human pluripotent stem cell differentiation
Mapping human pluripotent stem cell differentiation pathways using high throughput single cell RNA sequencing
... stromal cell clus- ter ...each cell type were enriched for the expected Gene Ontol- ogy (GO) terms (Additional file 1: Figure S2c) ...muscle cell cluster are significantly enriched for skeletal ...
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Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions
... pancreatic cell progeny may reveal clues about pathways engaged in the transition of hPSCs toward islet cells and their ...the differentiation of hPSCs to pancreatic ...pancreatic cell-specific ...
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Pluripotent Human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy
... neural differentiation markers: (PAX6, Musashi1, & βIII-tubulin ...neural differentiation markers NCAM and NSE ...derived cell lines displayed aberrant expression of various neural attachment ...
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Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
... both pluripotent hPSC expansion (in the commercially available culture media, StemPro and mTeSR1) and subsequent multilineage differentiation into representa- tives of the three germ layers, namely ...
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Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea
... hair cell-like cells and SGNs In cochlea, hair cells are connected with SGNs facilitat- ing the conduction of signals between ...hair cell-like cells and SGNs, induced hair cell-like cells were ...
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Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells
... Adult stem cells reside within specialized microenvironments in which they are kept undifferentiated due to secreted signals from surrounding cells, cell-cell interactions and adhesion to ...
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Transplantation of human ESC-derived mesenchymal stem cell spheroids ameliorates spontaneous osteoarthritis in rhesus macaques
... 26]. Pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSC) have an unlimited self-renewal capability and can differentiate into ...
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p53 switches off pluripotency on differentiation
... in human [2, 3] and ...of human iPSC generation ...Klf4, human fibroblast cells were de-differentiated into pluripotent stem cells but can dramatically be switched off by overexpressing ...
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Pluripotent stem cell differentiation as an emerging model to study human prostate development
... androgen hormones and then exerts its function as a tran- scription factor [19]. Immediately after sex determination, dihydrotestosterone converted from testosterone by 5α- reductase binds to AR to activate the ...
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Effects of cellular origin on differentiation of human induced pluripotent stem cell–derived endothelial cells
... EC differentiation efficiency, we assumed EC-iPSCs might retain EC marker gene expression in ...donor cell– derived iPSCs before and after differentiation, using fetal primary ECs as a positive ...
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Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells
... successful differentiation of human PGCs from iPSCs or ...in pluripotent cell states can be induced by environmental factors in respect to the gain and loss of competence for germ cell ...
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The ACVR1 R206H mutation found in fibrodysplasia ossificans progressiva increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling
... directed differentiation protocol to create human induced pluripotent stem cell (hiPSC)- derived endothelial cells (iECs) from patients with fibrodysplasia ossificans progressiva (FOP), ...
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Disruption of histamine/H1R signaling pathway represses cardiac differentiation and maturation of human induced pluripotent stem cells
... on human cardiomyo- genesis, we directed hiPSCs to undergo stepwise differ- entiation into cardiomyocytes according to a classical protocol ...myocardial differentiation, different concentrations of ...
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In Pursuit of Porcine Pluripotent Stem Cells for Autologous Cell Therapy
... vitro differentiation of presumptive porcine iPSC into rod photoreceptors and their integration into the retinas of recipient pigs has been reported ...of stem cell-based treatment to recover the ...
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Wnt/β catenin signaling directs the regional expansion of first and second heart field derived ventricular cardiomyocytes
... regenerative cell-based therapies. We have used murine and human pluripotent stem cell systems to demonstrate that, during in vitro cellular differentiation, early ventricular ...
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Transcriptional Reactivation of OTX2, RX1 and SIX3 during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs
... Directed differentiation of human induced pluripotent stem cells (iPSCs) into retinal pigmented epithelium (RPE) holds great promise in cell replacement therapy for patients suffering ...
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Human pluripotent stem cell-derived cardiomyocytes for heart regeneration, drug discovery and disease modeling: from the genetic, epigenetic, and tissue modeling perspectives
... Genetic manipulation and alteration in epigenetic regulation through chromatin remodeling also control cell fate. Viral transduction of Gata4, Mef2c, and Tbx5 (GMT) directly transdifferentiates murine fibroblasts ...
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Friedreich's ataxia induced pluripotent stem cell derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency
... control human embryonic stem cell (hESC) line H9 (WiCell) [36] were maintained in the undifferentiated state using TeSR-E8 medium (Stem Cell ...latter. Differentiation into ...
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Human endogenous retrovirus K (HML 2) RNA and protein expression is a marker for human embryonic and induced pluripotent stem cells
... undifferentiated human ESCs and iPSCs. Induction of differentiation by embryoid body formation resulted in rapid silencing of HERV-K(HML-2) provirus ...individual cell lines. These proviruses are ...
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Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D
... pluripotent stem cells (iPSC) are unlimited and stable sources with the potential to differentiate into any cell type in the ...trophoblast differentiation followed by multiple rounds of ...
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