Human iPSCs
Transcriptional Reactivation of OTX2, RX1 and SIX3 during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs
... [8]. iPSCs can differentiate into RPE cells that are highly similar to native RPE and induced RPE derived from ESCs ...of iPSCs in regenerative medicine en- counters several safety ...generated human ...
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Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages
... as human embryonic stem cells (hESCs), offer a great potential for application in medicine as they can be directed to differentiate into virtually any cell type of an ...of human iPSCs (hiPSCs) is ...
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Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model
... Methods: Human iPSCs were generated from normal dermal fibroblasts by transfecting plasmids encoding for six factors: OCT4, SOX2, KLF4, L-MYC, LIN28, and p53 ...the iPSCs were treated with GSK3i to ...
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Enhancement of recovery of Neisseria meningitidis by gelatin in blood culture media
... Undifferentiated human iPSCs were maintained in mTeSR medium (Stemcell Technologies) on Matrigel (Corning)-coated feeder-free ...differentiation, iPSCs were dissociated by Accutase (Stemcell ...
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Applications of Patient-Specific Induced Pluripotent Stem Cells; Focused on Disease Modeling, Drug Screening and Therapeutic Potentials for Liver Disease
... of human iPSCs Directed differentiation of human iPSC lines into hepatic cells has been improved with more efficient and functional methods including our multistage differentiation protocol (19, 21, ...
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Effects of cellular origin on differentiation of human induced pluripotent stem cell–derived endothelial cells
... passage human iPSCs, per- haps influencing the lineage differentiation potential and the application of iPSC derivatives in disease mod- eling and drug discovery (17, 36, ...in iPSCs (38), they did ...
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Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells
... of human PGCs from iPSCs or ...from human iPSCs and ESCs in the 4i culture system ...that human PGCs had underwent demethylation during the develop- ment period and the methylation ...
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Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey
... if iPSCs were disseminated in disease lesions, they would be controlled by tissue microenvironment and behave based on the needs of local ...including human, monkey, and mouse, we demonstrated that dis- ...
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OCT4 activity during conversion of human intermediately reprogrammed stem cells to iPSCs through mesenchymal epithelial transition
... interest. Human iRSCs might represent a suitable cell source for directed conversion without full reprogramming, and for dissecting the molecular mechanisms involved in these alternative ...of human ...
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Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes
... and human iPSCs ...trimester human cardiomyocytes, hiPSC-CM, and human embryonic stem cell-derived cardiomyocytes (hESC-CM) ...(BPEL). Human PSC-CM cultured in maturation media showed ...
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Toward a 3D model of human brain development for studying gene/environment interactions
... erentiating human iPSCs into neural progenitor cells (NPCs) and neuronal and astroglia lineages (Figure ...[25,33]. iPSCs are detached after treatment with collagenase (1 mg/ml) for 1 hour and plated ...
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Endothelial progenitor cells and neural progenitor cells synergistically protect cerebral endothelial cells from Hypoxia/reoxygenation-induced injury via activating the PI3K/Akt pathway
... that iPSCs have unlimited self-renewal ability and are able to differentiate to various types of cells with less ethical issues for clinical applications [23, ...differentiated human iPSCs into EPCs ...
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The use of induced pluripotent stem cells to reveal pathogenic gene mutations and explore treatments for retinitis pigmentosa
... in human iPSCs ...targeted iPSCs, and large gene targeting in iPSCs will be useful for establishing the pathogenesis of various candidate genes associated with hereditary diseases ...
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Generation of disease specific induced pluripotent stem cells from patients with rheumatoid arthritis and osteoarthritis
... predisposed human cells may allow scientists to study the pathophysiology of diseases with greater ...patient-derived iPSCs is now considered more representative of a disease than other ...generated ...
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Rapamycin regulates autophagy and cell adhesion in induced pluripotent stem cells
... from human iPSCs, with altered expression of adherens junctions and actin- modifying ...The iPSCs are supersensitive to enzyme dissociation and therefore the “hanging drop” technique is difficult to ...
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Mesodermal iPSC–derived progenitor cells functionally regenerate cardiac and skeletal muscle
... both iPSCs and MiPs (Figure ...in iPSCs and remodeled in MiPs (Supplemental Figure ...in iPSCs and MiPs, with no bias discriminating against f- and MAB-derived prog- enies (Q1 = NS; Figure 4E and ...
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Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis
... trypsin-EDTA (TE) at 70% confluence, and 10 μM rho-associated kinase (ROCK) inhibitor was added to the newly passaged cells. To differentiate into endoderm, STEMdiff definitive endoderm basal medium (STEM- CELL ...
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Optimizing the method for generation of integration-free induced pluripotent stem cells from human peripheral blood
... reprogramming iPSCs and have the potential to expedite advances in iPSC-based therapies ...integration-free iPSCs from human PB MNCs, we optimized the method of generating them from human PB ...
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Neurodegeneration in SCA14 is associated with increased PKCγ kinase activity, mislocalization and aggregation
... in human patient-derived induced pluripotent stem cells (iPSCs) carrying two distinct SCA14 mutations in the C1 domain of PKC γ , H36R and ...patient iPSCs and ...patient iPSCs were found to ...
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Establishment of porcine and human expanded potential stem cells
... porcine iPSCs (Extended Data Fig. 1f and 1h). Therefore mouse or human naï ve ESC conditions 5-7 cannot be directly extrapolated to porcine ...Dox-independent iPSCs (pEPSC iPS ) remained ...
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