hepatitis B Polymerase protein
Protein-Primed Terminal Transferase Activity of Hepatitis B Virus Polymerase
... -independent protein-primed DNA synthesis by ...HP. Protein priming (either the initiation or polymerization stage) as templated by H ε is insensitive to PFA ...HP. Protein-primed and ...
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In Vitro Epsilon RNA-Dependent Protein Priming Activity of Human Hepatitis B Virus Polymerase
... l protein A/G beads (Pierce) prebound with the M2 anti-FLAG antibody (Sigma) (hereafter referred to as “M2 beads”) per 100-mm dish of transfected ...fluorescence protein (GFP)- transfected cells in ...
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Hepatitis B Virus Polymerase Localizes to the Mitochondria, and Its Terminal Protein Domain Contains the Mitochondrial Targeting Signal
... (STING) protein to block IFN-  responses, and the RT and RNase H domains of Pol are sufficient for this effect ...regulatory protein specific to mammalian hep- adnaviruses, has also been detected at ...
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Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase.
... a protein-primed reaction that involves the covalent linkage of the first deoxyribonucleotide to the polymerase ...human hepatitis B virus (HBV) reverse transcriptase (pol) in insect cells by ...
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Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template.
... What is the alteration? There are many changes to P that may cause the alteration that we detected as increased resis- tance to proteolysis, including covalent modification, complex formation with cellular proteins, or ...
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Human Hepatitis B Virus Polymerase Interacts with the Molecular Chaperonin Hsp60
... 60-kDa protein specifically interacts with human HBV ...84-kDa protein was immuno- stained with the M2 monoclonal antibody, which is specific to the FLAG epitope, and represents recombinant human HBV Pol ...
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Effects of Genomic Length on Translocation of Hepatitis B Virus Polymerase-Linked Oligomer
... Plasmids. HBV mutants used in this report were derived from plasmid pMH- 9/3091 subtype ayw (10). Plasmid pSHH2.1 and helper plasmids pMTP and pMT1883 were described previously (3, 4, 30). The HBV sequence was num- bered ...
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Comparative Analysis of Hepatitis B Virus Polymerase Sequences Required for Viral RNA Binding, RNA Packaging, and Protein Priming
... for protein priming are dramatically different from those for ...for protein priming in the pres- ence of either Mg 2⫹ (Hε dependent) or Mn 2⫹ (Hε ...out protein priming efficiently without the RNase ...
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Duck hepatitis B virus polymerase acts as a suppressor of core protein translation.
... Immunoprecipitation. Fifty microliters of each in vitro-translated product was incubated on ice with 150 m l of RIPA buffer (150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate [SDS], and ...
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Mapping of Functional Subdomains in the Terminal Protein Domain of Hepatitis B Virus Polymerase
... Conserved regions align with secondary structure elements in the TP domain. To identify conserved and, thus, potentially functionally important regions in the TP domain, all known HBV (n ⫽ 583) and DHBV (n ⫽ 45) ...
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Properties of Monoclonal Antibodies Directed against Hepatitis B Virus Polymerase Protein
... This report describes the generation and characterization of MAbs against the full-length HBV Pol protein and their value for study of this protein which plays a central role in the viral life cycle. A ...
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Inhibition of Hepatitis B Virus Polymerase by Entecavir
... As a means to rigorously validate our HBV molecular model, we modeled HBV RT containing LVDr substitutions L180M and M204V with ETV-TP (Fig. 6). The M204 residue is part of the YMDD loop, which anchors the triphosphate ...
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Effects of insertional and point mutations on the functions of the duck hepatitis B virus polymerase.
... To assist in the assignment of these functions to domains of the duck hepatitis B virus polymerase protein, we have constructed a series of substitution mutations and a large insertion m[r] ...
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Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymerase.
... Recently, we demonstrated that the polymerase of duck hepatitis B virus DHBV synthesized in an in vitro translation reaction exhibits reverse transcriptase activity and that the viral po[r] ...
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DNA synthesized in the hepatitis B Dane particle DNA polymerase reaction.
... To determine the size of the DNA molecules containing Dane particle DNA base sequences in HBV-infected liver, infected liver DNA was denatured with alkali and fractionated by sedimentati[r] ...
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Occult hepatitis B virus infection: influence of S protein variants
... After eliminating technical bias, we sought to eluci- date the underlying mechanisms involved in OBI pathogenesis, which have been suggested to be related to genetic variation, HBV reproduction levels, gene integration, ...
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Hepatitis B virus x protein induces epithelial mesenchymal transition of hepatocellular carcinoma cells by regulating long non coding RNA
... down-regulated or up-regulated by more than 2-fold in the HepG2 cells because of HBx. Of them, the ZEB2- AS1 (ZEB2-antisense RNA1), a known-EMT related lncRNA, was up-regulated by 3.55-fold. We first verified the ...
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Protein Kinase Activity in Hepatitis B Virus
... Only a fraction of the major polypeptide apparent size, 19,700 daltons in Dane particle cores and hepatitis B core antigen particles from infected liver appeared to be phosphorylated, an[r] ...
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The Role of HBx-mediated Transcriptional Activities and Epigenetic Alterations in Hepatitis B Virusinduced Hepatocellular Carcinoma
... HBx protein induces the hypermethylation of several tumour suppressor genes by modulating the transcriptional activation of DNMTs that result in the loss of gene expression and normal functions leading to ...
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Distinct patterns of serum hepatitis B core-related antigen during the natural history of chronic hepatitis B
... of hepatitis B virus [16], in which a new no- menclature is used to describe these four phases, ...chronic hepatitis, HBeAg-negative chronic infection and HBeAg-negative chronic ...
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