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Development and Validation of a Highly Accurate Quantitative Real Time PCR Assay for Diagnosis of Bacterial Vaginosis

Development and Validation of a Highly Accurate Quantitative Real Time PCR Assay for Diagnosis of Bacterial Vaginosis

In this report, we discuss the development of a highly accurate qPCR assay for BV diagnosis. This study verifies and expands upon a number of prior studies evaluating PCR-based assays for BV diagnosis. Fredricks et al. (17) assayed 17 different organisms by conventional PCR and evaluated their sensitivity and specific- ity in comparison to Amsel’s criteria and Nugent score. The high- est accuracy was reported for detection of BVAB2 or Megasphasera phylotype 1, with sensitivity and specificity of 99% and 89%, re- spectively, compared to Amsel’s criteria and 96% and 99%, re- spectively, compared to Nugent scoring. A subsequent study using qPCR found that the presence of ⬎10 9 G. vaginalis or ⬎10 8 A. vaginae genomic copies in patient specimens was 100% sensitive and 93% specific for BV diagnosis (18). Although both of these assays are highly accurate, it is important to note that they only evaluated specimens from subjects with Nugent scores of ⱕ3 or ⱖ 7; patients with intermediate flora (Nugent score of 4 to 6) were not included in either analysis. However, clinical BV by Am- sel’s criteria can be present in individuals with intermediate Nu- gent scores as well as in those with abnormal flora. Prior studies FIG 2 ROC analysis of qPCR data for combined BV-associated organisms and
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On a highly accurate approximation of the first and pure second derivatives of the Laplace equation in a rectangular parallelpiped

On a highly accurate approximation of the first and pure second derivatives of the Laplace equation in a rectangular parallelpiped

A highly accurate method is one of the powerful tools reducing the number of unknowns, which is the main problem in the numerical solution of differential equations, to get rea- sonable results. This becomes more valuable in D problems when we are looking for the derivatives of the unknown solution by the finite difference or finite element methods for a small discretization parameter h.

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The highly accurate anteriolateral portal for injecting the knee

The highly accurate anteriolateral portal for injecting the knee

Because of the associated cruciate ligaments and reflections of the synovial membrane, the intercondylar notch presents a narrow anatomic target where the synovial space and the araeolar synovial tissue cannot be easily distinguished while advancing the needle tip, mak- ing true anatomic injection difficult, resulting in only a 71% to 75% accuracy rate [2]. We hypothesized that a modified anteriolateral portal where the synovial mem- brane overlying the medial femoral condyle is the needle target (Figure 3) would be accurate and provide equiva- lent clinical outcomes as the standard lateral midpatellar approach (Figure 4). The modified anteriomedial bent knee portal proved highly accurate (97% intraarticular accuracy) when the needle tip could be felt engaging the medial femoral condyle, which compares favorably with the 93% accuracy with the lateral midpatellar approach [2]. Moreover, the modified bent knee anteriolateral portal provided equivalent clinical outcomes to the stan- dard lateral midpatellar approach (Table 1 and 2).
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Arapan S: a fast and highly accurate whole genome assembly software for viruses and small genomes

Arapan S: a fast and highly accurate whole genome assembly software for viruses and small genomes

Because of the diversity of genomes, creating a general assembler that is able to solve all cases will not be as ef- fective and fast as a specific assembler that focuses on solving particular cases. For instance, ploidy can be a serious problem when dealing with plant genomes in which tetraploidy is common. Concerning very small genomes, we believe that we can improve the accuracy of assembly of such genomes by creating an assembler that is devoted to solving small genomes. That is the reason we aimed to create an assembler (named Arapan- S) dedicated to solving small genomes. As a result, the Arapan-S assembler was able to reconstruct one very highly accurate supercontig in most cases. To check the accuracy of Arapan-S, we performed a BLAST sequence similarity search against the EBI (European Bioinformat- ics Institute) database, which includes the complete gen- omes of our dataset. This analysis showed that the Arapan-S assemblies were more than 99% accurate. We also compared Arapan-S with other well known assem- blers in the assembly of viral genomes.
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A SURVEY ON HIGHLY ACCURATE PREDICTION ALGORITHM FOR UNKNOWN WEB SERVICES WITH FUZZY CLUSTERING

A SURVEY ON HIGHLY ACCURATE PREDICTION ALGORITHM FOR UNKNOWN WEB SERVICES WITH FUZZY CLUSTERING

In HAPA (Highly Accurate Prediction Algorithm), the advantages of similarity computation and fuzzy clustering are combined, and hence increase the accuracy of prediction. Here, it computes the Pearson Correlation Coefficient (PCC) between users. For active users, who have had some web services invocation knowledge, it uses fuzzy clustering algorithm to predict the performance of web services they are going to access.

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Highly Accurate Antibody Assays for Early and Rapid Detection of Tuberculosis in African and Asian Elephants

Highly Accurate Antibody Assays for Early and Rapid Detection of Tuberculosis in African and Asian Elephants

Most elephants with active TB display no clinical signs, mak- ing it even more difficult to suspect disease (22, 25). In fact, only 15% of the culture-positive cases reported in this study had TB-suggestive symptoms prior to the time of diagnosis. Therefore, rapid and more efficient detection of infected ani- mals is crucial to improve control of TB in elephants and other zoo species, as early diagnosis allows timely initiation of chemotherapy and quarantine to prevent transmission. Us- ing sera serially collected over multiple years from 236 cap- tive elephants, we evaluated the diagnostic potentials of three serologic techniques designed for early and accurate identification of elephants infected with M. tuberculosis or M. bovis. The ElephantTB Stat-Pak, MAPIA, and the DPP VetTB test correctly identified all infected animals and pro- duced no false-negative reactions, thus demonstrating a per- fect negative predictive value and 100% sensitivity. Impor- tantly, the new serologic assays appeared to provide antemortem testing tools superior to the existing methods. Many infected elephants showed specific seroconversion years before shedding was detectable by culture of trunk FIG. 6. MAPIA testing of DBS samples. The images represent MAPIA strips developed with serum (A), plasma (B), or whole-blood (C) samples eluted from DBS after 1 week (1) or 5 weeks (2) of storage, in comparison with the standard positive and negative sera used for making DBS (0). The printed antigens are listed on the right.
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3D Printing of Intracranial Aneurysms Using Fused Deposition Modeling Offers Highly Accurate Replications

3D Printing of Intracranial Aneurysms Using Fused Deposition Modeling Offers Highly Accurate Replications

Several critical parts of neurointerventional procedures de- pend heavily on the elasticity of the anatomic structures, such as the dislocation of catheters or coils, rupture of the aneurysm, and movement of vessels in the access path. Accurate replication of these properties will greatly increase the degree of realism. Elastic building materials are available for different additive manufactur- ing techniques including FDM, and their properties should be assessed. A further limitation of our study is its retrospective de- sign with manual selection of different aneurysm morphologies. However, this approach allowed us to assess the feasibility of the technique by using a relatively small sample.
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The SMC′ Is a Highly Accurate Approximation to the Ancestral Recombination Graph

The SMC′ Is a Highly Accurate Approximation to the Ancestral Recombination Graph

a recombination event occurring in the interval ð x ; x þ dx Þ given the coalescence time s at x is s   dx : This fact, together with the fact that the joint distribution of coalescence [r]

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Endoscopic ultrasound is highly accurate and directs management in patients with neuroendocrine tumors of the pancreas

Endoscopic ultrasound is highly accurate and directs management in patients with neuroendocrine tumors of the pancreas

RESULTS: Eighty-two patients underwent 91 examinations (cases). Thirty patients had multiple endocrine neoplasia syndrome type I. One hundred pancreatic tumors were vi- sualized by EUS in 54 different patients. The remaining 28 patients had no pancreatic tumor or an extrapancreatic tu- mor. Surgical/pathological confirmation was obtained in 75 patients. The mean tumor diameter was 1.51 cm and 71% of the tumors were ⱕ 2.0 cm in diameter. Of the 54 explora- tions with surgical confirmation of a pancreatic tumor, EUS correctly localized the tumor in 50 patients (93%). Twenty- nine insulinomas, 18 gastrinomas, as well as one glu- cagonoma, one carcinoid tumor, and one somatostatinoma were localized. The most common site for tumor localiza- tion was the pancreatic head (46 patients). Most tumors were hypoechoic, homogenous, and had distinct margins. EUS of the pancreas was correctly negative in 20 of 21 patients (specificity, 95%). EUS was more accurate than angiography with or without stimulation testing (secretin for gastrinoma, calcium for insulinoma), transcutaneous ultra- sound, and CT in those patients undergoing further imaging procedures. EUS was not reliable in localizing extrapancre- atic tumors.
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Towards Highly Accurate and Stable Face Alignment for High-Resolution Videos

Towards Highly Accurate and Stable Face Alignment for High-Resolution Videos

To this end, we propose an accurate and stable algorithm for high-resolution video-based face alignment, named Frac- tional Heatmap Regression (FHR). It is well known that heatmap regression have shown its effectiveness in landmark estimation tasks (Chen et al. 2017; Newell, Yang, and Deng 2016; Chen* et al. 2018). However, Conventional Heatmap Regression (CHR) is not accurate nor stable when dealing with high-resolution facial images, since it finds the max- imum activated location in heatmaps which are generated from rounding coordinates, and thus leads to quantization errors as the heatmap resolution is much lower than the input image resolution (e.g., 128 vs. 930 shown in Fig. 1) due to the scaling operation. To address this problem, we propose a novel transformation between heatmaps and coordinates, which not only preserves the fractional part when generating heatmaps from the coordinates, but also accurately estimate the fractional part according to the 2D Gaussian function by sampling three points in heatmaps.
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Continuous glucose monitors prove highly accurate in critically ill children

Continuous glucose monitors prove highly accurate in critically ill children

Abbreviations BG: blood glucose; CGM: continuous glucose monitor; CRRT: continuous renal replacement therapy; ECMO: extracorporeal membrane oxygenation; FDA: Food and Drug Administration[r]

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Real time endobronchial ultrasound guided transbronchial needle aspiration of intrathoracic lymphadenopathy: an initial experience

Real time endobronchial ultrasound guided transbronchial needle aspiration of intrathoracic lymphadenopathy: an initial experience

effectiveness and safety of endobronchial ultrasound- transbronchial needle aspiration, the authors reported that the procedure is a safe and highly accurate for the exam[r]

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Single exponential approximation of Fourier transforms

Single exponential approximation of Fourier transforms

4.4 Exponentially Convergent Quadrature Methods For Fourier Transforms In this section we present a method for the approximation of Fourier integrals which is highly accurate and applica[r]

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Study of Dielectric Constant of (1 x) Zn xMg TiO3(ZMT) Ceramic Material at MicrowaveFrequencies as a Function of Composition x andProcessing Temperature

Study of Dielectric Constant of (1 x) Zn xMg TiO3(ZMT) Ceramic Material at MicrowaveFrequencies as a Function of Composition x andProcessing Temperature

In this paper cavity perturbation technique, which is highly accurate and advantageous in the determination of small loss tangents is used to measure the dielectric con- stant and the loss tangent of ZMT material in powder form in S-band of microwave frequencies. The accuracy of the present technique is determined through measure- ment on Teflon and comparison of results with that available in the literature [5]. The variations in dielectric properties of the ZMT material with the x-value, the cal- cination temperature and the V 2 O 5 doping for x = 0.1 are

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Accuracy of the DriveABLE cognitive assessment to determine cognitive fitness to drive

Accuracy of the DriveABLE cognitive assessment to determine cognitive fitness to drive

• This study demonstrated that the DriveABLE In-Office assessment was highly accurate in identifying drivers with suspected or confirmed cognitive impairment who would pass or fail [r]

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“Rapid HPLC-UV Method for Quantification of Valsartan in Plasma and Intestinal Perfusate for Pharmacokinetic Studies” by Muznah AlKhani, Antoun Al-Laham, Mohammed Amer Al-Mardini, Syria.

“Rapid HPLC-UV Method for Quantification of Valsartan in Plasma and Intestinal Perfusate for Pharmacokinetic Studies” by Muznah AlKhani, Antoun Al-Laham, Mohammed Amer Al-Mardini, Syria.

A simultaneous HPLC method using UV detector was developed and validated for quantification of valsartan in plasma and intestinal perfusate samples of rats. Plasma samples were purified with a simple protein precipitation procedures. An isocratic separation of valsartan was performed on reversed-phase C18 (4.6-mm × 250-mm) column. Acetonitrile-water (adjusted to pH 3.3 with formic acid)-methanol (50:40:10, v/v) was the mobile phase used at column temperature of 37°C, flow rate of 0.9 ml/min and under pump pressure of 78 bar. Wave length of detection was 230 nm. Linear responses of R 2 >0.999 in the concentration range of 100-10,000 ng/ml were observed with a short time for separation of less than 10 minutes. The method was highly accurate and precise, and the recoveries were more than 95%. Same results were seen upon analysis of samples spiked before extraction, which indicates the suitability of the developed method for application in pharmacokinetic studies.
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Efficient FPGA Implementation of a Configurable Channel Estimation Algorithm for Wireless Communication Systems

Efficient FPGA Implementation of a Configurable Channel Estimation Algorithm for Wireless Communication Systems

ABSTRACT: Wireless communication is intruding in every application realm. Cost effective solutions to design, development and testing of wireless systems is getting great interest among researchers. The efficient implementation of their components in terms of hardware cost and design time is of great importance. The major bottleneck in achieving higher throughput, data rate and lower BER in wireless communication is fading. MIMO uses multiple transmit antennas and receive antennas to improve both reliability and throughput of a receiver. Multiple antenna system requires the knowledge of channel state information (CSI) at the receiver for data recovery.Therefore highly accurate and fast estimation of wireless channel is of vital importance for error free data recovery.
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Automated Multi Meter Method for the Scaling of Low AC Voltage from 200 mV to 2 mV

Automated Multi Meter Method for the Scaling of Low AC Voltage from 200 mV to 2 mV

The first step of the scaling procedures is the calibra- tion of Fluke 8508 A DMM at a 200 mV ac voltage range using the 300 mV thin-film MJTC. This automated calibration system consists of a highly accurate program- mable calibrator (Fluke 5720 A) to precisely source both alternating and direct voltages, highly sensitive, 8.5 dig- its, DMM (Fluke 8508 A), 8.5 digits, DMM (HP 3458 A) to measure the μPot output emfs and 300 mV thin film MJTC.

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Salivary Diagnostics - Reloaded

Salivary Diagnostics - Reloaded

The challenge to make salivary diagnostics a clinical reality is in establishing the scientific foundation and clinical validations necessary to position it as a highly accurate and feasible technology, which can achieve definite point- of-care assessment of patient health and disease status.The field of salivary diagnostics is now becoming a broad, complex and crosscutting area of scientific research with enormous potential to impact the practicing dentist and health care in general.

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CT Angiography for the Detection of Cerebral Vasospasm in Patients with Acute Subarachnoid Hemorrhage

CT Angiography for the Detection of Cerebral Vasospasm in Patients with Acute Subarachnoid Hemorrhage

Vasospasm is one of the leading causes of morbid- ity and mortality in patients who survive subarach- noid hemorrhage (SAH) (1). As many as 50% of these patients may incur some degree of cerebral vasospasm (2). Vasospasm typically occurs 7 to 10 days after hemorrhage, and its prompt diagnosis is required to initiate appropriate therapy to avoid is- chemic insults. The current standard of reference for detecting vasospasm is digital subtraction an- giography (DSA). DSA provides accurate depiction of the intracranial vessels and the aneurysm-clip

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