Human somatic nuclear transfer (SCNT)
Use of gene-editing technology to introduce targeted modifications in pigs
... of human diseases, making them a useful model in ...in somatic cells and somatic cell nuclear transfer (SCNT) to generate genetically engineered (GE) pigs carrying site-specific ...
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Cellular reprogramming in farm animals: an overview of iPSC generation in the mammalian farm animal species
... (especially human) for ESC derivation faces ethical concerns and is subjected to rigorous legal ...Reprogramming somatic cells by SCNT is technically challenging, laborious, inefficient (especially ...
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Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
... bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early development stage based on ...
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Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology
... reported to date, and all the evidence supports the hy- pothesis that MC3R is important for human energy homeostasis [10–13]. Nevertheless, the pathogenic role of MC3R in human obesity remains controversial ...
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Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
... Although studies with Hela cells have helped establish the screening protocols for selecting CFTR-targeted clonal cell lines, approaches for targeting primary fetal ferret fibrob- lasts have proven more difficult due to ...
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Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio)
... in somatic cell reprogramming of human pluripotent stem cells [25, ...in somatic cell reprogramming, which improved somatic cell induction efficiency by blocking the degradation of miRNA to ...
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Generation of GHR modified pigs as Laron syndrome models via a dual sgRNAs/Cas9 system and somatic cell nuclear transfer
... the human syndrome, including severe postnatal growth retarda- tion, proportionate dwarfism, absence of the GHR bind- ing protein, greatly decreased serum IGF-I and elevated serum GH concentrations ...between ...
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Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer
... sporadic human cancers [7]. It was estimated that approximately 80% of human tumors have dysfunctional ...of human cancers, indicating that this tumor suppressor exerts a protective role against ...
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SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo
... Pig Oocyte Collection and In Vitro Maturation (IVM) Porcine ovaries obtained from a slaughterhouse were de- livered to the laboratory in a thermos containing 0.9% saline and at 36 °C. Within 2 h, 3–6-mm ovarian follicles ...
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Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors
... in human-bovine embryos [8], the blastocyst stage in macaque-rabbit embryos [10] and in a small minority of caprine- ovine embryos (4/40; range = 2 to 20+cell embryos; ...the human and murine mtDNA genomes ...
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Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear transferred embryo development in the intraooplasmic karyoplast injection technique: a review
... the somatic cloning technique. The alternative strategy of nuclear-cytoplasmic hybrid creation, which has been proposed by Lee et ...whole somatic cell bypassing both the do- nor cell-cytoplast ...
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Specific genetic modifications of domestic animals by gene targeting and animal cloning
... [43]. Human and old world monkeys have natural antibodies against α (1, 3)-galacto- syl epitopes on pig ...when human serum bound to cells and tissues of these mice they showed sub- stantially less ...
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Generation of bovine transgenics using somatic cell nuclear transfer
... a human disease in which understanding may be advanced through the creation of a porcine or bovine model, and is currently underway in our labora- tory, is ataxi telangiectasia ...
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Coexistence of Bos taurus and B. indicus Mitochondrial DNAs in Nuclear Transfer-Derived Somatic Cattle Clones
... Moraes, C. T., L. Kenyon and H. Hao, 1999 Mechanisms of human Thompson, J. G., C. McNaughton, B. Gasparrini, L. T. McGowan mitochondrial DNA maintenance: the determining role of pri- and H. R. Tervit, 2000 Effect ...
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The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos
... The mammalian zygote is able to develop up to the 4-cell or 8-cell embryonic stage (species specific) without major genome activation. The proper cell division is maintained and controlled by maternally inherited factors ...
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Bähr, Andrea (2011): (Re)producing transgenic pigs for xenotransplantation – selection of founder animals and establishment of breeding herds. Dissertation, LMU München: Tierärztliche Fakultät
... diseased human lung, or the effect of therapeutics, in many ways (Rogers et ...and human lungs (Jones et ...the human cystic fibrosis lung, especially during the neonatal period before onset of ...
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Choice of random rather than imprinted X inactivation in female embryonic stem cell derived extra embryonic cells
... Robertson, 1989). However, we previously showed that the forced expression of the homeobox transcription factor Cdx2 triggers the differentiation of ES cells toward TE (Niwa et al., 2005). It is also possible to induce ...
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Gene expression of OCT4, SOX2, KLF4 and MYC (OSKM) induced pluripotent stem cells: identification for potential mechanisms
... To drive somatic cells to the pluripotent state, viral transduction of OSKM factors is considered as the most robust method. Despite this, we do not fully elucidate the molecular mechanisms of reprogramming which ...
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The influence of donor nucleus source on the outcome of zebrafish somatic cell nuclear transfer
... embryos. For the HGn30A, HG21C, and HGn8E lines, the embryos were dissected and selected for the GFP+ tissues. For the HGn28A and HGn62A lines, the whole embryos were extracted from the yolk prior to use. Subsequently, ...
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The Influence of Genetic Background on Stem Cell Derivation and Variability.
... The natural variation in coat color that was appreciated and used by early researchers was only one aspect of mouse variation. Early breeding experiments lead to the production of inbred mouse strains, many of which ...
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