Hepatitis C virus RNA
Strengths and Limitations of Commercial Tests for Hepatitis C Virus RNA Quantification
... HCV RNA assays in the diagnosis and management of chronic hepatitis C is ...baseline hepatitis C virus (HCV) RNA level was associated with a decreased likelihood of a ...
5
Ultracentrifugation of Serum Samples Allows Detection of Hepatitis C Virus RNA in Patients with Occult Hepatitis C
... HCV RNA levels tested by real-time RT-PCR (see ...by hepatitis B virus, autoimmunity, genetic disorders, alcohol intake, drug toxicity, ...immunodeficiency virus. All patients had genomic HCV ...
6
Oligomerization and Cooperative RNA Synthesis Activity of Hepatitis C Virus RNA-Dependent RNA Polymerase
... Due to the essential role of NS5B protein in the HCV replication and infection process (22), this enzyme has been studied extensively. In addition, the crystal structure of purified HCV NS5B has been solved at high ...
8
Membrane Association of the RNA-Dependent RNA Polymerase Is Essential for Hepatitis C Virus RNA Replication
... the C-terminal domain of NS5B contains a cis-acting replication element (CRE) that is essential for HCV RNA replication ...cruciform RNA element, designated ...
7
Evaluation of the MagNA Pure LC Instrument for Extraction of Hepatitis C Virus RNA for the COBAS AMPLICOR Hepatitis C Virus Test, Version 2 0
... 2.0) hepatitis C virus (HCV) RNA extraction versus automated HCV RNA extraction by using the MP ...HCV RNA (standard 96/ 790) ...
6
Qualitative Detection of Hepatitis C Virus RNA: Comparison of Analytical Sensitivity, Clinical Performance, and Workflow of the Cobas Amplicor HCV Test Version 2 0 and the HCV RNA Transcription Mediated Amplification Qualitative Assay
... HCV RNA TMA qualitative test (HCV ...HCV RNA is captured to magnetic particles coated with oligonucleotides com- plementary to the 5⬘ untranslated region of the HCV ...control RNA to each sample. The ...
5
DDX3 DEAD-Box RNA Helicase Is Required for Hepatitis C Virus RNA Replication
... double-stranded RNA and separate the RNA strands or might contribute to the function of HCV NS3 ...replicon RNA was also partially suppressed in DDX3 knockdown cells ...fering RNA-mediated ...
5
Visualization of Double-Stranded RNA in Cells Supporting Hepatitis C Virus RNA Replication
... in hepatitis C virus (HCV) RNA replication are unknown, and this aspect of the virus life cycle is not ...that virus-encoded nonstructural proteins and RNA genomes ...
14
Template Requirements for RNA Synthesis by a Recombinant Hepatitis C Virus RNA-Dependent RNA Polymerase
... the C-terminal tail (19, 26). The C-ter- minal tail of NS5B is present in the active site of the crystal structure and has been hypothesized to play a role in template discrimination ...the C termini ...
8
Modulation of hepatitis C virus RNA Abundance.pdf
... deficiency virus tat protein and induce secretion of alkaline phosphatase (SEAP) in these cells in a manner that quantitatively reflects the intra- cellular abundance of the replicon RNAs ...affect RNA ...
6
3′ Nontranslated RNA Signals Required for Replication of Hepatitis C Virus RNA
... nontranslated RNA (3 ⴕ NTR) signals required for replication of subgenomic hepatitis C virus (HCV) ...viral RNA in transiently transfected hepatoma ...detectable RNA replication ...
12
Mutational analysis of the hepatitis C virus RNA helicase.
... Protein expression and purification. Figure 1A shows the amino acid sequences conserved among known RNA helicases. The mutated positions and the amino acids substituted are illustrated in Fig. 1B. We used four ...
10
The α Isoform of Protein Kinase CKI Is Responsible for Hepatitis C Virus NS5A Hyperphosphorylation
... by RNA interference ...HCV RNA and silencing efficiency of the kinase by quantitative PCR ...HCV RNA slowly decreased and reached a min- imum of 40% with respect to that of the mock-transfected cells ...
8
De Novo Initiation of RNA Synthesis by the RNA-Dependent RNA Polymerase (NS5B) of Hepatitis C Virus
... the virus (6, 50, ...viral RNA genome (33, 57, ...other RNA viruses, to which reverse genetics is applicable (1, 47, 64), functions of the cis signal sequences and viral proteins of HCV in the ...
13
Prediction of linear B-cell epitopes of hepatitis C virus for vaccine development
... Development of an effective vaccine against hepatitis C virus (HCV) is a complex task owing to the variability of this RNA virus. Recently, development of HCV vaccines has mainly ...
13
Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.
... plant virus brome mosaic virus (7, 15, 24, 46), the enzyme was found to be associated with intracellular membranes but could be solubilized with buffers containing high concentrations of salt and ...of ...
13
Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... of RNA templates af- fects the ...the RNA template and polymerase and (ii) interaction between NTP and the polymerase/template ...certain RNA secondary structure is required to initiate de novo ...
10
Structure of Hepatitis C Virus Polymerase in Complex with Primer-Template RNA
... an RNA template for ...polio- virus RdRp was shown to adopt different conformations depend- ing on the incoming NTP, translocation state, and presence of divalent metal ions ...primer-template RNA ...
9
Assessment of carotid intima-media thickness and carotid plaque formation among patients with ischemic stroke and hepatitis C virus infection
... rate, C-reactive protein, fasting and postprandial blood glucose, liver function tests, renal function tests, and lipid ...HCV RNA positivity, and they subdi- vided into low and high viral ...
6
Molecular Detection of HGV RNA in Chronic Hepatitis Patients from Afzalipoor General Hospital in Kerman, Iran
... this virus have been proposed based on the sequence analysis of the 5´ GBV- noncoding region 5´ (NCR) or E2 ...fulminant hepatitis due to HGV infection have been reported, but it remains unclear whether the ...
7