human genomic dna
Effect of probe characteristics on the subtractive hybridization efficiency of human genomic DNA
... Findings: In this study we investigate how various capture probe characteristics influence the subtraction efficiency. While the primary focus of this report is the impact of probe length, we also studied the impact of ...
5
Extension and Modification of Kanai’s DNA Isolation Method for A Spectrum of Human Specimens Collected by Invasive and Noninvasive Methods Suitable for Genotyping Studies
... for human genomic DNA ...for DNA isolation from various human specimens (blood, clot, saliva, urine, and cell lines) and from Gram-negative bacterial ...The DNA isolated by this ...
8
Gene detection with synthetic oligonucleotide sequences
... the human genomic DNA, having first digested it and added linker/primers for PCR amplification and hybridizing with cDNAs in solution (after preblocking repeat sequences) and then capturing the ...
219
Gaitzsch, Erik (2019): Proinflammatorische und prothrombotische Effekte doppelsträngiger DNA am vaskulären Endothel. Dissertation, LMU München: Medizinische Fakultät
... of human endothelial cells with both poly (dA:dT), a synthetic analog of viral DNA, and human genomic DNA led to a significant induction of proinflammatory cytokines, chemokines and ...
57
Investigation of G72 (DAOA) expression in the human brain
... on human genomic DNA (Clon- tech; ...reaction), human adult total brain, amygdala, testis and fetal brain cDNA (Marathon Ready cDNA, Clontech; ...
14
Site specific cleavage of genomic DNA mediated by triple helix formation
... This thesis describes the application of oligonucleotide directed triple helix formation to bind unique target sites in bacteriophage A, yeast, and human genomic DNA.. Cleavage at[r] ...
294
The Evolution of Mammalian Olfactory Receptor Genes
... To test whether this gene count was artificially low due to the use of a probe from a different species, four replicate Southern blots of dog and human genomic DNA were hy[r] ...
11
Genes encoding pancreatic polypeptide and neuropeptide Y are on human chromosomes 17 and 7
... to human mRNAs encoding pancreatic polypeptide and neuropeptide Y were used to detect specific human genomic DNA sequences in human-mouse somatic cell hybrid ...with human ...
5
Expression of pro opiomelanocortin gene and quantification of adrenocorticotropic hormone like immunoreactivity in human normal peripheral mononuclear cells and lymphoid and myeloid malignancies
... a human genomic DNA probe for the pro-opiomelanocortin (POMC) gene, we have shown specific mRNA in normal human peripheral mononuclear cells (PBMC); the presence of specific mRNA was also ...
6
Monitoring human papillomavirus prevalence in urine samples: a review
... Whenever possible we extracted information on country, recruitment setting, age (range and mean), urine sampling procedure, total sample size, percentage of samples contain- ing human genomic DNA, ...
13
Circulating DNA in systemic lupus erythematosus Isolation and characterization
... a DNA isolation procedure that used a combination of proteolysis, phenol extraction, and hydroxylapatite adsorption and elution in the presence of ...plasma-derived DNA and human genomic ...
11
Gene deletions correlate with the development of alloantibodies in von Willebrand disease
... Normal human genomic DNA H, DNA from a mouse-human hybrid cell line that contains human chromosome 12 but not human chromosomes 9-11 or 22 M, or DNA from purified human chromosome 22 22 [r] ...
8
Locus Reference Genomic sequences: an improved basis for describing human DNA variants
... most human genomic DNA sequences represented in RefSeq have been of genomes rather than individual ...in genomic DNA coordinates using RefSeq genomic contig sequences has been ...
7
Dynamic instability of genomic methylation patterns in pluripotent stem cells
... retroviral DNA sequences with host restriction factors, which include TRIM28 [21] and ZFP809 ...of DNA methylation is impor- tant for stable proviral silencing [23], and 5-azacytidine- induced demethylation ...
10
Long Term Stability of Human Genomic and Human Papillomavirus DNA Stored in BD SurePath and Hologic PreservCyt Liquid Based Cytology Media
... The human beta-globin (HBB) gene was detected on one channel as an internal sample processing ...a human GAPDH primer set (G3PDH For/Rev primers; Integrated DNA Technologies ...
5
Specific Detection of Fusarium Species in Blood and Tissues by a PCR Technique
... peated sequences such as those of rDNA and mitochondrial DNA. For diagnostic purposes, it is essential that repeated sequences be used as targets to ensure good sensitivity, which justifies our choice of primers. ...
5
High performance of targeted next generation sequencing on variance detection in clinical tumor specimens in comparison with current conventional methods
... of DNA, and has high sensitivity and ...of DNA alter- ations from a large number of clinical specimens, which have been confirmed by the current clinical ...
12
Comparative mapping of Arabidopsis thaliana and Brassica oleracea chromosomes reveals islands of conserved organization.
... The map shows the linkage arrangement of 49 loci detected by 44 previously mapped Brassica PstI genomic DNA clones (SLOCUM et al.. Also, four Brassica PstI genomic DNA.[r] ...
12
Identification of CAR/RXRα Hetero dimer Binding Sites in the Human Genome by a Modified Yeast One Hybrid Assay
... the human genome, we performed a modified yeast one-hybrid assay that enables rapid and efficient identification of genomic targets for DNA-binding ...proteins. DNA fragments were recovered ...
15
Human Papillomavirus E6 Triggers Upregulation of the Antiviral and Cancer Genomic DNA Deaminase APOBEC3B
... A3B has been strongly implicated in mutagenesis in a wide variety of human cancers (29–37). Given its relatively low expres- sion level in almost all normal tissues (29, 30, 38), a major ques- tion is how A3B ...
8