Human Pluripotent Stem Cell
Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes
... As previously discussed, while hESC-CMs are metabolically active, they possess an immature metabolic profile that is predominantly glycolytic. In normal cardiac development, metabolic or mitochondrial maturation does not ...
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Modulating the biochemical and biophysical culture environment to enhance osteogenic differentiation and maturation of human pluripotent stem cell-derived mesenchymal progenitors
... in cell cycle, DNA replication, spin- dle assembly, mitotic division, and carcinogenesis, suggesting a strong repression of proliferation associated with culture in ...
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The advancement of human pluripotent stem cell derived therapies into the clinic
... Scaling cell production, controlling differentiation, achieving successful engraftment and demonstrating efficacy are challenging ...clinical-grade stem cell derivatives which show good efficacy in ...
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CRISPR Genome Engineering for Human Pluripotent Stem Cell Research
... model cell development in a ...defective stem cell ...of human cell-based experiments. This is because human stem-cell based models, recapitulating specific ...
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A Pluripotent Stem Cell Model of Human Placental Development.
... hESC cell line (H9) that expresses an inducible shRNA against YAP ...This cell line along with a scrambled shRNA control were the kind gift from ...high cell death was observed (Figures ...
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Selection of human induced pluripotent stem cells lines optimization of cardiomyocytes differentiation in an integrated suspension microcarrier bioreactor
... offline cell washes and product ...improve cell viability, as well as minimize medium utilization, optimization of the feed rate was ac- complished by employing metabolic activity-based feed- ing, based on ...
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Understanding Megakaryopoiesis And Thrombopoiesis Using Human Stem Cells Models
... Human stem cell models (CD34+ hematopoietic progenitors, embryonic stem cells and induced pluripotent stem cells (iPSCs)) are powerful tools for the study of megakaryopoiesis and ...
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Effects of mechanical stimulation on the reprogramming of somatic cells into human-induced pluripotent stem cells
... Human dermal fibroblast cells were seeded on commercial plates or BioFlex (Flexcell Corp., Hillsborough, NC, USA) at a density of 8 × 10 3 cells/cm 2 . Then, 1 day after seeding, the concentrated supernatant was ...
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Establishment of porcine and human expanded potential stem cells
... potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation 1 ...germ cell- like cells in vitro. Under similar conditions, ...
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Pluripotent stem cells for Parkinson's disease: progress and challenges
... by human trials using fetus- derived DA neurons, provide compelling evidence to justify the development of these cells for use in human clinical ...between human and rat cells in the brain or a ...
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3D brain Organoids derived from pluripotent stem cells: promising experimental models for brain development and neurodegenerative disorders
... induced cell death in neuroepithelial progenitors, attenuated the proliferation of progenitor cells, and increased the size of lumen within ventricular ...ZIKV-infected human fetal brain ...
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Human development, heredity and evolution
... in human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technology have enabled the generation of human organoids (miniature versions of human ...
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Exosomes Secreted from Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Osteonecrosis of the Femoral Head by Promoting Angiogenesis
... Stem cell transplantation has been proven to be able to promote angiogenesis in ONFH [7, ...by stem cells have been demonstrated to show similar therapeutic effects on ischemic ...mesenchymal ...
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Pluripotent Human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy
... the cell via- bility of SH-SY5Y cells was quite resistant to EV71 infec- tion ...the cell culture supernatant with qRT-PCR, even though this was still the lowest amongst the four different cell types ...
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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... Real-Time Cell Analysis (RTCA) Cardio system, which utilizes impedance tech- nology to quantify CM-beating properties, has been previously reported as an emerging method to quantify cardiac contractility ...
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The potential and limitations of induced pluripotent stem cells to achieve wound healing
... multiple cell types and ...therapies. Stem cell therapy is emerging as a potential treatment for chronic wounds, and adult-derived stem cells are currently employed in several commercially ...
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Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes
... adult stem cells, and human pluripotent ...of cell junction proteins such as Cx43 and N-Cadherin ...the cell poles compared to unstretched cells, which maintain an even dispersion ...
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Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D
... pluripotent stem cells (iPSC) are unlimited and stable sources with the potential to differentiate into any cell type in the body. MSC have been previously derived from hESC via co-culturing with ...
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Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells
... of stem cell differentiation (or direct cardiac reprogramming from fibroblasts [153]) towards cardiac lineage and screening of factors to enhance maturation and homogeneity of derived populations for ...
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Disruption of histamine/H1R signaling pathway represses cardiac differentiation and maturation of human induced pluripotent stem cells
... of cell suspension (2 × 10 5 cells) containing derived CMs with histamine or with- out histamine was injected into the border zone of the infarcted heart, and each mouse was injected with three ...after ...
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