Human THP-1 monocytes generation and maintenance
I. Ion Channels in Human THP-1 Monocytes
... idly as the solutions were changed (on the order of 10 sec). For Cs + and Ba 2+ , block was much greater at more negative potentials. The outward current was reduced in an apparently voltage-independent manner, but this ...
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II. Voltage-activated Proton Currents in Human THP-1 Monocytes
... Received: 19 September 1995/Revised: 14 March 1996 Abstract. Depolarization-activated H + -selective currents were studied using whole-cell and excised-patch voltage clamp methods in human monocytic leukemia ...
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Human Cytomegalovirus Chemokine Receptor Gene US28 Is Transcribed in Latently Infected THP-1 Monocytes
... DISCUSSION To date, both US28 transcripts and CLTs are the only tran- scripts identified in myeloid cells that are latently infected with HCMV. Yet, they are also present in productively infected cells (4, 24, 42, 43, ...
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Quantification of activity of inflammatory and apoptotic caspases in THP-1 cells and monocytes
... differentiated monocytes from peripheral blood of healthy donors and on THP-1 cells, and to determine if they cause activation of inflammasome and programmed cell death ...on THP-1 ...
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Intracellular Infections Enhance Interleukin-6 and Plasminogen Activator Inhibitor 1 Production by Cocultivated Human Adipocytes and THP-1 Monocytes
... of THP-1 monocytes added to the ...by THP-1 cells or cocultures, whereas incubation of adipocytes and cocultures with conditioned medium from CMV-infected THP-1 cells led ...
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Evidence for hydroxyl radical generation by human Monocytes
... ethylene generation from methional, cell preparations of human monocytes were demonstrated to generate hydroxyl radical or a similar agent during phagocytosis of zymosan ...The generation of ...
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Biological effects of double-walled carbon nanotubes on the innate immune system: An in vitro study on THP-1 human monocytes
... of THP-1 monocytes with increased CD11b and CD14 ...nanotubes 1 m m or more in length, while endocytosis or diffusion were attributed to smallest objects ( Raffa et ...by THP-1 ...
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Inhibition of NF-kB activation and cytokines production in THP-1 monocytes by 2-styrylchromones
... modulation triggers a cascade of signaling events, namely the expression of many cytokines, enzymes, chemokines, and adhesion molecules, some of which being potential key targets for intervention in the treatment of ...
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Amyloid-β peptide-induced extracellular S100A9 depletion is associated with decrease of antimicrobial peptide activity in human THP-1 monocytes
... and monocytes are the most abundant cell types, and are re- leased following neutrophil necrosis ...and human monocytes secreted significant amounts of S100A8 [33,34], this study has shown that ...
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Human immunodeficiency virus type 1 envelope protein does not stimulate either prostaglandin formation or the expression of prostaglandin H synthase in THP-1 human monocytes/macrophages.
... or human anti-PGHS-2 polyclonal ...CD4 1 and are major sites of HIV infection. The THP-1 human monocyte cell line was chosen since these cells are CD4 1 and may serve as a model ...
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Flavonoid metabolites reduce tumor necrosis factor α secretion to a greater extent than their precursor compounds in human THP 1 monocytes
... were stimulated with 1 g/mL LPS for 24 h for IL-1 and IL-10 production assays. Cytokine concentrations in super- natants were quantified using human ELISA kits from BD Biosciences (Oxford, UK). ...
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Phellinus linteus polysaccharide extracts increase the mitochondrial membrane potential and cause apoptotic death of THP-1 monocytes
... treated THP-1 cells, ...in THP-1 ...of THP-1 cells upon treatment with ...by human serum, it is likely that the apoptosis of THP-1 cells induced by the ...
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Effect of PMA on Autophagy in Human Monocyte Cell-Line, THP-1
... INTRODUCTION:- THP-1 cells are human monocyte leukemia cells that were obtained from a one year old leukaemia patient (Tsuchiya et ...of monocytes and macrophages, upon discovery 35 years ...
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III. Ion Channel Expression in PMA-differentiated Human THP-1 Macrophages
... As monocytes differentiate into mature, nondividing macrophages they become capable of a large variety of responses, including chemotaxis, phagocytosis, and se- cretion of numerous cytokines and other ...the ...
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Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
... of monocytes differentiated into mature DCs upon reverse transmigration across an endothelial barrier within 48 hours ...GM-CSF-cultured monocytes activated with calcium ionophore or CD40L rapidly express ...
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Eradication of intracellular Francisella tularensis in THP-1 human macrophages with a novel autophagy inducing agent
... ication by inhibiting the activation of autophagy with bac- terial secretory proteins [32-34]. The findings presented here indicate that intracellular F. tularensis at 12 h post- infection is not susceptible to the ...
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Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry
... Figure 1 shows the effect of EXEG 1706 treatment on THP-1 cell membrane permeability by PI exclusion ...cells-undifferentiated THP-1 (monocytes) with and without the addition of ...
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Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils
... Monocytes bound unaggregated IgG1 and Ig3 better than Ig4 whereas the binding of proteins of other classes was insignificant. Neutrophils bound unaggregated IgG1 and IgG3 proteins and, in addition, IgA1, IgA2, ...
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A proteomic analysis of C-reactive protein stimulated THP-1 monocytes
... 50% methanol for 30 min followed by incubation in 100 μl of 75% acetonitrile (ACN, Lab-Scan Analytical Sciences, Ireland) for a further 30 min. After drying the gel plugs (40°C, 23 min), 10 μ l of sequencing grade tryp- ...
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Release of human immunodeficiency virus by THP-1 cells and human macrophages is regulated by cellular adherence and activation.
... Ten days after infection with HIV-1RF, THP-1 cells were washed three times in PBS to remove all cell-free virus, resuspended in fresh medium, and cultured in nonadherent conditions in ti[r] ...
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