heteroduplex DNA
Efficient Incorporation of Large (>2 kb) Heterologies Into Heteroduplex DNA: Pms1/Msh2-Dependent and -Independent Large Loop Mismatch Repair in Saccharomyces cerevisiae
... DNA double-strand break (DSB) repair in yeast is effected primarily by gene conversion. Conversion can conceivably result from gap repair or from mismatch repair of heteroduplex DNA (hDNA) in ...
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Cleavage of Mispaired Heteroduplex DNA Substrates by Numerous Restriction Enzymes
... whether heteroduplex DNA cleavage required or was stimulated by high glycerol ...6V/+ DNA did not require high glycerol concentration but glycerol appeared to stimulate heteroduplex DNA ...
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Heteroduplex DNA formation is associated with replication and recombination in poxvirus-infected cells.
... MCFADDEN, 1988 High levels of genetic recombination among cotransfected plasmid DNAs in poxvirus-infected mammalian cells. KOLODNER, 1988 Effect of DNA struc-[r] ...
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Incorporation of Large Heterologies Into Heteroduplex DNA During Double-Strand-Break Repair in Mouse Cells
... In this study, the formation and repair of large (⬎1 kb) insertion/deletion (I/D) heterologies during double-strand-break repair (DSBR) was investigated using a gene-targeting assay that permits efficient recovery of ...
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Heteroduplex DNA formation and homolog pairing in yeast meiotic mutants.
... As suggested above for hopl, the higher pairing values observed by WEINER and KLECK- NER (1994) might be due to their ability to monitor chromosome pairing at all [r] ...
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Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination.
... All frequencies were different from wild-type at the P 5 0.05 level, except those frequencies indicated by an asterisk (*). Refers to the fold change of the recombination frequ[r] ...
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Poorly Repaired Mismatches in Heteroduplex DNA are Hyper-Recombinagenic in Saccharomyces cerevisiae
... A model is proposed in which the high PMS allele blocks an other- wise long mismatch repair tract initiated by the normal allele, and thus causes hyperrecombination b[r] ...
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Chi enhances heteroduplex DNA levels during recombination.
... hDNA formation, we measured hDNA in replication- blocked lambda crosses performed in these and other E. T h e results of these crosses, as well as the enzymatic activities an[r] ...
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Heteroduplex DNA and meiotic recombination in Drosophila
... Most non-PMS NCOs from mei-9 mutants are indistinguishable from NCOs from wild-type: An essential requirement for MEI-9 in MMR predicts that all NCOs from mei-9 mutants will exhi[r] ...
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Repair of heteroduplex DNA in Xenopus laevis oocytes.
... As part of our characterization of the mechanism of homologous recombination in Xenopus oocytes, we un- dertook a study of the distribution of multiple heteroal- lelic markers in [r] ...
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Heteroduplex Mapping of Heat-Resistant Deletion Mutants of Bacteriophage T5
... Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.[r] ...
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The Conversion Gradient at HIS4 of Saccharomyces cerevisiae. I. Heteroduplex Rejection and Restoration of Mendelian Segregation
... for DNA double-strand breaks ...upon heteroduplex DNA—aberrant segregation frequencies at the low end of the gradient are higher in the absence of mismatch ...The heteroduplex rejection model ...
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Mismatch repair-induced meiotic recombination requires the pms1 gene product.
... The pmsl-1 mutation reduces the frequency of mismatch repair-induced events: We wished to test the hypothesis that the repair of heteroduplex DNA containing closely [r] ...
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Mechanisms Involved in Targeted Gene Replacement in Mammalian Cells
... (i) heteroduplex DNA (hDNA) is formed during mammalian gene replacement; (ii) mismatches in hDNA are usually efficiently repaired before DNA replication and cell division; (iii) the gene replacement ...
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The Effect of Mismatch Repair and Heteroduplex Formation on Sexual Isolation in Bacillus
... Comparison of Bacillus and other recombination sys- tems: Although the exponential relationship between sexual isolation and sequence divergence has been ob- served in several systems, it is clear that the underlying ...
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Rapid Detection of HIV-1 Subtypes in Ghana by Heteroduplex Mobility Assay
... Using the HMA technique, fifteen these newly isolated Ghanaian HIV-1 strains were subtyped. Having thus confirmed, prevalent Ghanaian HIV strains could be subtyped directly by HMA following proviral DNA extraction ...
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Simplified Hepatitis C Virus Genotyping by Heteroduplex Mobility Analysis
... (27). Heteroduplex mobility analysis (HMA) relies on the forma- tion of mismatches when two divergent DNA molecules are mixed, denatured, and allowed to ...
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Highly Mismatched Molecules Resembling Recombination Intermediates Efficiently Transform Mismatch Repair Proficient Escherichia coli
... Heteroduplex molecules with multiple mismatches ef- ficiently transform Mutf and Mut- strains: To assess the ability of MMR to act on a multiply mismatched recombi- nat[r] ...
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Heteroduplex analysis of the RNA of clone 3 Moloney murine sarcoma virus.
... DUESBERG,3 AND NORMAN DAVIDSON'* Received for publication 25 June 1979 Heteroduplex analysis of the RNA isolated from purified virions of clone 3 Moloney murine sarcoma virus M-MSV hybri[r] ...
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Distribution of Chi-stimulated recombinational exchanges and heteroduplex endpoints in phage lambda.
... (All phage titers are p.f.u. plex-containing recombinants from these crosses are shown in Table 6. Recombinants in intervals I , 11, IIIa and IIIb are scored as very cl[r] ...
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