HT22 cells
Neuroprotective effect of Aronia melanocarpa extract against glutamate induced oxidative stress in HT22 cells
... (Rho123). HT22 cells were treated with 2 mM glutamate, 10 and 100 μg/ml of ...O-galactoside. HT22 cells were then stained with Rho123 for 15 min at 37 °C and ...
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Original Article Activation of mitochondria apoptotic pathway is involved in the sevoflurane-induced hippocampal neuronal HT22 cells toxicity through miR-145/Binp3 axis
... HT22 cells were transiently transfected with the firefly luciferase reporter plasmids and the Renilla luciferase-expressing plasmid (pRLSV- 40; Promega, Madison, USA), the latter as a transfection control, ...
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The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation
... Complex II dysfunction has been related to neu- rodegenerative diseases such as Huntington’s disease, as well as hypoxia-dependent generation of ROS in lungs [30]. In mice and rats, MA injection in the stria- tum ...
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Original Article Sevoflurane induces endoplasmic reticulum stress mediatedapoptosis inmouse hippocampal neuronal HT22 cells via modulating miR-15b-5p/Rab1A signaling pathway
... glioblastoma- cells [28]. Therefore, we hypothesized that Sev induces the HT22 cell apoptosis via modulating miRNAs ...in HT22 cells after treatment with different concentration of Sev ...
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Original Article Protective effect of β-hydroxybutyrate on glutamate induced cell death in HT22 cells
... in HT22 cells, the under- lying mechanism was not ...the HT22 cell line to explore the neu- roprotective effects of BHB and the mecha- ...nisms. HT22 cells lack ionotropic glutamate ...
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Neuroprotective effects of an Nrf2 agonist on high glucose-induced damage in HT22 cells
... The brain is especially sensitive to oxidative damage due to its high oxygen consumption rate, its abundant lipid content, and the relative paucity of antioxidant enzymes. Numerous studies have confirmed that dia- betes ...
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Acanthus ebracteatus leaf extract provides neuronal cell protection against oxidative stress injury induced by glutamate
... damaged cells, which converts the colorless tetrazolium salt, 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetra- zolium chloride (INT), into a red formazan ...product. HT22 cells were seeded at a ...
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Overexpression of Selenoprotein H Reduces Ht22 Neuronal Cell Death after UVB Irradiation by Preventing Superoxide Formation
... in Ht22 cells during oxidative stress (Panee et ...of cells from ROS, electrophiles and xenobiotics [44, ...in Ht22 cells after UVB irradiation via these ...
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Experimental Analysis of Interacting HT22 Plasma Membrane Cholesterol and β Amyloid
... the cells did not apoptose. We used HT22 hippocampal cells that are treated with a simian virus making them tumor ...hippocampal cells replicate, the effects of β -A can be compromised with ...
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Neuroprotection and spatial memory enhancement of four herbal mixture extract in HT22 hippocampal cells and a mouse model of focal cerebral ischemia
... Results: In the HPLC analysis, PMC-12 was standardized to contain 3.09 % 2,3,5,4 ′ -tetrahydroxystilbene-2-O- β -D-glucoside, 0.35 % 3 ′ ,6-disinapoyl sucrose, and 0.79 % catalpol. In HT22 cells, ...
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Effects of dimethyl fumarate on neuroprotection and immunomodulation
... hippocampal HT22 cells from oxidative glutamate toxicity (Figure 1A) in line with the increase in cellular glutathione levels seen in both the absence and presence of glutamate (Figure ...these cells ...
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Chemical constituents from a Gynostemma laxum and their antioxidant and neuroprotective activities
... on HT22 cells [33] and its hippocampus effects on hip- pocampus-dependent learning and memory in mice fed with different diets related with oxidative stress ...
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Nanotechnology in drug delivery: the need for more cell culture based studies in screening
... cued HT22 cells from oxidative stress-induced cell death [29], in another related study it was demonstrated to pro- tect normal human breast cells from radiation-induced apoptotic cell death ...
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Crocetin Prevents Amyloid β1 42 Induced Cell Death in Murine Hippocampal Cells
... tide to HT22 cells induced cell death as well as a pre- vious report [18]. Crocetin has been known to have po- tent anti-oxidant [19], anti-cancer [9], and anti-inflam- mation activities [8]. A number of ...
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<p>miR-195-Sirt3 Axis Regulates Angiotensin II-Induced Hippocampal Apoptosis and Learning Impairment in Mice</p>
... study, HT22 cells and wide-type mice were sub- jected to ANG II treatment to establish the hypertensive cell and mouse model, ...in HT22 cells and hippocampal tissues of hypertensive mice, ...
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Akt and mTOR mediate programmed necrosis in neurons
... in HT22 cells is dependent on ...in HT22 cells we used Mitosox red, which detects mitochondrial ROS as well as a number of other potential ROS ...Mitosox-positive cells were increased ...
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RIP1 and RIP3 mediate hemin-induced cell death in HT22 hippocampal neuronal cells
... the cells were digested, and part of them were seeded in 96-well plates at a density of 3,000 cells/well for hemin treatment whereas the other cells were kept in the incubator for 72 hours and then ...
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Sinomenine inhibits microglial activation by Aβ and confers neuroprotection
... microglial cells release sub- stances that can cause toxicity to ...hippocampal cells. For this purpose, we used a hippocampal cell line, HT22 that has been used in previous studies to examine ...
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Silver Nanoparticle Exposure Induced Mitochondrial Stress, Caspase-3 Activation and Cell Death: Amelioration by Sodium Selenite
... neuronal HT22 cells were used to delineate subcellular responses and mechanisms to AgNP by assessing the response levels of caspase-3, mitochondrial oxygen consumption, reactive oxygen species (ROS), and ...
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Regulation of cellular proliferation, differentiation and cell death by activated Raf
... expressing HT22 cells with BDNF triggered a robust, but transient activation of ERK that was not sufficient to con- fer protection against the loss of trophic ...neuronal HT22 cells from serum ...
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