Human papillomavirus 16
E6^E7, a Novel Splice Isoform Protein of Human Papillomavirus 16, Stabilizes Viral E6 and E7 Oncoproteins via HSP90 and GRP78
... ABSTRACT Transcripts of human papillomavirus 16 (HPV16) E6 and E7 oncogenes undergo alternative RNA splicing to produce multiple splice isoforms. However, the importance of these splice isoforms is ...
15
Vesicular Trafficking of Incoming Human Papillomavirus 16 to the Golgi Apparatus and Endoplasmic Reticulum Requires γ Secretase Activity
... E6/E7-immortalized human 2614 ectocervical keratinocytes and HaCaT cells, a spontaneously im- FIG 2 The FLAG tag at the C terminus of HPV16 L2 is constitutively ...
11
Vimentin Modulates Infectious Internalization of Human Papillomavirus 16 Pseudovirions
... FIG 2 Identification of cell surface vimentin as a binding protein for HPV16-PsVs. (A) Schematic outline of the pulldown experiment to isolate FPC HPV16-PsVs-binding surface proteins. (B) Silver-stained SDS-PAGE gel of ...
17
Characterization of the Human Papillomavirus 16 E8 Promoter
... [Fig. 3A]) were greatly impaired in their response to E1/E2 (Fig. 5). The E1/E2-induced activity of the 7154/1268 construct was also highly dependent on URR sequences, as the 863/1268 con- struct did not respond to E1 ...
10
Integration of human papillomavirus 16 in esophageal carcinoma samples
... life cycle. In many HPV-infected patients, HPV E2 restrains E6 and E7 gene transcription, which aids in the regulation of cellular proliferation [27]. Cleaving the E2 gene increased HPV E6 and E7 gene expression, which ...
9
Human Papillomavirus 16 (HPV-16), HPV-18, and HPV-31 E6 Override the Normal Phosphoregulation of E6AP Enzymatic Activity
... Previous studies had shown that the E6AP T485 residue is a phosphoacceptor site for PKA, with the nonphosphorylatable mutant T485A demonstrating increased levels of ubiquitination activity, both with respect to an E6AP ...
12
Combined Transcriptome and Proteome Analysis of Immortalized Human Keratinocytes Expressing Human Papillomavirus 16 (HPV16) Oncogenes Reveals Novel Key Factors and Networks in HPV-Induced Carcinogenesis
... immortalized human keratinocytes (normal oral keratinocytes [NOKs]) (29) with a well-defined expression of HPV16 oncogenes and the corresponding parental negative-control ...
23
Modulation of microRNA mRNA Target Pairs by Human Papillomavirus 16 Oncoproteins
... Processing and analysis of RNA-seq reads. Downstream analysis of the sequenced reads from each sample was performed with a unique in-house pipeline designed by GSL. Briefly, quality control checks of raw sequence data ...
14
Maturation of the Human Papillomavirus 16 Capsid
... keratinocytes. Papillomavirus virions are released into the en- vironment through a process known as desquamation, in which keratinocytes lose structural integrity prior to being shed from the surface of the ...
11
α Defensin HD5 Inhibits Human Papillomavirus 16 Infection via Capsid Stabilization and Redirection to the Lysosome
... -defensin human defensin 5 (HD5) inhibited host furin cleavage of the minor capsid protein L2 of HPV16 at the cell surface and postulated that blocking this cleavage disrupted L2 functions required for produc- ...
14
Membrane Orientation of the Human Papillomavirus Type 16 E5 Oncoprotein
... of human papillomavirus type 16 is a small, hydrophobic protein that localizes predomi- nantly to membranes of the endoplasmic reticulum ...and human ectocervical cells, the C terminus of E5 ...
8
Regression of Established Human Papillomavirus Type 16 (HPV-16) Immortalized Tumors In Vivo by Vaccinia Viruses Expressing Different Forms of HPV-16 E7 Correlates with Enhanced CD8+ T-Cell Responses That Home to the Tumor Site
... molytic portion of LLO (24). We recently described (G. Gunn et al., submitted for publication) a potent E7-based immuno- therapeutic agent that could cause the regression of estab- lished HPV-16 immortalized ...
11
Human Papillomavirus Type 16 E5 Protein as a Therapeutic Target
... platelet-derived growth factor β (PDGF ) β 14 and colony stimulating factor (CSF). The E5 protein has transforming activity in HPV-16 infected cells. In the protein-encoding regions, E6 and E7 ORFs are considered ...
14
Functional Mapping of the Human Papillomavirus Type 16 E1 Cistron
... We then incorporated the E2 site mutations listed above into plasmid constructs that could delineate the effect of these cis sequence alterations on E1 expression or ori function in complementation assays (Fig. 6B). ...
11
Mutational analysis of human papillomavirus type 16 E7 functions.
... Whereas the immortalizing function transformation in conjunction with activated ras for primary rat cells paralleled the transforming function for rat 3Y1 cells, the transformation funct[r] ...
8
Perturbation of the p53 response by human papillomavirus type 16 E7.
... inhibition of most cyclin-dependent kinase function in E7- expressing cells indicates that the inhibitor is functional. Al- though the function of cyclin E- and cyclin A-associated ki- nases is normally essential for ...
9
Antiviral activity of Bifidobacterium adolescentisSPM1005-A on human papillomavirus type 16
... Among commensal bacteria, Bifidobacteria is one of the most numerous probiotics in the mammalian gut that belong to LAB [14]. Xiao et al. have reported choles- terol reduction by a supplement containing Bifidobacter- ium ...
6
Human Papillomavirus Type 16 E7 Oncoprotein Associates with E2F6
... Cervical cancer is the second most common cancer among women worldwide and is the most common female cancer in developing countries (46, 55). The most significant risk factor for cervical cancer is infection with ...
11
“Development of Prophylactic Bivalent and Quadrivalent Human Papillomavirus (HPV) Vaccines: A Review” by Vikrant C. Sangar, Balasaheb B. Ghongane, India.
... bovine papillomavirus (BPV) types 1 and 2 by the induction of transformed foci in NIH3T3 and C127 mouse cell ...infectious human papillomavirus type ...
7
Mechanisms of Human Papillomavirus-Induced Oncogenesis
... of human solid tumors (reviewed in reference ...in human tumors have more recently been complemented by studies designed to define the mini- mally necessary series of oncogenic steps necessary to generate ...
10