high resolution melt
High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
... High resolution melt (HRM) analysis is a technique that measures the disassociation of double-stranded DNA at high temperature resolution, and permits the analysis of genetic variations ...
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Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
... 2. Briefly, targeted sequences for each marker were aligned using CLUSTAL W, and primers were designed to provide discriminating HRM profiles. The marker specificities were as follows: Bspp, all Brucella spp.; Bmel, B. ...
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Integrated Microfluidic Card with TaqMan Probes and High Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genes
... ABSTRACT Genotypic methods for drug susceptibility testing of Mycobacterium tuberculosis are desirable to speed the diagno- sis and proper therapy of tuberculosis (TB). However, the numbers of genes and polymorphisms ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... a high-resolution melt (HRM) PCR assay for the differ- entiation of the four serovars of ...distinct melt profiles that distinguish clonal representatives of serovars 1, 3, 6, and ...
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A refined, rapid and reproducible high resolution melt (HRM) based method suitable for quantification of global LINE 1 repetitive element methylation
... using high resolution melt (HRM) curve analysis technology - a quantitative methylation- sensitive real-time fluorescence-based PCR method ...of high quality ...a high-throughput and ...
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A PCR–High Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
... the high rates of misidentification of NTHi as the closely related commensal ...PCR– high-resolution melt (PCR-HRM) assay for high- throughput identification of NTHi and discrimination ...
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... Of these SNPs, the C-to-T transition at position ⫺ 215 up- stream of the GTG start codon in the promoter region of the narGHJI genes was hypothesized to be responsible for the differing nitrate reductase activities ...
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Rapid High Resolution Melt Analysis of Cytauxzoon felis Cytochrome b To Aid in the Prognosis of Cytauxzoonosis
... High-resolution melt curves and differential fluorescence curves of 69 samples are shown for one of three replicate PCRs for each of the five nucleotide posi- tions ...on melt curves indicate ...
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Direct Detection of Rifampin and Isoniazid Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
... their high sensitivity is marred by their complicated procedural setup and open hybridization ...tries. High-resolution melt curve analysis (HRM) provides quantitative analysis of the ...
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High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
... efficient, high-throughput and cost effective genotyping method to track the mutations through numerous ...generations. High resolution melt (HRM) analysis has been used in a number of systems ...
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Developing a real time PCR assay based on multiplex high resolution melt curve analysis: a pilot study in detection and discrimination of soil transmitted helminth and schistosome species
... For Peer Review Developing a real time PCR assay based on multiplex high resolution melt curve analysis A pilot study in detection and discrimination of soil transmitted helminth and schistosome speci[.] ...
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Speciation of common Gram negative pathogens using a highly multiplexed high resolution melt curve assay
... High resolution melt (HRM) analysis is an end-point qPCR detection method that differentiates amplicons based on their melt profile, enables greater multiplexing capability than probe based ...
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Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis by Use of Real Time PCR and High Resolution Melt Analysis
... (7). High-resolution melt (HRM) analysis is a molecular tech- nique that can be used for detecting subtle genetic changes, such as SNPs conferring drug resistance in ...
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Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... Human infection with Chlamydophila (Chlamydia) psittaci can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications. C. psittaci is currently grouped into seven avian ...
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Differentiation of Solenopsis invicta social forms using high resolution melt PCR.
... a high resolution melt technique following preamplification of a significantly shorter Gp-9 fragment than that required for restriction endonuclease ...for high-throughput ...
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Rapid Detection and Identification of Mucormycetes in Bronchoalveolar Lavage Samples from Immunocompromised Patients with Pulmonary Infiltrates by Use of High Resolution Melt Analysis
... by high- resolution melt analysis (PCR/HRMA) to detect Rhizopus ...its high negative predictive value (99%), represents a fast and reliable tool for routine BAL sample screening for the ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epide- miological studies. The subspecies of Mycobacterium avium have different attributes but ...
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Real Time PCR and High Resolution Melt Analysis for Rapid Detection of Mycobacterium leprae Drug Resistance Mutations and Strain Types
... Drug resistance surveillance and strain typing of M. leprae are useful molecular tools for leprosy control. Traditional PCR and sequencing techniques for these applications are laborious and expensive. In order to ...
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High Resolution Melt analysis for mutation screening in PKD1 and PKD2
... HRM analysis is a high throughput technique for sequence variants screening PKD1 and PKD2 . The for- mat of PCR analysis in 96 or 394 well plates allows the process to be automated after DNA extraction and the ...
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Detection of Plasmodium Species by High Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda
... Effective PCR testing for Plasmodium species identification depends in part on the quality of the DNA obtained from blood samples. Whole blood and red blood cell pellets are reliable sources of high-quality DNA, ...
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