Herpes simplex thymidine kinase
Selective assay for herpes simplex viruses expressing thymidine kinase.
... Introduction of herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA.. Transfer of thymidine kinase to thymidine kinaseless L cells by infe[r] ...
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Development and analysis of a transformation-defective mutant of Harvey murine sarcoma tk virus and its gene product.
... Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of Herpes simplex virus type 1.. Predicted n[r] ...
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Reactivation of thymidine kinase-defective herpes simplex virus is enhanced by nucleoside.
... Herpes simplex virus (HSV) mutants defective for thymidine kinase expression (TK 2 ) have been reported to establish latent infection of sensory ganglia of mice, in that HSV latency-associated ...
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Thymidine Kinase-Negative Herpes Simplex Virus 1 Can Efficiently Establish Persistent Infection in Neural Tissues of Nude Mice
... ABSTRACT Herpes simplex virus 1 (HSV-1) establishes latency in neural tissues of immunocompetent mice but persists in both peripheral and neural tissues of lymphocyte-deficient ...mice. Thymidine ...
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Rapid Phenotypic Characterization Method for Herpes Simplex Virus and Varicella Zoster Virus Thymidine Kinases To Screen for Acyclovir Resistant Viral Infection
... for herpes simplex virus (HSV) and varicella-zoster virus (VZV) thymidine kinase (TK) genes was developed for monitoring acyclovir-resistant ...
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EBNA1 can link the enhancer element to the initiator element of the Epstein-Barr virus plasmid origin of DNA replication.
... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ...
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Isolation of cis-acting vaccinia virus DNA fragments promoting the expression of herpes simplex virus thymidine kinase by recombinant viruses.
... TK- cells were infected with one of these recombinant viruses and then transfected with pools of chimeric plasmids composed of a cloned herpes simplex virus thymidine kinase gene which c[r] ...
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Glycoprotein D of Bovine Herpesvirus 5 (BoHV-5) Confers an Extended Host Range to BoHV-1 but Does Not Contribute to Invasion of the Brain
... of thymidine kinase-negative baby hamster kidney (BHK) cells selected for their property of being resistant to infection with herpes simplex virus type 1 (HSV-1), HSV-2, and ...
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Alterations in Substrate Specificity and Physicochemical Properties of Deoxythymidine Kinase of a Drug-Resistant Herpes Simplex Virus Type 1 Mutant
... Characterization of pyrimidine deoxyribonucleoside kinase thymidine kinase and thymidylate kinase as a multifunctional enzyme in cells transformed by herpes simplex virus type 1 and in c[r] ...
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Homopolymer mutational hot spots mediate herpes simplex virus resistance to acyclovir.
... Nucleotide sequence changes in thymidine kinase gene of herpes simplex virus type 2 clones from an isolate of a patient treated with acyclovir.. Brief report: recurrent acyclovir-resista[r] ...
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Herpes simplex virus thymidine kinase and specific stages of latency in murine trigeminal ganglia.
... 6903 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest From marker rescue, sequencing, transcript, and latency analyses of the thymidine kinase-negative herpes simplex vir[r] ...
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[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.
... [125I]deoxycytidine Used in a Rapid, Sensitive, and Specific Assay for Herpes Simplex Virus Type 1 Thymidine Kinase WILLIAM C.. SUMMERS Departments of Therapeutic Radiology,* Molecular B[r] ...
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Association between sensitivity of viral thymidine kinase associated acyclovir resistant herpes simplex virus type 1 and virulence
... the thymidine kinase (TK) genes, and the remaining having mu- tations in the viral DNA polymerase genes ...Viral thymidine kinase-deficient HSV-1 impaired viral replication, viru- lence, ...
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Mutation Spectra of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants
... the thymidine kinase (TK) mutagenesis assay was applied to iso- late drug-resistant tk mutants from both the wild-type strain KOS and the Y7 recombinant (6, 7, 9), and mutants were subjected to detailed ...
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Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene.
... GALLOWAY* Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 We have determined the complete nucleotide sequence of the thymidine kinase gene of herpes simplex virus HSV t[r] ...
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Article #780
... A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma Ariane Söling*1,[r] ...
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Rapid typing of herpes simplex virus based on immunological specificity of viral thymidine kinase and typing according to sensitivity to iododeoxyuridine
... Rapid Typing of Herpes Simplex Virus Based on Immunological Specificity of Viral Thymidine Kinase and Typing According to Sensitivity to lododeoxyuridine.. We describe two methods for ty[r] ...
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Introduction of Three Independent Selection Markers in Leishmania
... markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated ...
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Human Thymidine Kinase Can Functionally Replace Herpes Simplex Virus Type 1 Thymidine Kinase for Viral Replication in Mouse Sensory Ganglia and Reactivation from Latency upon Explant
... several kinase activities, including those found separately in hTK, hdCK, and ...which kinase activity is important for replication and reactiva- tion in mouse ganglia, viruses expressing these individual ...
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Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpesvirus gene homologs.
... Nucleotide sequence of the herpes simplex virus type 2 HSV-2 thymidine kinase gene and predicted amino acid sequence of the thymidine kinase polypeptide and its comparison with the HSV-1[r] ...
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