High resolution melt curve
Direct Detection of Rifampin and Isoniazid Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
... their high sensitivity is marred by their complicated procedural setup and open hybridization ...tries. High-resolution melt curve analysis (HRM) provides quantitative analysis of the ...
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Simultaneous Detection of Colletotrichum acutatum and C gloeosporioides from Quiescently Infected Strawberry Foliage by Real Time PCR Based on High Resolution Melt Curve Analysis
... utilizing high resolution melt curve analy- sis; 2) validate the real-time PCR assays using DNA from pure cultures of a col- lection of Colletotrichum ...
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... Of these SNPs, the C-to-T transition at position ⫺ 215 up- stream of the GTG start codon in the promoter region of the narGHJI genes was hypothesized to be responsible for the differing nitrate reductase activities ...
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Developing a real time PCR assay based on multiplex high resolution melt curve analysis: a pilot study in detection and discrimination of soil transmitted helminth and schistosome species
... For Peer Review Developing a real time PCR assay based on multiplex high resolution melt curve analysis A pilot study in detection and discrimination of soil transmitted helminth and schistosome speci[.] ...
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Speciation of common Gram negative pathogens using a highly multiplexed high resolution melt curve assay
... This high specificity is important as decisions based on the identification of the causative species have the potential to alter patient management, and false positive results could lead to the selection of ...
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A refined, rapid and reproducible high resolution melt (HRM) based method suitable for quantification of global LINE 1 repetitive element methylation
... High Resolution Melt analysis PCR (HRM PCR) is gaining acceptance as a robust method for measuring levels of methylation ...melting curve of BSC DNA to quantify methylation ...a high ...
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Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis by Use of Real Time PCR and High Resolution Melt Analysis
... of IS6110 and the RRDR of rpoB from MTC. The IS6110 marker was analyzed using melt curve analysis (MCA) of the PCR amplicons to confirm that samples were MTC strains. MCA profiles for MTC strains contained ...
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Integrated Microfluidic Card with TaqMan Probes and High Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genes
... for melt curve and difference curve analysis (C and D, G and ...aligned melt curves show melt temperature shifts versus those for the ...lower melt temperature and rightward ...
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High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
... effective, high- throughput genotyping technique is ...and melt curve analysis can all be performed in 96-well ...enables high-throughput genotyp- ing of multiple alleles of a number of genes ...
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High Resolution Melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex crop genomes
... PCR plates containing HRM amplicons were placed into a Lightscanner™ (Idaho Technology, Salt Lake City, USA) and the temperature raised to 60°C for 5 mins to ensure all samples were equilibrated. The melt tempera- ...
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Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... HRM analysis for GTpc produces distinct melt curve pro- files for all C. psittaci genotypes except genotypes D and F, which are separated using the F genotype-specific marker, GT-F (Fig. 1c). Separation ...
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Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
... utilizes high-resolution melt (HRM) analysis to specifically detect and discriminate members of this ...superb resolution and characterization of amplified nucleic acid targets by using a ...
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Rapid High Resolution Melt Analysis of Cytauxzoon felis Cytochrome b To Aid in the Prognosis of Cytauxzoonosis
... A total of 30 unique C. felis cytb genotypes have been identified; 35 nucleotide positions were determined to have single-nucleotide polymor- phisms (SNPs) (22) (Table 1). It was predicted that cytb1 could be distin- ...
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High Throughput Genotyping of Single Nucleotide Polymorphisms in the Plasmodium falciparum dhfr Gene by Asymmetric PCR and Melt Curve Analysis
... However, compared to those for the published TaqMan- based real-time PCR assay by Alker et al. (2, 3), our assay demonstrated greater sensitivity and specificity. Although the reported sensitivity of the TaqMan assay is ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... a high-resolution melt (HRM) PCR assay for the differ- entiation of the four serovars of ...distinct melt profiles that distinguish clonal representatives of serovars 1, 3, 6, and ...
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A PCR–High Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
... highest melt curve for the NTHi variants (74.6°C) and the lowest melt curve for ...relatively high annealing tem- perature used for this ...
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Differentiation of Solenopsis invicta social forms using high resolution melt PCR.
... dF/dT curve, the amplicons from homozygous (BB) samples had a single melting temperature peak at approximately 75 ± ...normalised melt curve located in between the two distinct curve types ...
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Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... in melt peaks with characteristic melting temperatures (T M ...characteristic melt curves pro- duced by the five different mosquito species after plotting a negative first derivative of fluorescence against ...
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Detection of Plasmodium Species by High Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda
... The samples utilized in this study came from a field survey of asymptomatic participants, and our results compare favorably to prior PCR studies using blood slides. Our detection rate was associated with parasite ...
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Multiplex Real Time PCR Melting Curve Assay To Detect Drug Resistant Mutations of Mycobacterium tuberculosis
... melting curve analysis have been applied to FRET probes to detect drug-resistant mutations in ...melting curve assays have been de- veloped only for the LightCycler instrument (Roche), which is a large ...
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