herpes simplex virus-thymidine kinase
Isolation of cis-acting vaccinia virus DNA fragments promoting the expression of herpes simplex virus thymidine kinase by recombinant viruses.
... TK- cells were infected with one of these recombinant viruses and then transfected with pools of chimeric plasmids composed of a cloned herpes simplex virus thymidine kinase gene which c[r] ...
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A conserved open reading frame that overlaps the herpes simplex virus thymidine kinase gene is important for viral growth in cell culture.
... Nucleotide sequence of the herpes simplex virus type 2 HSV-2 thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide and its comparison with the HSV-1 thy[r] ...
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Positive control of the herpes simplex virus thymidine kinase gene requires upstream DNA sequences.
... HALPERN Pathology Department, McMaster University, Hamilton, Ontario, Canada L8N 3Z5 We examined the ability of deleted versions of the herpes simplex virus type 1 thymidine kinase gene,[r] ...
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Selective assay for herpes simplex viruses expressing thymidine kinase.
... Introduction of herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA.. Transfer of thymidine kinase to thymidine kinaseless L cells by infe[r] ...
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Thymidine plaque autoradiography of thymidine kinase positive and thymidine kinase negative herpesviruses
... Herpes simplex virus thymidine kinase expression in trigeminal ganglion infection: correlation of enzyme activity with ganglion virus titer and evidence of in vivo complementation.. Alte[r] ...
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Introduction of Three Independent Selection Markers in Leishmania
... markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ ...
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Genetic and biochemical characterization of the thymidine kinase gene from herpesvirus of turkeys.
... A conserved open reading frame that overlaps the herpes simplex virus thymidine kinase gene is important for viral growth in cell culture.. Induction of both thymidine and deoxycytidine [r] ...
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Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate
... Background: Cancer gene therapy by retroviral vectors is mainly limited by the level of transduction. Retroviral gene transfer requires target cell division. Cell synchronization, obtained by drugs inducing a reversible ...
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The effects of insertions on mammalian intrachromosomal recombination.
... A series of mouse L cell lines carrying mutant herpes simplex virus thymidine kinase (tk) heteroalleles was generated; these lines differed in the nature of their ins[r] ...
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Reactivation of thymidine kinase-defective herpes simplex virus is enhanced by nucleoside.
... Herpes simplex virus (HSV) mutants defective for thymidine kinase expression (TK 2 ) have been reported to establish latent infection of sensory ganglia of mice, in that HSV ...
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Thymidine Kinase-Negative Herpes Simplex Virus 1 Can Efficiently Establish Persistent Infection in Neural Tissues of Nude Mice
... susceptible to T-cell scrutiny in mouse neural tissues remains to be elucidated. In the brain stem of ICR nude mice infected with tkLTRZ1, we found less virus on days 3 and 7 p.i. than on day 28 p.i., by 58- and ...
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EBNA1 can link the enhancer element to the initiator element of the Epstein-Barr virus plasmid origin of DNA replication.
... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ...
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Association between sensitivity of viral thymidine kinase associated acyclovir resistant herpes simplex virus type 1 and virulence
... of virus inoculation, virus titers in tears of mice inoculated with each of HSV-1 clones through corneal inoculation were mea- sured ...The virus load kinetics in tears of HSV-1 CL18 and CL19 were ...
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[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.
... [125I]deoxycytidine Used in a Rapid, Sensitive, and Specific Assay for Herpes Simplex Virus Type 1 Thymidine Kinase WILLIAM C.. SUMMERS Departments of Therapeutic Radiology,* Molecular B[r] ...
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Alterations in Substrate Specificity and Physicochemical Properties of Deoxythymidine Kinase of a Drug-Resistant Herpes Simplex Virus Type 1 Mutant
... Characterization of pyrimidine deoxyribonucleoside kinase thymidine kinase and thymidylate kinase as a multifunctional enzyme in cells transformed by herpes simplex virus type 1 and in c[r] ...
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Article #780
... A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma Ariane Söling*1,[r] ...
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Nucleotide sequence of the herpes simplex virus type 2 thymidine kinase gene.
... GALLOWAY* Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 We have determined the complete nucleotide sequence of the thymidine kinase gene of herpes simplex virus HSV t[r] ...
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Mutation Spectra of Herpes Simplex Virus Type 1 Thymidine Kinase Mutants
... the thymidine kinase (TK) mutagenesis assay was applied to iso- late drug-resistant tk mutants from both the wild-type strain KOS and the Y7 recombinant (6, 7, 9), and mutants were subjected to detailed ...
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Herpes Simplex Virus Type 1 Thymidine Kinase Sequence Fused to the lacZ Gene Increases Levels of β-Galactosidase Activity per Genome of High-Capacity but Not First-Generation Adenoviral Vectors In Vitro and In Vivo
... gene expression per vg would allow further reduction of the viral vector doses needed to achieve a therapeutic efficacy. WPRE has been systematically evaluated as a posttranscrip- tional regulatory element to increase ...
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Rapid typing of herpes simplex virus based on immunological specificity of viral thymidine kinase and typing according to sensitivity to iododeoxyuridine
... Rapid Typing of Herpes Simplex Virus Based on Immunological Specificity of Viral Thymidine Kinase and Typing According to Sensitivity to lododeoxyuridine.. We describe two methods for ty[r] ...
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