed mutagenesis was employed to create the mutants SV5 F I137M, SV5 F ...
9
A Stabilized Headless Measles Virus Attachment Protein Stalk Efficiently Triggers Membrane Fusion
... 4-helix bundle tetramerization domains and demonstrate restored cell surface expression, efficient interaction with F, and fusion pro- motion activity of these ...4-helix bundle tags and the ...
11
Conformational Changes in the Hepatitis B Virus Core Protein Are Consistent with a Role for Allostery in Virus Assembly
... four-helix bundle (amino acids 49 to 109), with two helices contributed by each half ...This bundle forms the spikes that punctuate the virus surface (5, ...a helix- turn-extended structure ...
9
Anti HIV 1 activity of anionic polymers: a comparative study of candidate microbicides
... six- helix bundles which were undetectable or present at low levels in untreated virus particles ...six helix bundle formation was caused by contact of virus particles with the micronized form of CAP ...
11
The Avian Retrovirus Avian Sarcoma/Leukosis Virus Subtype A Reaches the Lipid Mixing Stage of Fusion at Neutral pH
... We previously showed that the envelope glycoprotein (EnvA) of avian sarcoma/leukosis virus subtype A (ASLV-A) binds to liposomes at neutral pH following incubation with its receptor, Tva, at > 22°C. We also provided ...
9
Functional Studies of Ferritin 3-Fold Axis: Effects of Mutations Near Subunit Interaction Sites
... It is difficult to determine the possible reason(s) for the difference in behavior of the mutants from the three dimensional structure because the tertiary and quaternary structures of H and M ferritins are identical [7, ...
46
Biochemical and biophysical characterisation of the quartromicin polyketide synthase
... 4-helix bundle with a second ACPdd 130 and a C-terminal docking helix that plays a vital role in facilitating in- teractions with a downstream KSdd (Figure ...C-terminal helix and the KSdd ...
202
Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus
... 6-helix bundle would bring the cellular and viral lipid bilayers into proximity, allowing membrane fusion ...to bundle assembling, however, HR1 and HR2 should expe- rience a transient intermediate ...
7
Crystal Structures of Beta- and Gammaretrovirus Fusion Proteins Reveal a Role for Electrostatic Stapling in Viral Entry
... Retroviruses are a distinct family of enveloped RNA viruses that are classified into seven genera: alpha-, beta-, delta-, epsilon-, and gammaretroviruses; lentivirus; and spumavirus. The retrovi- ral envelope ...
11
Mechanistic Studies of a T20-Dependent Human Immunodeficiency Virus Type 1 Variant
... We proposed several possible mechanistic models for the unique T20-dependent replication phenotype (3). First, it could be envisaged that the T20 peptide is actively involved in the formation of the 6-helix ...
6
Elucidating the Energetics of Bacterial Signal Transduction: Insights From Phoq
... For another view of the data, we consult fits of the model to raw experimental data and grouped the fits by the various locations of the mutations in the protein (Fig. 3.7). Two conclusions are immediately apparent. ...
133
Role of a Putative gp41 Dimerization Domain in Human Immunodeficiency Virus Type 1 Membrane Fusion
... Dimeric association in the structure of C39. The fusion- inhibitory peptide T-20 corresponds to the central segment of the C-terminal ectodomain of gp41 (Fig. 1). The C54 structure suggests that T-20 retains key ...
9
Role of eosinophils in respiratory syncytial virus infection
... six-helix bundle structure airway hyperresponsiveness antigen presenting cell bronchoaiveolar lavage bronchoaiveolar lavage fluid boron-dipyrromethene bovine serum albumin CCAAT-enhancer[r] ...
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