Heparan Sulphate
Effect of heparin and heparan sulphate on open promoter complex formation for a simple tandem gene model using ex situ atomic force microscopy
... Biochemical methods overcome the issue of non-specifically bound RNAPs by the addition of the molecule heparin, which com- petes with DNA to bind RNAP in the DNA binding channel [33]. Heparin is a polyanionic ...
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Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans
... for heparan sulphate (HS) GAG polysaccharides), dot blots with a HS-specific antibody showed heparan sulphate proteoglycans (HSPGs) to be present, consistent with Glossina morsitans morsitans ...
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Molecular Modelling of the Complex Polysaccharide Heparan Sulphate
... Heparan Sulphate plays an important role in many life processes and so an understanding of its role as a universal co-receptor is of great importance. Traditionally oligosaccharides derived from the related ...
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Interplay between transglutaminases and heparan sulphate in progressive renal scarring
... Transglutaminase-2 (TG2) is a new anti-fibrotic target for chronic kidney disease, for its role in altering the extracellular homeostatic balance leading to excessive build-up of matrix in kidney. However, there is no ...
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Genetic variations in genes involved in heparan sulphate biosynthesis are associated with Plasmodium falciparum parasitaemia: a familial study in Burkina Faso
... of heparan sulphate, owing to genetic varia- tions within genes encoding the enzymes ...of heparan sulphate (HS); the sulphation level is a measure of the completion of this synthesis, and is ...
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Hydrogen peroxide as a potential mediator of the transcriptional regulation of heparan sulphate biosynthesis in keratinocytes
... Therefore, the increased expression of the anti-heparan sulfate antibody epitope (10E4) in the catalase transfectants appears to be due to increased modification of the HS chain rather than increased synthesis of ...
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Apolipoprotein E lipoprotein particles inhibit amyloid-β uptake through cell surface heparan sulphate proteoglycan
... To determine whether the observed effects of apoE particles on A β uptake are also relevant in neurons, we analyzed the effects of apoE3 particles on A β uptake in the presence or absenc[r] ...
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TLR4 dependent heparan sulphate induced pancreatic inflammatory response is IRF3 mediated
... Heparin by-products from beef lung were treated with alkaline copper sulphate (to remove dermatan sulphate) after papain digestion. The calcium salt was then precipi- tated with 36% ethanol (to separate ...
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The role of heparan sulphate in the development of mouse embryoid bodies
... Abbreviations ASC Adult stem cell BM Basement membrane BMP Bone morphogenic protein CEE Columnar epiblast epithelium CRC Colorectal cancer DAPI%%%%%4',6FdiamidinoF2Fphenylindole DMEM Dul[r] ...
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Characterisation of a novel bioactive complex polysaccharide from a marine invertebrate with potent anticancer and antimalarial activities
... chondroitin sulphate (CS), dermatan sulphate (DS), keratan sulphate (KS), heparin (Hep), and perhaps the most widely studied is heparan sulphate ...their sulphate substituents ...
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Matrix expansion and syncytial aggregation of syndecan 1+ cells underpin villous atrophy in coeliac disease
... of heparan sulphate and sulphated GAGs within the subepithelial basement membrane and on the basolateral surface of epithelial cells, most marked towards the villous ...
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EFFECT OF VARIOUS INORGANIC MICRO-NUTRIENTS ON GROWTH, BIOMASS AND HYDROCARBON YIELD OF EUPHORBIA LATHYRIS L. A HYDROCARBON YIELDING PLANT
... The response of Nitroge also depend on how well the crop is supplied with other nutrients (Gartner, 1969). Without Phosphorus and potassium applications, the yield response to increasing Nitrogen levels was smaller than ...
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Isolation, Characterization of Protease Producing Microbes from Soil of Agriculture Land and Purifcation of Protease
... The lysed culture broth supernatant was concentrated by ammonium sulphate precipitation. The optimum activity was obtained at 60 % (w/v) saturation with 6 fold increase in the specific activity compared to the ...
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PubMedCentral-PMC5450942.pdf
... NRP1 is a coreceptor for plexinA expressed by neurons and plays an important role in axonal targeting and growth cone collapse induced by Semaphorin 3a (Sema3a), for example in dorsal root ganglia (DRG) neurons. 20,21 ...
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Normal levels of anticoagulant heparan sulfate are not essential for normal hemostasis
... critical enzyme was purified, cloned, and identified as the long-sought heparan sulfate 3-O-sulfotransferase-1 (3-OST-1) (19, 20), which preferentially modifies select- ed HS structures to create the above ...
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A MODIFIED AND IMPROVED METHOD FOR ISOLATION OF METAGENOMIC DNA FROM HILLY AND DENSE FOREST SOIL SAMPLES
... aluminium sulphate, aluminium ammonium sulphate, calcium carbonate and PVPP were given in conjunction with CTAB extraction buffer but humic acid was not efficiently ...
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Drosophila Pipe protein activity in the ovary and the embryonic salivary gland does not require heparan sulfate glycosaminoglycans
... a heparan sulfate ...of heparan sulfate would also affect Alcian Blue staining in the embryonic salivary ...in heparan sulfate; sulfateless (sfl) encodes a protein with similarity to vertebrate ...
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The enzymatic degradation of heparan sulfate
... Sulfamidase Sulfamidase cleaves the sulfate from the amino group of GlcNS residues of HS and has been purified from a number of human tissues and recombinant cell lines [148–150].. Sulfa[r] ...
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Heparan Sulfate Dependent Enhancement of Henipavirus Infection
... Virus infection and titration. Nipah virus (isolate UMMC1; Gen- Bank accession number AY029767) (42), recombinant NiV expressing enhanced green fluorescent protein (45), and Hendra virus (Australia/ horse/1994) obtained ...
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Molecular diversity of heparan sulfate
... Heparan sulfate (HS) appeared early in metazoan evo- lution. As such, many of the structural motifs (variably sulfated disaccharide subunits) that characterize HS (and heparin) were established early on and have ...
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